Nobody downloaded yet

Size Exclusion Chromatography- Protein Separation Polyacrylamide Gel Electrophoresis - Lab Report Example

Comments (0) Cite this document
Summary
The absorbance of each fraction at 200nm was determined and plotted as shown in figure 1 below. Even though the resolution between the…
Download full paperFile format: .doc, available for editing
GRAB THE BEST PAPER94.6% of users find it useful
Size Exclusion Chromatography- Protein Separation Polyacrylamide Gel Electrophoresis
Read TextPreview

Extract of sample
"Size Exclusion Chromatography- Protein Separation Polyacrylamide Gel Electrophoresis"

Download file to see previous pages These standards were used to plot the calibration curve.
The retention times, which is equivalent to elution volume (Ve) of each protein was plotted as a function of the log 10 of the molecular weight of the standards as shown in figure two. This calibration curve was used to estimate the relative molecular weights of the unknown samples.
The Value for X and Y in this function is equivalent to elution volume (Ve) and Log10 of the molecular weight. Substituting Ve with the retention time value, the Log M and molecular weights of the unknown samples were determined as shown on table three below.
Size exclusion chromatography is a HPLC technique that separates molecules based on their size or molecular weight (Bernd, 2). Using this technique the molecular weight of five unknown protein molecules was estimated as shown in table three. However, the validation assay of the technique did not give expected resolution, limits the validity of the results. Clear and distinct peaks on this graph depict a high resolution of the assay and thus credible results. A number of factors; such as column length, flow rate and technical errors may have influenced the poor resolution of the peaks in the UV protein absorption profile (Hong and Fountain, 5-6). Further optimization experiments may be necessary to overcome these errors and achieve a good ...Download file to see next pagesRead More
Cite this document
  • APA
  • MLA
  • CHICAGO
(“Size Exclusion Chromatography- Protein Separation Polyacrylamide Gel Lab Report”, n.d.)
Retrieved from https://studentshare.org/chemistry/1640313-size-exclusion-chromatography-protein-separation-polyacrylamide-gel-electrophoresis
(Size Exclusion Chromatography- Protein Separation Polyacrylamide Gel Lab Report)
https://studentshare.org/chemistry/1640313-size-exclusion-chromatography-protein-separation-polyacrylamide-gel-electrophoresis.
“Size Exclusion Chromatography- Protein Separation Polyacrylamide Gel Lab Report”, n.d. https://studentshare.org/chemistry/1640313-size-exclusion-chromatography-protein-separation-polyacrylamide-gel-electrophoresis.
  • Cited: 0 times
Comments (0)
Click to create a comment or rate a document
CHECK THESE SAMPLES - THEY ALSO FIT YOUR TOPIC
Agarose Gel Electrophoresis of DNA
...? Agarose Gel Electrophoresis of DNA DNA is the double stranded helical structure which carries the genetic information. The study of the DNA molecule will give us the details about life. DNA molecules can be extracted from the cell using the extraction techniques and they are then quantified using the agarose gel electrophoresis. (Westermeier 2006). Agarose gel electrophoresis is the most common and easiest technique to separate the DNA fragments. Agarose is a polymer that forms helical linking strands between the molecules and they are held together by hydrogen bonds. Based on the concentration of the agarose in the gel, the pore size also varies. The DNA fragment is separated in the Agarose based on the pore size... (that is the...
5 Pages(1250 words)Lab Report
Agarose Gel Electrophoresis of DNA
...Agarose Gel Electrophoresis of DNA Introduction Electrophoresis refers to a method used toseparate and purify macromolecules, mostly nucleic acids and proteins, which differ in conformation, size, or charge. It is among the widely used techniques in molecular biology and biochemistry. Gel electrophoresis refers to the method of analysis and separation of macromolecules (proteins, RNA, and DNA) and their respective fragments, depending on their charge and size. Gel electrophoresis is applied in clinical chemistry to...
2 Pages(500 words)Term Paper
Fast protein liquid chromatography (FPLC)
...chromatography 7) Expanded bed adsorption chromatography 8) Size exclusion chromatography 9) Ion exchange chromatography 10) Supercritical fluid chromatography 11) FPLC The FPLC is the method of interest in this case. The FPLC method was developed and marketed in Sweden by the Pharmacia Company in 1982. It was originally called fast performance liquid chromatography. Principle of functioning The purpose of purifying proteins with FPLC is to deliver quantities of the target protein at sufficient purity. This is done in a way that ensures the protein is in...
7 Pages(1750 words)Term Paper
Protein Extraction and Gel Electrophoresis
