The importance of SOC broth for bacterial transformation - Article Example

The importance of SOC broth for bacterial transformation: SOC is acronym for Super Optimal broth with Catabolite repression. It is a rich media and provides good ground for transformation due to the possession of elements by it. It has great importance towards the grown of bacteria and its subsequent transformation. The forefront reason for the importance of SOC broth is that of its ability to provide transformation efficiency. This transformation efficiency pertains to containing of certain Magnesium, Calcium and Potassium ions.

SOC enables the transformation of bacteria and overall yield in this regard. Various media serve as element of growth towards bacteria. The elementary one of it is Lysogeny Broth. SOC is associated with it (Hannan).SOC is a variant and a further refined form of SOB. While SOB is devoid of glucose, it is the presence of glucose to SOB which makes it a SOC. It also helps identifying and determining different colonies of bacteria, their growth time, and their overall nomenclature.SOC media is enriched with glucose which makes it more sought after for the formation and lateral transformation of bacteria.

However the presence of bacteria is a concern since it strengthens the overall cell existence and ultimately more resistance.Other advantage to SOC broth is its adjustment to the PH level around 7, which is most desired and provides options towards a neutral usage. Apart from this, SOC contains certain percentage and proportion of MgSo4. Hence all these traits and possessions make SOC broth more suitable for bacterial transformation than the rest.References:D., Hanahan. Why is SOC medium recommended for transformations? 2013. 14 March 2013 .

Kahl, Guenter and Khalid Meksem. The Handbook of Plant Mutation Screening. John Wiley & Sons, 2009.