Math in Biotechnology field - Assignment Example

23 February Mathematics Assignment You are setting up a PCR reaction and the protocol asks you to add 0.5ng of DNA to the reaction. You have a sample of DNA which is 5mg/ml. Describe your approach to adding the same volume in parts per million to the reaction? Be specific, describe your dilutions, how much you will pipette, etc?From the 5mg/mL take 1ul and add 999ul or top it up to 1000ul, conc.= 5ug/mLFrom the 5ug/ul, take 1ul and add 999ul or top it up to 1000ul, conc.= 5ng/mL5ng/ml = 5ng/1000uL 0.

5ng =100uLBut 1ppm = 1ng/uL, (IUPAC, 1: Myron, 217)Yet our target 0.5ng is in 100uL (0.005ng in 1uL ) Therefore, based on 1ppm = 1ng/uL, target volume 0.5ng = 0.005ppm2- You are setting up a PCR reaction and the protocol asks you to add 0.2ng of DNA to the reaction. You have a sample of DNA which is 3mg/ml. Describe your approach to adding the same volume in parts per million to the reaction? Be specific, describe your dilutions, how much you will pipette, etc?From the 3mg/mL take 1ul and add 999ul or top it up to 1000ul, conc.

= 3ug/mLFrom the 3ug/ul, take 1ul and add 999ul or top it up to 1000ul, conc.= 3ng/mL3ng/ml = 3ng/1000uL 0.3ng =100uL0.2ng = 67uLBut 1ppm = 1ng/uL, (IUPAC, 1: Myron, 217)Yet our target 0.2ng is in 67uL (0.00298ng in 1uL ) Therefore, based on 1ppm = 1ng/uL, target volume 0.2ng/ul = 0.00298ppm3- You are setting up a PCR reaction and the protocol asks you to add 0.9ng of DNA to the reaction. You have a sample of DNA which is 3mg/ml. Describe your approach to adding the same volume in parts per million to the reaction?

Be specific, describe your dilutions, how much you will pipette, etc?From the 3mg/mL take 1ul and add 999ul or top it up to 1000ul, conc.= 3ug/mLFrom the 3ug/ul, take 1ul and add 999ul or top it up to 1000ul, conc.= 3ng/mL3ng/ml = 3ng/1000uL 0.3ng =100ulTherefore 0.9ng = 300ulBut 1ppm = 1ng/uL, (IUPAC, 1: Myron, 217)Yet our target 0.9ng is in 300uL (0.003ng in 1uL ) Therefore, based on 1ppm = 1ng/uL, target volume 0.9ng/ul = 0.003ppm4- You are setting up a PCR reaction and the protocol asks you to add 3.

0ng of DNA to the reaction. You have a sample of DNA which is 8mg/ml. Describe your approach to adding the same volume in parts per million to the reaction? Be specific, describe your dilutions, how much you will pipette, etc?From the 8mg/mL take 1ul and add 999ul or top it up to 1000ul, conc.= 8ug/mLFrom the 8ug/ul, take 1ul and add 999ul or top it up to 1000ul, conc.= 8ng/mL8ng/ml = 8ng/1000uL 8ng is in 1000ulTherefore 3ng is in = 375ulBut 1ppm = 1ng/uL, (IUPAC, 1: Myron, 217)Yet our target 3ng is in 375uL (0.

008ng in 1uL ) Therefore, based on 1ppm = 1ng/uL, target volume 3ng/ul = 0.008ppm5- You are setting up a PCR reaction and the protocol asks you to add 0.6ng of DNA to the reaction. You have a sample of DNA which is 6mg/ml. Describe your approach to adding the same volume in parts per million to the reaction? Be specific, describe your dilutions, how much you will pipette, etc?From the 6mg/mL take 1ul and add 999ul or top it up to 1000ul, conc.= 6ug/mLFrom the 6ug/mL, take 1ul and add 999ul or top it up to 1000ul, conc.

= 6ng/mL6ng/ml = 6ng/1000uL Therefore the 0.6ng represents =100ul0.6ng = 100ulBut 1ppm = 1ng/uL, (IUPAC, 1: Myron, 217)Yet our target 0.6ng is in 100uL (equivalent to 0.006ng in 1uL ) Therefore, based on 1ppm = 1ng/uL, target volume 0.6ng/ul = 0.006ppmWorks citedIUPAC, Compendium of Chemical Terminology, 997. Web. 22 Feb. 2015Kaufman, Myron. Principles of thermodynamics. New York: CRC Press, 2002. Print.