Assay of protein turnover using a bioluminescent reporter - Essay Example

Comments (0) Cite this document
For the sample LUCWT, light emitted in 10 seconds per ul (RLU/ul) at time zero was 1236.8 RLU/ul. The average protein concentration at time zero was 0.53 ug/ul while the Relative Luciferase Activity at…
Download full paperFile format: .doc, available for editing
GRAB THE BEST PAPER92.2% of users find it useful
Assay of protein turnover using a bioluminescent reporter
Read TextPreview

Extract of sample "Assay of protein turnover using a bioluminescent reporter"

Assay of protein turnover using a bioluminescent reporter Affiliation Results and Discussion Answer to Question The results were relatively different for the corresponding LUCWTs and LUCPs. For the sample LUCWT, light emitted in 10 seconds per ul (RLU/ul) at time zero was 1236.8 RLU/ul. The average protein concentration at time zero was 0.53 ug/ul while the Relative Luciferase Activity at time zero was 2333.58. For the sample LUCP, light emitted in 10 seconds per ul (RLU/ul) at time zero was 1205.5 RLU/ul. The average protein concentration at time zero was 0.53 ug/ul while the Relative Luciferase Activity at time zero was 2274.47. The difference could have been caused by various factors including pipetting errors (Gould & Subramani, 1988).
2. Answer to Question 2
An estimate of a half-life for the LUCP enzyme is (How to Calculate Half Life, 2015):
Half-life = t*In 2
10seconds = t*0.301
t=10seconds*0.301 =30.1 seconds.
3. Answer to Question 3
It is important for some proteins to have a short half-life but no others like clusterin because they have to control excessive growth of cells. Clusterin in this case controls prostate cancer cells (Rizzi, Caccamo, Belloni, & Bettuzzi, 2009).
4. Answer to Question 4
In designing an experiment using MetLUC to assay the efficacy of three compounds with respect to BF A, the first thing to consider is that the activity of BF A is known. The BF A would thus be used as the control variable (Schultz, Cegielski, & Hastings, 2005). Analysis of each of the three novel compounds would be done. This would be based on their respective signal sequence, in which each of them would be expressed in tissue culture cess. Comparison of how the MetLUC protein for each compound would be translocated to the ER and the resulting secretion into the medium in which the cells are growing would be done. The results of each would be compared to the known facts about the same experiment in BF A.
Gould, S., & Subramani, S. (1988). Firefly luciferase as a tool in molecular and cell biology". . Anal. Biochem. 175 (1), 5–13.
How to Calculate Half Life. (2015). Retrieved from wikihow:
Rizzi, F., Caccamo, A., Belloni, L., & Bettuzzi, S. (2009). Clusterin is a short half-life, poly-ubiquitinated protein, which controls the fate of prostate cancer cells. Journal of Cell Physioly, 19(2), 14-23.
Schultz, L. L., Cegielski, M., & Hastings, J. (2005). Crystal structure of a pH-regulated luciferase catalyzing the bioluminescent oxidation of an open tetrapyrrole . Proc. Natl. Acad. Sci. U.S.A., 102 (5), 1378–83. Read More
Cite this document
  • APA
  • MLA
(“Assay of protein turnover using a bioluminescent reporter Essay”, n.d.)
Retrieved from
(Assay of Protein Turnover Using a Bioluminescent Reporter Essay)
“Assay of Protein Turnover Using a Bioluminescent Reporter Essay”, n.d.
  • Cited: 0 times
Comments (0)
Click to create a comment or rate a document

CHECK THESE SAMPLES OF Assay of protein turnover using a bioluminescent reporter

Protein purification using anion exchange membranes

...with the charged groups on the surface of the immobile phase, an anion exchange membrane in this case. The charges on the protein and the membrane must be opposite for the exchange interaction to occur. The support membrane, which has covalently attached positive functional groups, is referred to as an anion exchanger if mobile negatively charged anions will be the exchanged species. Conversely, an exchanger with covalently bound negative groups is a cation exchanger, the mobile counter ions being cations. If an anion exchanger is being used, a negatively charged molecule will bind more avidly if the pH is raised (1). Column chromatography methods are robust and reliable but generally have low throughput...
4 Pages(1000 words)Term Paper


...Graph Calibration curve The calibration curve is found to be good in terms of its statistical significance, as having R2 value of 0.94.And can be used as standard curve for Rabbit Ig G. There is slightly deflection at concentration of 2.0 this might be due to pipetting error as points before and after of it follows in line. It is important to use multichannel pipette to avoid any manual error at a time of filling reagents to microtiter plates. Similarly, to avoid error like at concentration of 2.0 there must be at least 3 replicates which make data more meaning full and realistic in terms of statistic. Graph 2: Monoclonal mouse anti-rabbit Ig G In this graph various dilution of mouse anti...
2 Pages(500 words)Essay


