Viral Genome Detection Using Polymerase Chain Reaction - Lab Report Example

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The purpose of this experiment “Viral Genome Detection Using Polymerase Chain Reaction” is to detect the presence of viral genome in the genomic DNA of HeLa and MRC5 cell lines. Human papillomavirus (HPV) 18-specific primers were used to amplify viral DNA…
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Viral Genome Detection Using Polymerase Chain Reaction
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Download file to see previous pages The emergence of molecular diagnostic tools has circumvented and altered the limitations brought about by conventional diagnostic techniques (Lion et al., 2006). Specifically, genome-based techniques are increasingly becoming popular due to their high specificity and sensitivity. One tool that has revolutionized the field of clinical diagnostics is polymerase chain reaction (PCR), an enzymatic procedure which amplifies a specific fragment of DNA or RNA (Lion et al., 2006). Since PCR makes use of nucleic acids to detect the presence of organisms, it is extremely useful in the identification of notoriously difficult-to-detect pathogens like viruses.
It has been established that human papillomavirus (HPV) infection is a necessary precursor for the onset of cervical cancer (Stanley 2010). In fact, approximately 90% of cervical cancer cases can be accounted for by HPV types 16 and 18. Since HPV infection is very common with a lifetime risk of infection of 50-80%, its accurate diagnosis is very crucial (Stanley 2010).
. The purpose of this experiment is to detect the presence of viral genome using the polymerase chain reaction. Specifically, the experiment seeks to compare the genomic DNA extracted from HeLa cells, a cervical cancer cell line and MRC5 cells derived from a 14-week old fetal lung tissue in order to confirm that HeLa cells contain human papillomavirus (HPV) 18 DNA.  ...Download file to see next pagesRead More
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