Molecular biology cloning and Brachyury gene - Essay Example

Such recombinant vectors imitate effectively in specific strains of bacterial host cells, leading to the production of several copies of the recombinant DNA molecule, which was put into the vector (Eisen, et al, 2002, p 5483; Sambrook, Fritsch & Maniatis, 1989, p 891). 2. Brachyury gene: definition and the relation between the genes with cancer Brachyury refers to a protein which is encoded by the T gene in humans. It is a transcription factor that is within the T-box complex of genes, which has been identified in all bilateral animals.

Brachyury also helps in defining the mesoderm in the process of gastrulation (National cancer institute, nd, para 5). The T gene is first expressed at the ending stage of blastula, at the mesoderm region and then later in the process of gastrulation. Expression of the Brachyury gene is contained to the tail bud and the notochord region. Brachyury is expressed within the endothermal epithelial cells of the hypostome in the diploblastic Hydra that makes up the apical portion of the polyp cranium.

Therefore, the T gene plays an essential role in forming the axis of the initial body. The T gene is critical in the molding tripoblasts’ and diploblasts’ layers of germ, as well as, in the formation of the apical pole (Rolf, 2013, para 1). Genetic evaluations of individuals with hereditary form of bone cancer reveal that acquiring an additional T gene might cause the uncommon bone cancer. A study of seven families diagnosed with chordoma involving two or more blood relatives was conducted by the NCI researchers.

Linkage analysis approach was utilized to explore whether certain genetic markers with established points in humans are present at birth along with chordoma. The initial analysis set out six genome areas that necessitated an extra examination. A comprehensive hereditary examination revealed that chromosome 6 section was more apt to be related with hereditary bone cancer. Brachyury gene that is contained in chromosome 6 is exceptionally significant in the growth of notochord. Results from earlier examinations show that the T genes is found in almost all bone cancer cases, but not in the wide ranging assortment of ordinary body tissues or other malignant cells kinds.

As such, the observation made the Brachyury gene an excellent candidate for involvement in the uncommon bone cancer (Rolf, 2013, para 2) 3. What is plasmid pNEB193? Plasmid pNEB193 is an enigmatic and a fresh plasmid. It was first found in NBRC14147 in S.albulus. Its functions are not known, though the cryptic plasmid pNEB193 replicon was utilized in duplication of vector construction (Sambrook, Fritsch & Maniatis, 1989, p 894). 4. Cloning technique What is the aim from using the polymerase chain reaction PCR in the cloning technique?

Polymerase chain reaction is the primer-meditated enzymatic amplification of distinctively replicated or genomic DNA sequences. The template DNA includes a target sequence that can be very long in terms of nucleotides length. The main aim of using PCR is to clone genes directly without constructing DNA libraries. PCR makes gene cloning rapid by starting with a whole genome, PCR permits the DNA from a chosen region to be increased several billion fold, successfully sanitizing this DNA away from the remainder of the genome (Taylor & Francis group, 2010, p 544).

PCR is mostly employed in molecular biology and