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PANC-1 cell line - Essay Example

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The illness has a survival rate of about five years. A phenomena referred to as the Warburg effect shows that the cells of cancer display enhanced glucose that is converted to lactate and…
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PANC-1 cell line
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Download file to see previous pages nts who go through the resection and have the free margins of tumor, the reported 5-year rate of survival can only be between ten to twenty five percent (Ke, Wang, Xu, and Abassi, 2011). Whenever the pancreatic cancer turns to be metastatic, it becomes uniformly fatal having an overall survival of approximately six months from the time of diagnosis (Blackburn, Vay Liang, and Milner, 2011). For the past thirty years a combined method of radiotherapy, surgery, and chemotherapy have been applied in treating pancreatic cancer (Mani, Guo, & Liao, 2008). However, there has been no huge improvement in the rate of survival. This means that a powerful therapeutic method is required. In attempts of obtaining an appropriate therapeutic strategy for pancreatic cancer, this paper investigates the effect of caffeine on the PANC-1 cells.
Pancreatic Cancer is one of the leading causes of deaths in world. Even with recent efforts to come up with modalities, the rate of mortality remains being high. Caffeine can be used in treating this illness, even though the molecular mechanisms of the agents are not understood fully. This is, to some extent, responsible for the failing of these agents in treating pancreatic cancer. In a study conducted in this field, PANC –1 mutant p53 was used in investigating the influence of caffeine on the growth of cells and the effect on cell modulation cycle and the gene apoptosis relation (Rückert, Werner, & Aust, 2012). The extraction of proteins from these cells was treated 4 mM of Caffeine was put to a western blot analysis. The cells of drug treating were analyzed for the calculation of the number of those cells that experience apoptosis. In the observation, the study found out the time and dose dependent inhibition of growth was seen in the PANC cells after the treatment with caffeine (Mani, Guo, & Liao, 2008). The analysis by the western blot displayed an up regulation of the p21WAF1 in the cell lines that were treated with caffeine. In ...Download file to see next pagesRead More
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