Assignment-Synthesis of a Protein - Lab Report Example

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The beginning sequence can be identified using the start codon. The start codon is typically AUG in eukaryotes (or ATG in DNA; this also encodes methionine). In addition to AUG, alternative start codons, mainly GUG and UUG are used in prokaryotes. The yellow highlighted ones…
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Assignment-Synthesis of a Protein
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Download file to see previous pages Errors in protein synthesis produce polypeptides displaying a gain of toxic function which may confer an alternate or pathological function on a normal, folded protein. In case of enzymes, the hydrophobic core provides structural stability for the molecule and amino acid changes may result in unstable protein product that is temperature sensitive. As the catalytic site of the enzyme is extremely sensitive, a single point mutation (either a deletion or insertion or substitution) may completely abolish function.
5. The above sequences represent those of eukaryotes as the intron or the non-coding region (codons from 24 to 66, including 66) had to be removed before translating it into a polypeptide or a protein.
Whether sequence is eukaryotic or prokaryotic, it can be identified by the presence of introns and exons. In the case of prokaryotes, the initial RNA molecule or initial transcript is equivalent to the final mature RNA. In most eukaryotic genes, the initial transcript is processed so that the mature RNA is different. Most eukaryotes have genes that contain introns that do not code for polypeptides. However, prokaryotic genes do not contain introns. Eukaryotic RNAs, after transcription, have the intron sequences that are removed/ spliced in order to produce the final mature RNA. The sequences in eukaryotes that are represented in the final RNA and code for amino acids in a polypeptide are called exons.
6. The tRNA anticodon sequence that would build this protein is complementary to the mRNA sequence. The tRNA anticodon bonds to a given mRNA codon, by hydrogen bonding between A-U pairs and C-G pairs.
The above sequence represents the intron or the non-protein-coding region of the initial mRNA transcript. The intron sequences are spliced in order to produce the final mature RNA in eukaryotes. Introns play important roles in gene regulation. One of the functions of introns is that they ...Download file to see next pagesRead More
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