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Action Of Enzyme Catalase On Hydrogen Peroxide - Lab Report Example

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Catalase is an enzyme present in the cells of plants, animals and aerobic (oxygen requiring) bacteria. It promotes the conversion of hydrogen peroxide, a powerful and potentially harmful oxidizing agent, to water and molecular oxygen…
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Action Of Enzyme Catalase On Hydrogen Peroxide
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"Action Of Enzyme Catalase On Hydrogen Peroxide"

Download file to see previous pages The student feels that the more the concentration of the liver the more the enzyme that would result since more amount of oxygen would react with the hydrogen peroxide molecules. For accomplishing this, the student has taken five test tubes with the first test tube having twice the suspension as that of the fifth tube expecting that there would be twice the amount of oxygen given off. This seems that the student wants to prove that the amount of oxygen that would be evolved would be directly proportional to the amount of the liver suspension take. Since it cannot be decided in a single experiment the student repeats it three times to observe whether the desired changes have taken place in each experiment or not. The independent variable to be considered here would be the amount of liver suspension and distilled water and the dependent variable would be the amount of oxygen evolved. This experiment deals with quantitative analysis.
The overall experiment can be summed up by the following table

(Note: Exp indicates experiment)

It can be observed that there is a significant variation in the amount of oxygen evolved in the three experiments. The variation can be clearly understood observing the graph below
It can be inferred from the graph that there is a gradual decline in the amount of oxygen evolved considering the concentrations of the enzyme. (The x-axis represents experiment and the y-axis represents the amount of oxygen evolved). It is only in the second experiment that the discrepancy arises. It is highly probable that there might have been a replacement of the suspension. It can be clearly understood that there is a direct relationship between the amount of suspension and the amount of oxygen evolved.
The experiment is in fact a relatively simple one and can be performed easily if the conditions are ideal. The student has done it appropriately and has a good analytic skill. The prediction is purely based on understanding as it involves mainly quantitative analysis rather than the chemical reaction.
The student has opted the right materials for accomplishing this experiment. The only requirement would be to do the experiment under perfect conditions and conduct the experiment singly so as not to confuse with other experiments.
The following experiment would boost the above observation
Iftime is increased, then more hydrogen peroxide molecules will be split into water and ...Download file to see next pagesRead More
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An Enzyme Controlled Reaction-Catalase

The author provides an experiment, which has 1 independent variable, 1 dependent variable and 1 control variable. The independent variable is the various concentrations of hydrogen peroxide, while the dependent variable is the rate of reaction in terms of oxygen production. The control variable is the temperature of the room and standard pressure.

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