Nobody downloaded yet

Molecular Fluorescence Analysis of Proteins - Lab Report Example

Comments (0) Cite this document
Summary
Molecular absorption spectroscopy is a chemical technique that enables the qualitative and quantitative analysis of atoms or molecules by utilizing the absorption, emission and dispersion of electromagnetic radiations (Valeur & Berberan-Santos 10). The associations between…
Download full paperFile format: .doc, available for editing
GRAB THE BEST PAPER95.4% of users find it useful
Molecular Fluorescence Analysis of Proteins
Read TextPreview

Extract of sample
"Molecular Fluorescence Analysis of Proteins"

Download file to see previous pages As the electron returns from the lowest triplet state to the singlet ground state, a photon is released. The high sensitivity and specificity of fluorescence techniques facilitates the study of individual protein molecules.
Therefore, this experiment sought to determine the source of fluorescence in serum albumin by first establishing the fluorescence of two key amino acids that make up serum albumin. Such a study would enable a detailed study of serum albumin as a protein. The experiment also aimed at establishing the concentration of riboflavin using the absorption of known concentrations of riboflavin.
Solutions of 10 µM tyrosine and tryptophan were made to volumes of 100µL each by first preparing 100 mL of 1mM solutions and diluting them to 10 µM. The applications software of the fluorescent instrument was loaded after which the condition parameters were set. An emission scan range of between 290 and 380 nm was set alongside slits of 5nm. The emission wavelength was set to 280 nm, and the emission tab was clicked to obtain the emission spectrum of the buffer solution. The spectrum was saved after which the same procedure was repeated for tryptophan. Thereafter, an excitation spectrum for tryptophan was prepared by choosing the optimal emission wavelength according to the results of the emission spectrum. A tyrosine spectrum was obtained using similar conditions as those of the buffer. The tyrosine band was then obtained by subtracting the buffer spectrum from the tyrosine spectrum. All these steps were repeated for serum albumin making sure to save all the spectra.
1.0 mL of 10 ppm riboflavin solution was measured into a 100 mL volumetric flask and diluted using the buffer to make a 100 ppb stock solution. Standard solutions were then prepared using clean volumetric pipettes rinsed with HPLC water. The final concentrations of the standard solutions were ...Download file to see next pagesRead More
Cite this document
  • APA
  • MLA
  • CHICAGO
(“Molecular Fluorescence Analysis of Proteins Lab Report”, n.d.)
Molecular Fluorescence Analysis of Proteins Lab Report. Retrieved from https://studentshare.org/chemistry/1663975-molecular-fluorescence-analysis-of-proteins
(Molecular Fluorescence Analysis of Proteins Lab Report)
Molecular Fluorescence Analysis of Proteins Lab Report. https://studentshare.org/chemistry/1663975-molecular-fluorescence-analysis-of-proteins.
“Molecular Fluorescence Analysis of Proteins Lab Report”, n.d. https://studentshare.org/chemistry/1663975-molecular-fluorescence-analysis-of-proteins.
  • Cited: 0 times
Comments (0)
Click to create a comment or rate a document
CHECK THESE SAMPLES - THEY ALSO FIT YOUR TOPIC
Proteins
...?Proteins, lab report Proteins are essential nutrients and are therefore significant to the body, a factor that identifies the need to understand their presence in substances for nutritional, and other, purposes. An understanding of protein levels in the body is also important in determining changes from equilibrium fluid compositions. There are many ways in which protein concentrations in substances, particularly solutions, can be determined. The most common approach is the application of a regression analysis model to develop a relationship between protein concentration in a solution and absorbance rate that can be experimentally...
5 Pages(1250 words)Lab Report
X-ray Fluorescence
...X-Ray Fluorescence Introduction X-rays were discovered by Wilhelm K. Rontgen (1845 – 1923), a German physicist (Shackley 7). These rays have a long history in commercial elemental analysis since the discovery of the correlation between a sample’s atomic weight and the X-rays radiating from it, a fact that enabled Henry G. J. Moseley number elements in 1913 through the observation of K-line transitions as observed in X-ray spectrum. This formed the basis of element identification through X-ray fluorescence spectroscopy by considering the relationship between the atomic number and the frequency. X-Ray fluorescence, XRF refers to the emission of characteristic secondary,...
6 Pages(1500 words)Essay
Fluorescence and Pharmaceuticals
...How can the Phenomenon of Fluorescence be Used to Study the Effect of Pharmaceuticals on Cell Systems Introduction: Fluorescence illumination and observation is one of the most rapidly expanding technologies of our time when it is concerned with the staggering achievements of science and technology in the fields of Medicine, Biochemistry, Biotechnology, and Pharmaceuticals (Young M.R., 1961). The observation of fluorescence and analysis of its implications as applied to the domain of Biology has lead to development of more sophisticated technologies and numerous technological accessories dependent on fluorescence (Tobie, J.E., 1958). Physicochemical Principles: Fluorescence can be called a family of processes... , meaning...
7 Pages(1750 words)Essay
Molecular Identity
