Nobody downloaded yet

Molecular Basis of Disease - Lab Report Example

Comments (0) Cite this document
Name: Course: Tutor: Date: PERNICIOUS ANEMIA Introduction Pernicious anemia is a disease caused by the loss of the parietal cells of the gastric mucosa. These cells produce an essential protein known as intrinsic factor. The intrinsic factor helps in vitamin B12 absorption…
Download full paperFile format: .doc, available for editing
GRAB THE BEST PAPER93.2% of users find it useful
Molecular Basis of Disease
Read TextPreview

Extract of sample
"Molecular Basis of Disease"

Download file to see previous pages People lacking the absorption ability of vitamin B12 remain with the disease for the rest of their lives. Parietal cells found in the gastric cells are responsible for production of hydrochloric acid which is important in digestion as it provides a favorable medium for enzyme reaction. The serum found from patients having this disease react with ? and ? sub units. The antibodies are used for diagnostic purposes. This experiment involves testing of serum of antibodies from various patients to determine if they react with the sodium pump which contains ? and ? sub units. This will help in diagnosing pernicious anemia. A mouse is used in this experiment since its stomach structure is similar to that of a man. A primary antibody and a secondary antibody are also used in this experiment as they react with the mouse antigens. Anti proton pump of a human being can also cross react with proteins of the mouse making the mouse a favorable specimen for the experiment. From the experiment we expect antibody response to help us diagnose pernicious anemia. Aims The aim of this experiment is to determine whether samples from patients contain antibodies and also to diagnose patients having the disease through Western blotting; Immuno histochemistry using the gastric proton pump. Materials and Methods The materials used includes; SDS polyacrylamide gel (80%, resolving gel 40% upper stacking gel), Gel running buffer (25 mM Tris, pH 5.3, 0.192M Glycerin, 0.1% SDS), Gel apparatus, power supply, mouse stomach protein,5? SDS sample buffer (0.315 Tris, pH 6.8, 25% glycerol 10% SDS, 5% 2-beta mercaptoethanol, 0.025% Bromophenol blue), Protein MW standards, heat block, Gel loading tips, transfer apparatus, transfer buffer (25mM Tris, 192mM glycine,20% methanol), nitrocellulose membrane, filter paper, 0.1% Ponceau in1% acetic acid, 0.1M NaoH, TBS Tris buffered saline, Blocking solution TBS containing 5% skim milk powder. Materials required to obtain a mouse stomach include; a slide containing section of mouse stomach, xylene, hemoglobin, acid alcohol, Scott’s tap water, eosin, DPX mounting media and cover slips. Procedure to obtain mouse stomach morphology The slide containing mouse stomach is incubated in xylene and ethanol for two minutes respectively. After this the slide is rinsed in tap water for 30 seconds. The slide is then incubated in the hemoglobin for 2 minutes. The slide is rinsed again in tap water for 30 seconds after which it is placed in 1% acid alcohol for 3 seconds after which it is rinsed again in tap water for 30 seconds. The slide is incubated in Scott’s tap water for 30 seconds after which it is swashed in water for 30 seconds. The slide is then placed in eosin for 4minutes and excess is blotted off by a paper towel. The slide is then placed in 80% ethanol and again 90% after which the slide is incubated in ethanol for 2 minutes then allowed to air dry. A drop of DPX mounting medium is placed on the section and then covered by a cover slip. An observation is made under the microscope and diagrams made as follows. Fig 1 showing a mouse stomach with Haematoxylin To prepare the stomach protein sample, 200µl of protein is placed into a microfuge tube and 50µl 5? SDS sample buffer is added. The marker and the protein sample tubes are spine for a few seconds to bring the liquid to the bottom of the tube. The MW markers and proteins are then loaded using a special gel loading tips. The apparatus is then connected to a power supply of 200V and run for 1 hour until ...Download file to see next pagesRead More
Cite this document
  • APA
  • MLA
(“Molecular Basis of Disease Lab Report Example | Topics and Well Written Essays - 1500 words”, n.d.)
Retrieved from https://studentshare.org/biology/1451089-molecular-basis-of-disease
(Molecular Basis of Disease Lab Report Example | Topics and Well Written Essays - 1500 Words)
“Molecular Basis of Disease Lab Report Example | Topics and Well Written Essays - 1500 Words”, n.d. https://studentshare.org/biology/1451089-molecular-basis-of-disease.
  • Cited: 0 times
Comments (0)
Click to create a comment or rate a document
Molecular basis of aging in relation to fertility
With regards to the molecular aspects of aging, it is usually characterized by the accumulation of defective or inactivated forms of various enzymes, possibly due to oxidative damage by agents such as free radicals (Stadtman 131). A certain group of protein regulators that control enzymatic products called the polycomb group (PcG) control various gene loci that are essential to an organism, and one of these loci is the Ink4a locus, which contains genes responsible for senescence in organisms (Mishra and Mishra 135).
3 Pages(750 words)Essay
Staphylococcus and Streptococcus

