Thin-Layer Chromatographic Analysis - Lab Report Example

2) Aspirin has the ability to lower the possibility of cancer. According to a recent research, patients who use aspirin are 38% less affected by this disease. Also, using aspirin, it was possible to lower mortality from cancer by 15% and make metastasis less common (Clayden et al. 300). Undoubtedly, aspirin is highly versatile and can be used in multiple situations. But also it has side effects which must be considered before treatment with aspirin is undertaken. For example, aspirin lowers the possibility of formation of clotted blood in the body, which leads to lowering the risk of a stroke.

Because of this aspirin cannot be used by people with tendencies to bleed. Also, aspirin cannot be used by pregnant women, because this drug increases the percentage of mortality of infants, stillbirths, prolongs pregnancy and promotes maternal bleeding. It is also important to note that children who recover from chicken pox or flu must never be treated with aspirin. As it is known, this drug significantly promotes Reye’s syndrome if given to children in the described conditions. In these cases, acetaminophren must be prescribed.

On the diagram above a solvent that will be used in separation is placed inside a TLC vessel. Usually, no more than 1 cm thick is necessary. In order to prevent solvent evaporation, the vessel is closed with a lid. A TLC plate with two spots is placed inside the vessel. The first spot (Black) is a known compound; the second is unknown (White). Initially, both compounds start at the same level (a) and the solvent pushes both compounds up. Gradually, the plate becomes wet with the solvent. The plate should be removed once the solvent reaches the level (b), dried and placed under UV.

If both spots travelled the same distance, then the compounds were identical; and they are different if the travel distances are different. Capillary force is a well-known force that moves the solvent up the TLC plate. During this movement the