Successful Transformation of Bacterial Cells with Different Plasmid - Lab Report Example

Assessment of the successful Transformation of Bacterial Cells with Different Plasmid ABSTRACT Bacterial transformation is one the best strategies available in genetic engineering. The report sought to assess the differences between two plasmid models used in studying bacterial resistance (pUC18 for ampicillin and pBR322 for tetracycline). Bacterial cells were aseptically prepared and transformed by heat shocking, and plated on sets of media with different antibiotics alongside control groups and incubated for colony growth. Results showed that pUC18 for ampicillin reported 172 colonies against 35 colonies counted in the pBR322 for the tetracycline model. These showed that the two models differed in the way competent cells incorporated the plasmids (p Read More