quora
Nobody downloaded yet

Molecular Basis of Disease - Lab Report Example

Comments (0) Cite this document
Summary
Introduction Histology and molecular diagnostics play a critical role in the characterization of diseases in their most basic states and establishing the pathology of diseases. These techniques have been used in the characterization of various types of malignant cells in human beings and as a result act as necessary adjuvant in the study of oncology (Coleman, 2009, p.89)…
Download full paperFile format: .doc, available for editing
GRAB THE BEST PAPER92.4% of users find it useful
Molecular Basis of Disease
Read TextPreview

Extract of sample
"Molecular Basis of Disease"

Download file to see previous pages Staining is one of the techniques used in the characterization of cancer cells. The most commonly used staining methods and Hematoxylin and Eosin staining. Generally the two methods have been used to differentiate between the nucleus and the cytoplasm of cells. Hematoxylin stains the nucleus purple while Eosin stains the cytoplasms pink. Cancer cells can also be stained particularly to differentiate them from other cells. However, it is imperative to note that there are preparation processes that should take place in order for tissue staining to be specific and relevant (Ahmed, 2007, p.490). Histopathology is a technique that involves the studying of disease development in tissues at the microscopic level. In medicine histopathology refers to the examination of tissue biopsies that have already been prepared through histological techniques and placed on glass slides. The histological techniques used in sample preparation are tissue harvesting, fixation, embedding, mounting and staining of the tissue sections. Tissue harvesting: it involves the surgical removal of the tissue followed fixing to ensure that the tissue is stable and that it does not decay. Fixation: two methods are used; chemical fixation and freeze fixation. Chemical fixation is done using formalin while freeze fixation is done using cryo-protectants such as OCT, TBS or Cryogel before freezing. Mounting: Tissues are placed in paraffin before being sectioned using a microtome before they are stained. Staining: The processed tissues are stained for viewing under a microscope. Objectives I. To prepare low and high power drawings of normal skin, breast and colon tissues. II. To prepare low and high power drawings of one diseased skin specimen or one diseased colon specimen. III. To prepare low power diagrams of all three diseased breast tissue specimens IV. To answer the relevant questions in the practical manual Materials I. Light microscope. II. Blank plain paper. III. Pencil IV. Specimens: a) Colon - Normal human - Polyposis b) Skin - Normal human - Basal cell carcinoma - Malignant Melanoma - Squamous cell carcinoma c) Breast - Normal human - Lobular hyperplasia - Fibrodenoma Methods Human Colon The slide was held up to the light and the darker purple staining layer of the gastric mucosa (inner most layer of colon) identified. The slide was then place on the stage so that the inner layer of the colon was on top. The upper edge of the specimen was then focused under low power magnification of the microscope (X40) and the following areas identified: gastric mucosa, muscularis mucosa, sub-mucosa and muscularis. After the major layers had been identified, the following layers were labeled: mucosa, colonic glands, gastric pits, sub-mucosa, muscularis mucosa (inner and outer layers) and blood vessels. A high power drawing of the gastric mucosa was the prepared and the following labeled: surface epithelium, colonic glands, goblet cells and lymphoid follicles. The same procedure was repeated for diseases colon cells and the differences between tissue specimens identified. Human Skin The slide was held up to the light to identify the outer layer of the skin. The slide was then placed on the stage so that the surface of the skin was positioned on top. The upper edge of the specimen was focused under low ...Download file to see next pagesRead More
Cite this document
  • APA
  • MLA
  • CHICAGO
(“Molecular Basis of Disease Lab Report Example | Topics and Well Written Essays - 2000 words”, n.d.)
Retrieved from https://studentshare.org/biology/1446331-molecular-basis-of-disease
(Molecular Basis of Disease Lab Report Example | Topics and Well Written Essays - 2000 Words)
https://studentshare.org/biology/1446331-molecular-basis-of-disease.
“Molecular Basis of Disease Lab Report Example | Topics and Well Written Essays - 2000 Words”, n.d. https://studentshare.org/biology/1446331-molecular-basis-of-disease.
  • Cited: 0 times
Comments (0)
Click to create a comment or rate a document
CHECK THESE SAMPLES - THEY ALSO FIT YOUR TOPIC
Molecular basis of aging in relation to fertility
With regards to the molecular aspects of aging, it is usually characterized by the accumulation of defective or inactivated forms of various enzymes, possibly due to oxidative damage by agents such as free radicals (Stadtman 131). A certain group of protein regulators that control enzymatic products called the polycomb group (PcG) control various gene loci that are essential to an organism, and one of these loci is the Ink4a locus, which contains genes responsible for senescence in organisms (Mishra and Mishra 135).
3 Pages(750 words)Essay
Molecular Basis of Pernicious Anaemia
Others include infiltration of inflammatory cells into the stomach and production of auto- antibodies to various proteins. It also involves the intrinsic factor and proton pump of parietal cells whereby intrinsic factor can be identified as the factor that is involved in the absorption of vitamin B12.
6 Pages(1500 words)Lab Report
The level of contamination on the things we use on daily basis
Name Institution Date of submission Abstract The main objective of the experiment was to ascertain the level of contamination on the things we use on daily basis to make our face that include brush, Chap Stick and mascaras. This was achieved by morphological test.
4 Pages(1000 words)Lab Report
Molecular Basis of Disease
Vitamin B12 is normally absorbed in the walls of the ileum. The vitamin helps in maturation of red blood cells. According to Doctor Mellissa Conrad (Conrad, 1996), the large multinucleated cells known as megaloblasts circulate in the blood cells but do not act like blood cells.
6 Pages(1500 words)Lab Report
Literature Critique Antibiotic Trials for Coronary Heart Disease
have been implicated in the pathogenesis of coronary artery disease (CAD) (Vijayvergiya, 2007). This interest has been stimulated by the frequent finding of bacterial antigen and occasionally recoverable organism within human atherosclerotic plaque and by seroepidemiologic studies.
6 Pages(1500 words)Lab Report
Molecular Biology
Abbreviations: Inr= initiator sequence of the RNA polymerase II promoter; UCE= upstream control element of the RNA polymerase I promoter. A 29 kilo base (kb) linear DNA fragment is digested with ApeK I, Bst I and Cla I, individually and in combination, and the resulting DNA fragment sizes are determined by agarose gel electrophoresis.
7 Pages(1750 words)Lab Report
Sexuality and Alzheimer's Disease
Sexuality continues throughout the life span, and even in old age sexual needs are quite common. During the first stage of Alzheimer’s disease in the elderly, either
3 Pages(750 words)Lab Report
Staphylococcus and Streptococcus

