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Mammalian cell - Essay Example

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Reduction of ATP hydrolysis activity of vacuolar-type ATPase/synthase (V0V1) as a result of ADP inhibition occurs as part of the normal mechanism of V0V1 ofThermus thermophilus but not V0V1 of Enterococcus hirae or eukaryotes (Hu, Wei-Shou, Zeng, & Berger 13). To investigate the…
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Download file to see previous pages hirae V1 is central to increasing binding affinity of the chimeric V1 for phosphate, resulting in reduction of the ADP inhibition. These findings together with a comparison of the crystal structures of T. thermophilus V1 with E. hirae V1 strongly suggest that the A subunit adopts a conformation in T. thermophilus V1 different from that in E. hirae V1. This key difference results in ADP inhibition of T. thermophilus V1 by abolishing the binding affinity for phosphate during ATP hydrolysis (Hu, Wei-Shou, Zeng, & Berger 13).
The preparation of Chimeric was constructed by an overlap of PCR-based method. The second procedure, on the other hand involved the introduction of cysteine residues into the D subunit contained in the V1 (Hu, Wei-Shou, Zeng, & Berger 13). Lastly, the last portion of the experiment involved the introduction of protein concentrations of the already purified V1constructs which were established from the UV absorbance which was calibrated by the quantitative amino acid examination (Mather, ...Download file to see next pagesRead More
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