... Proteomics and Gel Electrophoresis Abstract Proteomic is generally defined as the direct analysis of proteins in terms of their presence and relative abundance. Gel electrophoresis is a significant methodology employed for extraction of proteins in proteome analysis. The following is a report on an experiment conducted to determine the concentration of proteins in two samples using the Bradford assay and separating the proteins using sodium dodecyl sulfate-polyacrylamide gel electrophoresis. It involved three steps, that is,...
5 Pages(1250 words)Lab Report
Conventional PCR using agarose gel electrophoresis detection
...Conventional PCR using agarose gel electrophoresis detection According to Lewis “Agarose gel electrophoresis is the easiest and commonest way of separating and analyzing DNA”. Conventional techniques used to detect mycoplasma involve culturing samples on selective media, which needs at least a week to obtain the results (HD BIOSCIENCES Co., LTD 2). The reason to add gel in this method is to either quantify the DNA or separate the particular band of DNA. With the addition of edhidium bromide, DNA becomes visible. This binds strongly to DNA by intercalating between the bases and is fluorescent meaning that it absorbs invisible UV light and transmits the energy as visible orange light (Lewis). While addition of gel, the care... for the...
2 Pages(500 words)Essay
Chromatography
...SEPARATING AND IDENTIFYING FOOD DYES BY PAPER CHROMATOGRAPHY The resolution of blue green 3, yellow 5, yellow 6, red 40, blue 2, and red 3, determined through the differences in Rf values, was best achieved by paper chromatography using 0.10% NaCl solution, instead of water or 70% isopropyl alcohol as mobile phase. Difference between the polarity of the solutions relative to the stationary phase caused the observed differences among the chromatograms obtained using the different solvents. On the other hand, the relative polarity of the dyes resulted to differences in their interactions with the phases, and subsequently in their distances traveled along the chromatography...
6 Pages(1500 words)Lab Report
How to make and run an agarose gel (DNA Electrophoresis)
...Agarose Gel Electrophoresis of DNA For isolation of genomic DNA, agarose gel electrophoresis is done which is based on the principle that on disruption and lysis of cell components, followed by removal of proteins and sugar, nucleic acids can be recovered which can be observed through a UV transilluminator on the agarose gel due to prior staining by ethidium bromide. Materials: Agarose powder, Electrophoresis buffer (TAE), gel casting tray, Ethidium bromide, Comb, Sample, Loading buffer, UV transilluminator. Method: Agarose solution (generally .8%) is prepared for isolation of DNA. However, the...
1 Pages(250 words)Article
ELECTROPHORESIS
...not be related to the quantity of protein. This may be because the uptake of the stain may happen in an irregular fashion. Analytes that can be separated by ElectrophoresisProteins and nucleic acids are some of a common analytes that are separated by electrophoresis. Electrophoresis of ProteinsProteins are amphoteric in nature and can possess an overall negative or positive charge depending on the pH of the solution. Sodium dodecyl sulphate-polyacrylamide gel electrophoresis (SDS-PAGE) is the commonly employed technique for...
6 Pages(1500 words)Essay
Distillation and Gas Chromatography: Separation of Hexanes and Toluene
...semimicroscale distillation is an appropriate method for the separation of volatile hydrocarbons such as toluene and hexane. Distillation and Gas Chromatography: Separation of Hexanes and Toluene Introduction According to Pavia et al., (56), the separation and purification of two liquids from a mixture requires the utilization of a method called distillation. The problem in the separation of two liquids is how to separate them. Hence, in the experiment two volatile liquids, namely; toluene and hexane shall be separated using fractional semimicroscale distillation. In this experiment, the two liquids, hexane and toluene,...
4 Pages(1000 words)Lab Report
Protein
...Protein Q Describe the alanine-glucose cycle. The alanine-glucose cycle is responsible for the exchange of alanine molecule between skeletal muscles and liver. Like glutamine, alanine is a very important resultant of amino acid degradation that is used in the body. Alanine is the product of transamination of pyruvate that happens in the muscles. From the muscles, the alanine is absorbed in the liver where the process of transamination occurs. The process occurs in the presence of alpha-ketoglutarate. This cycle generates pyruvate which enters the gluconeogenesis cycle and glutamate which enters the urea cycle. The enzyme which is required for these processes to occur is alanine amino transferase. Q 2. List as many...
2 Pages(500 words)Essay
sponsored ads
We use cookies to create the best experience for you. Keep on browsing if you are OK with that, or find out how to manage cookies.
Let us find you another Lab Report on topic Size Exclusion Chromatography- Protein Separation Polyacrylamide Gel Electrophoresis for FREE!
logo footer
Contact us:
+16312120006
Contact Us Now
FREE Mobile Apps:
  • StudentShare App Store
  • StudentShare Google play
  • About StudentShare
  • Testimonials
  • FAQ
  • Blog
  • Free Essays
  • New Essays
  • Essays
  • Miscellaneous
  • The Newest Essay Topics
  • Index samples by all dates
Join us:
Contact Us