...TURNOVER CASE The of Mellencamp is a non-profit private academic It is located in Northern Luzerne County in Pennsylvania.Four colleges function under this institution. Let us consider the various aspects of the turnover cost of the institution to assess its real standpoint. Cost To The University Each Year Assumption: The working hours for an employee of the University of Mellencamp are 8 hours per day and the University works 5 days per week. The University of Mellencamp employs 3 types of Human Resources (1) Faculty (2) Administration (Management) (3) Support staff Cost of Faculty: (1) Present Faculty = 120 (2) Posts vacant =10 (3) Total strength of faculty = 130 (4) Working hours in a week = 40 (5) Therefore, working hours... in a...
4 Pages(1000 words)Research Paper


...Community Pharmacy Placement Exercise Physical Environment The pharmacy that I have chosen for this exercise is the Campbell Pharmacy. Campbell Pharmacy is located in North Canberra, in the suburbs of Campbell. The pharmacy is surrounded by many small shops and businesses servicing the people living in the area. There is a supermarket in the area, along with several restaurants and food establishments, salons, newspaper stands, and a laundry shop. The people most frequently patronize the supermarket and the food establishments in the area. The nearest medical centre, which is the Campbell Medical Centre is located very near the pharmacy; and the Campbell Nursing Home is also located very near the pharmacy. None of the nearby... Pharmacy...
2 Pages(500 words)Essay


...felt that she had a bit of hay fever. She asked to be given Phenergan as an antihistamine. I observed that the pharmacist asked her if she was going to drive soon after taking the medication, and if she was taking any other medications at that time. She said she was not going to drive, but was just not feeling well. After the interview, the pharmacist recommended that the patient take Claratyne instead of Phenergan because Claratyne had a non-sedating property and is used to relieve allergies. I reviewed my knowledge of the drug and was able to explain further to the patient that Claratyne is more appropriate for her because it does not enter the central nervous system, therefore does not make the patient drowsy and...
2 Pages(500 words)Essay


...greatly impacts on the pharmacy profession because it helps the pharmacist be better informed about what he can and cannot do under the law. The Public Health Act of 1997 sets the laws for the protection of public health and the prevention of diseases. It defines and describes the health authorities tasked with safeguarding the health of the people. It also defines unsanitary conditions and public health risks and procedures for handling said risks. The Public Regulations Act of 2000 is the regulation that prescribes methods for communicable disease control like vaccinations and immunizations; it also prescribes for methods in cancer detection and reporting, general sanitation, and drug preparation. The act and...
2 Pages(500 words)Essay


...Report Layout Summary Introduction Materials and Methods Results Discussion Future analysis Conclusion References Page No. 3 4 13 16 18 18 19 20 Purification of bovine lactoferricin from cow’s milk Summary Bovine lactoferrin from cow’s milk was hydrolysed using porcine pepsin. The peptic digest was subjected to chromatography on the weak cation-exchange resin, Macro-prep CM. Two protein fractions F1 and F2 corresponding to two eluate peaks were collected using 10% acetic acid as the eluent. The fractions,...
25 Pages(6250 words)Research Paper

Determination of Protein using Biuret Reagent

...for the purpose of clarity. In many experiments, biuret can be scaled using smaller sized cuvette, which consumes less protein. Proteins with abnormal low or high percentage of amino acids and aromatic side groups always gives low or high readings, respectively (Robinson, and Hogden, 1940, 32) In the case of bovine serum, albumin will obviously develop a linear relationship between the amount of protein and absorbance over a range of 0.5 to 20mg protein. However, analysis show assay below 0.5 mg is not reliable. In other cases, the upper limit may increase depending with the reagents. A straight line graph is achieved after plotting the...
3 Pages(750 words)Lab Report

Investigative Reporter Assigment

... of the Journalism, Mass Media and Communication of the Concerned 16 November Iraqi Weapons of Mass Destruction- An Investigation There is no denying the fact that the justification of the invasion of Iraq was to a great extent largely based on the oft repeated and bolstered allied premise that not only Iraq had amassed a significant stock of the weapons of mass destruction, but, Iraq also intended to use this weapons of mass destruction against neighbours to whom it was averse to, and also aspired to unleash these weapons on coveted Western enemies, if the need be. The intelligence agencies of most of the allied nations furnished detailed reports ascribing to Iraq the possession of weapons of mass destruction and the governments... of...
7 Pages(1750 words)Research Paper


...Protein Q Describe the alanine-glucose cycle. The alanine-glucose cycle is responsible for the exchange of alanine molecule between skeletal muscles and liver. Like glutamine, alanine is a very important resultant of amino acid degradation that is used in the body. Alanine is the product of transamination of pyruvate that happens in the muscles. From the muscles, the alanine is absorbed in the liver where the process of transamination occurs. The process occurs in the presence of alpha-ketoglutarate. This cycle generates pyruvate which enters the gluconeogenesis cycle and glutamate which enters the urea cycle. The enzyme which is required for these processes to occur is alanine amino transferase. Q 2....
2 Pages(500 words)Essay
sponsored ads
We use cookies to create the best experience for you. Keep on browsing if you are OK with that, or find out how to manage cookies.

Let us find you another Essay on topic Assay of protein turnover using a bioluminescent reporter for FREE!

Contact Us