quora
Nobody downloaded yet

SDS PAGE - Term Paper Example

Comments (0) Cite this document
Summary
First-name Last Number 9 April 2013 SDS PAGE Background The method of separation of macromolecules in an electric field is called electrophoresis. Sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS PAGE) is a method of separating protein molecules based on their ability to move through an electric field depending on size of their polypeptide chains and molecular weight…
Download full paperFile format: .doc, available for editing
GRAB THE BEST PAPER93.5% of users find it useful
SDS PAGE
Read TextPreview

Extract of sample
"SDS PAGE"

Download file to see previous pages -mecaptoethamol prevents oxidation of cysteines and break up disulphide bonds, Bromophenyl dye is used to help in visualizing the sample as it travels across the gel and glycerol is added to make the sample fall to the bottom. (Leamlli, 1970). Electric charge is applied across the gel to influence the movement of protein molecules to positively charged end. Principal behind SDS PAGE The structure of protein molecules to be separated has portions of negative and positive charges resulting from charged R-groups of particular amino acid and hydrophobic portions of nonpolar R-groups hence difference in shapes and sizes of protein molecules. (Hempelmann, 2008) Therefore, the first stage of SDS PAGE is to make the protein molecules linear and to ensure that there are no secondary, tertiary, or quaternary molecular structures in the sample. This is achieved by injecting the sample with SDS, which is a detergent that can dissolve the membrane and solubilise the protein molecules. Its negative charge overcome positive charges of protein molecules. The resulting protein is denatured, linearized, and negatively charged. The next stage in SDS PAGE is the separation process. This is based on the molecular size and weight of the molecules, sieving properties of the gel is of great assistance at this stage since protein molecules have the same charge-to-mass-ratio. The gel is not a solid but made up of series of tunnel of different diameters running from one end to the other and are scattered through the gel. (SDS PAGE, 2009) Velocity of particles moving through an electric field is directly proportional to strength of the electric field and degree of charge in the particle but inversely proportional to size of the particles and viscosity of the medium. This is the basis upon which protein of different sizes are separated. (SDS PAGE. 2009). The discontinuous pH parts of the gel come handy in aligning the proteins properly at the starting point. Laemmli gel is composed of stacker and running gel, the running gel is buffed with Tris to pH of 8.8 with HCl; stacker gel is adjusted with Tris to pH 6.8 with HCl. The electrode buffer is adjusted to pH 8.3 using glycine and the gel is then run at a constant voltage. (Leamlli, 1970) When the power is switched on, glycine (which is a weak acid and can exist in an uncharged state as zwitterions at low pH, or in a charged state as glycinate anion at high pH) ions in the running buffer wants to move away from the negative electrode towards the sample and the stacking gel. The pH in stacking gel is low and so glycin ions lose their charge and slow down. In the stacker and sample, negatively charged mobile chloride ions move away from the cathode creating a narrow zone of very low conductance (very high electrical resistance) in the top of the stacking gel. Almost all the applied voltage is concentrated in this small zone. The very high field strength makes the negatively charged proteins to move forward. The trick, however, is that they can never outrun the chloride ions. If they did, they would find themselves in a region of high conductance and very low field strength and would immediately slow down. The result is that all the proteins move through the stacker in a tight ...Download file to see next pagesRead More
Cite this document
  • APA
  • MLA
  • CHICAGO
(“SDS PAGE Term Paper Example | Topics and Well Written Essays - 750 words”, n.d.)
Retrieved from https://studentshare.org/biology/1473154-sds-page
(SDS PAGE Term Paper Example | Topics and Well Written Essays - 750 Words)
https://studentshare.org/biology/1473154-sds-page.
“SDS PAGE Term Paper Example | Topics and Well Written Essays - 750 Words”, n.d. https://studentshare.org/biology/1473154-sds-page.
  • Cited: 0 times
Comments (0)
Click to create a comment or rate a document
