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Successful Transformation of Bacterial Cells with Different Plasmid - Lab Report Example

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Bacterial transformation is one the best strategies available in genetic engineering. Moreover, the reports sought to assess the differences between two plasmid models used in studying bacterial resistance (pUC18 for ampicillin and pBR322 for tetracycline)…
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Successful Transformation of Bacterial Cells with Different Plasmid
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Download file to see previous pages These showed that the two models differed in the way competent cells incorporated the plasmids (p<0.05). Perhaps, heat shocking may not be the best method for transforming cells used in the pBR322 model. An alternative approach to the use of chemicals could have validated this comparison. Controls did not report any growth, which validated the fact that the experiment followed the recommended procedure that avoided contamination.
Recent advances in biotechnology have made it possible to isolate cells, carry out cell transformation, identify target-transformed cells, and manipulate other scientific downstream activities using such cells. Molecular medicine depends on novel studies that aim at coming up with novel drugs especially given that most pathogens are developing resistance to the current drugs. Bacterial transformation using E.coli is one of the most studied ways to assess how bacteria develop resistance to antibiotics (Hariharan et al., 2015). In most cases, a plasmid is used. A plasmid is an extrachromosomal DNA molecule that has the potential to replicate autonomously of the chromosomal DNA (Nikaido & Pages, 2012). The plasmid enters a competent E.coli cell and proceeds with multiplication by increasing in number independent of the bacterial chromosome (Yoshida & Sato, 2009; Khademi et al., 2015). The bacterial chromosome only provides the environment and optimal conditions for it to survive (Piddock, 2014). However, some extra chromosomes may incorporate into the genome of the bacterial and thereby depending on the replication of the chromosomal DNA for it to replace (Silver, 2011). Plasmids help in the manipulation of genes at a laboratory level. Several models are used in studying the antibiotic resistance. For instance, pUC18 is a plasmid model used in studying bacterial resistance to ampicillin while pBR322 is used in studying bacterial resistance to tetracycline (Piddock, 2006).
There are three processes of transforming a bacterial cell; these are transformation, transduction, and conjugation. Transduction occurs when a virus infects a bacterial cell and injects a plasmid. ...Download file to see next pagesRead More
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