Biomedical Science - Lab Report Example

A Tetanus Toxoid T lymphocyte Proliferation Assay College: Tetanus Toxoid (TT) µg/ml count (cpm) count (cpm) count (cpm) Average count (cpm)count (cpm) in logNo antigen22171686184919173.28TT 0.01105041086711117108294TT 0.1322652913537944331154.5TT 19454110216996627977795TT 101267901232371181401227225.1TT 1001257851284341213271251825.13.1Ovalbumin 1678126711281358(100 mg/ml)Table 1.Count of PBMC’s in logs and the toxoids conc in Ug/mlFigure 1.Graphical presentation of the data cpm against the Tetanus toxoids (log scale)a).

Which concentration of TT gave maximum T cell proliferation?10 µg/mlb).Which APC are present in the PBMC?Dendritic CellsB cellsMonocytesMacrophages c) Why was ovalbumin used in this experiment?Since it is a T-cell dependent antigen it was used as a protein model to study antigen specific immune response in the mice. It was also used because it is inert and non-specific hence doesn’t cause harm to the mouse even after the immune response is not induced.d).Discuss the results obtained.Basing on the graph above, it is clearly evident that the toxoids induced the immune response.

When the concentration of the toxoids was increased so does the PBMC’s.The maximum amount of the toxoids that is able to induce the immune response is 100ug/ml and at this point the maximum immune cells that are generated from this are about 122722cpm. The proliferation of Antigen-specific T-cell normally employed as an assay for the responses of T-cell (Zhang et al,1998). Humans or mice T cells immunised with a given antigen. For instance, in this case tetanus toxoids, they have a capacity of proliferating after being exposed to the antigen presenting cells as well as tetanus toxoids but this can’t be from the antigens related to it that had not been immunized.

The measurement of proliferation can be done by incorporating of 3H-thymidine into the actively dividing cells DNA (Ehl et al, 1997). The proliferation of antigen-specific is a specific CD4 T cell immunity hallmark (Tough et al, 1996).BibliographyTough, D. F., Borrow, P. and Sprent, J.(1996) Induction of bystander T cell proliferation by viruses and type I interferon in vivo.Science.272:1947–1950.2Zhang, X., Sun, S., Hwang, I., Tough, D. F. and Sprent, J. (1998).Potent and selective stimulation of memory-phenotype CD81T cells in vivo by IL-15.Immunity.8: 591–599.3Ehl, S., Hombach, J.

, Aichele, P., Hengartner, H. and Zinkernagel, R. M.,Bystander (1997)activation of cytotoxic T cells: studies on the mechanism and evaluation ofin vivo significance in a transgenic mouse model.J. Exp. Med..185: 1241–1251