The author states that microbes can be classified into various groups depending on their morphological and biochemical features. The Lancefield grouping is a method of clustering catalase-negative, coagulase-negative bacteria according to the carbohydrate structure of bacterial antigens present on their cell walls.

4 Pages(1000 words)Lab Report
Molecular Genetics Practical Write Up
Salmonella typhimurium is a leading cause of gastroenteritis in humans This experiment tested the histidine requirements of the auxotrophic mutant strains and their spontaneous reversion rate. The experiments were carried using either wild
7 Pages(1750 words)Lab Report
Molecular Fluorescence Analysis of Proteins
Fluorescence occurs when an excited molecule goes back to the ground state by photon emanation. Conversely, phosphorescence occurs when the excited electron
4 Pages(1000 words)Lab Report
sponsored ads
We use cookies to create the best experience for you. Keep on browsing if you are OK with that, or find out how to manage cookies.
Let us find you another Lab Report on topic Molecular Basis of Disease for FREE!
logo footer
Contact us:
+16312120006
Contact Us Now
FREE Mobile Apps:
  • StudentShare App Store
  • StudentShare Google play
  • About StudentShare
  • Testimonials
  • FAQ
  • Blog
  • Free Essays
  • New Essays
  • Essays
  • Miscellaneous
  • The Newest Essay Topics
  • Index samples by all dates
Join us:
Contact Us