CHECK THESE SAMPLES - THEY ALSO FIT YOUR TOPIC
The topic for your paper will be Baze v Rees, a Supreme Court hearing on lethal injection. The question for your 3-4 page paper
The main claims by the petitioners were that the three components used in the injection may be injected in an improper way causing Baze and Bowling a severe pain. This is why the case was granted by certiorari. Nevertheless, all points concerning the 8th Amendment are to be reconsidered so as to find out the truth through the analysis of both sides in the case.
3 Pages(750 words)Term Paper
Craft a 6 -page paper referencing the following Research hypotheses See Details
This study is, therefore, comprised of two parts. The first part of the study identifies a sample population of females between the ages of 20 and 25, who experienced traumatic episodes of bullying between the ages of 9 and 12. In this part of the study, the females will participate in a survey in which they are asked to recall their most traumatic bullying experiences at this much earlier age.
6 Pages(1500 words)Term Paper
*The student will develop a 10-12 page term paper that answers Yali's question. The production of this paper carries the signifi
Yali classified the European imported products as cargo. The cargoes included soft drinks, steel axes, matches, medicines, clothes, and umbrellas (Diamond 7). The question verged on the differences between the power and technology advances between the European people who arrived in New Guinea and the New Guinea population.
10 Pages(2500 words)Term Paper
Course Paper Assignment. You are required to write a 5-7 page paper on a concept investigating the social effects of aviation an
Of all means of transportation, aviation is the fastest growing, thanks to the high speeds and low operating costs of jet airliners. The annual global growth rates of aviation (total number of kilometers flown by all passengers) were approximately 10% in the 1960s and were approximately 5%-7% in the 1990s (Whitelegg, 2000).
5 Pages(1250 words)Term Paper
Performance Review Takes a Page from Facebook
There is a great deal of merit to this statement. According to Fairholm (2009), the ability to develop a cohesive and dedicated organizational culture involves establishing regular communications systems with internal stakeholders. In human resources, one of the most important constituent parts of maintaining competitive advantage is human capital resource.
4 Pages(1000 words)Term Paper
Current event synopsis 2 page
ticle, “After Debit Card Fraud, a Bank Feels Customers’ Frustration” by Ron Lieber, explores the impact of fraudulent transactions on customer welfare and/or satisfaction
2 Pages(500 words)Term Paper
Creative thinking:Choose one pattern of change in thinking. In a 2-3 page paper in APA format, discuss how you personally are going to work to make this change in your practice environment
It is necessary for nurses to understand that going for nothing less than perfect in them or others will only lead to emotional distress in both personal and professional relationships. This paper will
2 Pages(500 words)Term Paper
A movement in American history
Movements have flourished under democratized countries. Sociological theories of social movements have all come to a conclusion that social movements address matters, such
3 Pages(750 words)Term Paper
10 page Term Paper of an amendment to the United States Constitution
One of the central doctrines on which the constitution is created is the separation of powers, which separates the power between three branches of
10 Pages(2500 words)Term Paper
Students will complete an 8-page paper (including title and reference pages) providing a critical look at the coverage of a social problem in the news media
Egypt is situated in the south west part whereas Israel is situated in the eastern part of this city. The conflict between Palestine and Israel was started in the mid 20th century for the right of governance in Gaza. In
6 Pages(1500 words)Term Paper
sponsored ads
We use cookies to create the best experience for you. Keep on browsing if you are OK with that, or find out how to manage cookies.
Let us find you another Term Paper on topic SDS PAGE for FREE!
logo footer
Contact us:
+16312120006
Contact Us Now
FREE Mobile Apps:
  • StudentShare App Store
  • StudentShare Google play
  • About StudentShare
  • Testimonials
  • FAQ
  • Blog
  • Free Essays
  • New Essays
  • Essays
  • Miscellaneous
  • The Newest Essay Topics
  • Index samples by all dates
Join us:
Contact Us