The author states that microbes can be classified into various groups depending on their morphological and biochemical features. The Lancefield grouping is a method of clustering catalase-negative, coagulase-negative bacteria according to the carbohydrate structure of bacterial antigens present on their cell walls.

4 Pages(1000 words)Lab Report
Molecular Genetics Practical Write Up
Salmonella typhimurium is a leading cause of gastroenteritis in humans This experiment tested the histidine requirements of the auxotrophic mutant strains and their spontaneous reversion rate. The experiments were carried using either wild
7 Pages(1750 words)Lab Report
Molecular Basis of Disease
Cancer are abnormal human cells characterized by “unregulated growth, impaired cellular differentiation, invasiveness and metastatic potential” (Coleman, The Molecular Basis of Human Cancer, 2002, p.3). These characteristics of cancer cells have been used to clinically distinguish cancer cells from normal cells during diagnosis and treatment.
8 Pages(2000 words)Lab Report
Molecular Basis of Pernicious Anaemia
Others include infiltration of inflammatory cells into the stomach and production of auto- antibodies to various proteins. It also involves the intrinsic factor and proton pump of parietal cells whereby intrinsic factor can be identified as the factor that is involved in the absorption of vitamin B12.
6 Pages(1500 words)Lab Report
Molecular Fluorescence Analysis of Proteins
Fluorescence occurs when an excited molecule goes back to the ground state by photon emanation. Conversely, phosphorescence occurs when the excited electron
4 Pages(1000 words)Lab Report
The level of contamination on the things we use on daily basis
Name Institution Date of submission Abstract The main objective of the experiment was to ascertain the level of contamination on the things we use on daily basis to make our face that include brush, Chap Stick and mascaras. This was achieved by morphological test.
4 Pages(1000 words)Lab Report
Literature Critique Antibiotic Trials for Coronary Heart Disease
have been implicated in the pathogenesis of coronary artery disease (CAD) (Vijayvergiya, 2007). This interest has been stimulated by the frequent finding of bacterial antigen and occasionally recoverable organism within human atherosclerotic plaque and by seroepidemiologic studies.
6 Pages(1500 words)Lab Report
Molecular Biology
Abbreviations: Inr= initiator sequence of the RNA polymerase II promoter; UCE= upstream control element of the RNA polymerase I promoter. A 29 kilo base (kb) linear DNA fragment is digested with ApeK I, Bst I and Cla I, individually and in combination, and the resulting DNA fragment sizes are determined by agarose gel electrophoresis.
7 Pages(1750 words)Lab Report
Sexuality and Alzheimer's Disease
Sexuality continues throughout the life span, and even in old age sexual needs are quite common. During the first stage of Alzheimer’s disease in the elderly, either
3 Pages(750 words)Lab Report
sponsored ads
We use cookies to create the best experience for you. Keep on browsing if you are OK with that, or find out how to manage cookies.
Let us find you another Lab Report on topic Molecular Basis of Disease for FREE!
logo footer
Contact us:
Contact Us Now
FREE Mobile Apps:
  • StudentShare App Store
  • StudentShare Google play
  • About StudentShare
  • Testimonials
  • FAQ
  • Blog
  • Free Essays
  • New Essays
  • Essays
  • Miscellaneous
  • The Newest Essay Topics
  • Index samples by all dates
Join us:
Contact Us