..., tagged with fluorescent spores like FITC-conjugated goat anti-rabbit IgG and the fluorescence induced by the reactions are focused on by special high-calibre microscopes and quantified by special software like TINA (Rivera, 2004). The proteins may be expressed first with suitable mutated primer sections using methods of identification and purification such as reverse transcriptase polymer chain reaction (RT-PCR) analysis (Rivera, 2004). More details of the process is not essential as the research report will provide a more comprehensive account. Conclusion The principal aim of this paper is to introduce a research...
12 Pages(3000 words)Essay
Proteins
...Proteins: Scientists have identified several factors that help lengthen the span of natural human lives. These include lifestyle factors, nutrition intake and exercise. By striking the right balance, one can live more than a hundred years as thousands of living centurions show. The objective of the book being analyzed is to help the reader appreciate the positive effects of certain essential proteins called Enzymes. Once we understand the efficacy and effectiveness of these enzymes we will have a newfound alternative to maintaining our health and preventing chronic age-related conditions. Enzymes can be defined as catalysts that cause a chemical reaction to process at a quicker pace. For instance,...
2 Pages(500 words)Essay
Molecular
...2nd October Problems for Chapter 3 C.3.6 Dice Suppose that you roll three fair dice. What is the probability of getting a five on at least one of the dice? Total outcomes in a dice {1 2 3 4 5 6} Probability of getting a five on one dice {event X) = 3 No. of possible outcomes in one dice = 3{1 2 3 4 5 6} = 18 P (X) = = In 3 dices the probability of getting at least a 5 = 3/18 = 1/6 C. Forensic DNA testing DNA found at a crime scene can be compared with DNA from suspected criminal to estimate the likelihood that both samples came from the same person. An enzyme is used to cut each DNA into short fragments; the enzyme always cuts at the same DNA sequence, and the positions of such sequences differ among individuals... October...
5 Pages(1250 words)Speech or Presentation
Proteins
...Protein Assay by the Bradford Method Quantitative determination of proteins is essential in many fields of protein study Bradford, 1976). It is worth noting that there is no single most significant protein assay method known to cause utterly perfect results. This, thus, means that it all depends on the desired speed, sensitivity accuracy, the nature of protein, as well as the nature of other components in the protein sample. One such true and accurate method of protein concentration determination involves using the acid hydrolyzing of a portion of sample and carrying out amino acid analysis on the hydrolyzate (Bradford, 1976). However, this method is somewhat expensive and a time consuming. There are various methods of protein... & Bailey,...
5 Pages(1250 words)Lab Report
Molecular Biology and Molecular Diagnostics
..., therefore the language employed is standard and can be comprehended by any age group, individual. This paper aims at exploring the form and nature of molecular biology with particular interest in understanding the interactions between the distinct systems of a cell. The paper will look at the interaction between RNA, DNA, protein biosynthesis, and how the interaction can be used in the diagnosis of a named disease. Molecular biology is concerned with the development, structure, and function of macromolecules critical to life (Carson, Miller, and Witherow, 2012, p. 19). The field digs deeper to explore the role of macromolecules in cell replication and how genetic information is...
2 Pages(500 words)Essay
Fluorescence
...5. Time-resolved and temperature-dependent stationary measurements of fluorescence will help determine the equilibrium constant. 6. Increasing the solvent polarity lowers the solvent-relaxed energy level and increases solvent re-orientation with respect to the fluorophore dipole in the excited state. 7. Solvents with low viscosity are good in obtaining transient effect in diffusion processes since lowest primary recombination yields are observed. Long fluorescence lifetime will be good to use. 8. De-aeration using nitrogen works by reducing the partial pressure of oxygen whereby the equilibrium is shifted from dissolved oxygen towards oxygen in the gas phase. Works Cited Arnaut, Luis, Sebastiao...
2 Pages(500 words)Lab Report
Portable X-ray fluorescence
...of environmental samples including estuary soils and sediments, contaminated soils, and biological samples. For the third experiment the major objective was to compare lead concentrations in surface peat samples from the South Pennines (UK) derived using (a) FPXRF in the field, (b) FPXRF in the lab on dried samples and (c) ICP-OES analysis. Introduction Theory X-ray fluorescence spectrometry is a core aspect in analysis especially in the field of science and industry application. The concept of XRF lies on the principle that individual atoms, wherever excited as a result of contact with an external energy source, ends up emitting X-ray photons characterized by a a wavelength or...
10 Pages(2500 words)Research Paper
sponsored ads
We use cookies to create the best experience for you. Keep on browsing if you are OK with that, or find out how to manage cookies.
Let us find you another Lab Report on topic Molecular Fluorescence Analysis of Proteins for FREE!
logo footer
Contact us:
+16312120006
Contact Us Now
FREE Mobile Apps:
  • StudentShare App Store
  • StudentShare Google play
  • About StudentShare
  • Testimonials
  • FAQ
  • Blog
  • Free Essays
  • New Essays
  • Essays
  • Miscellaneous
  • The Newest Essay Topics
  • Index samples by all dates
Join us:
Contact Us