We use cookies to create the best experience for you. Keep on browsing if you are OK with that, or find out how to manage cookies.

Gene technology - Lab Report Example

Comments (0)
Summary
The size of genomic DNA from E.Coli is about 4,500 kb which is beyond the limit of the gel or the DNA ladder used. Appearance of E.Coli DNA is shown in Figure 1b.
Interpretation…
Download full paper
GRAB THE BEST PAPER

Extract of sample
Gene technology

Download file to see previous pages... The plasmid is 3.5 kb in size (Fig.5). It has two ECoRI restriction sites, hence should break any recombinant DNA into two fragments, i.e. ~3.5 kb and the inserted DNA.
In our experiment – There are three fragments of DNA which shows that the inserted PCR product must have one ECoRI site. The ECoRI site in the PCR product is placed almost in the middle breaking it into two fragments, viz. 600 bp and 800 bp (Fig. 6).
Interpretation - It appears that the insert has two restriction sites for NCoI (Fig 7). But position of the sites would result in a DNA fragment smaller than 1.5 kb (the total size of the insert), the size discrepancy is difficult to explain.
Interpretation – This result is totally unexpected! The bands corresponding to both the restriction enzymes, i.e. at 1.0 kb, 2.5 kb, 3.0 kb are there but in addition there are three other intermediate size DNA fragments. The bands appear to be intermediates produced due to impartial digestion of the recombinant by the two restriction enzymes.
Reason – Concentration of the enzymes was less, time of incubation is less than optimum or incubation conditions were inadequate. Sufficient time should be given for the restriction enzymes to act on all the sites.
ii. NcoI digestion (Lane 4) – the restriction sites appear to be different than mine (Fig 4a). The NcoI sites appear to be closer in the 16S rDNA extracted by this student and give rise to a DNA fragment of 600 bp.
Janda M. and Abbott S.L. (2007). 16S rRNA Gene Sequencing for Bacterial Identification in the Diagnostic Laboratory: Pluses, Perils, and Pitfalls. J. Clin Microbiol. 2007 September; 45(9): 2761–2764.
Khare N., Sharma D., Somashekar U., Prakash A., Prakash S., Mendki M.J. and Anvikar A. (2008). Detection of bacterial DNA in cholesterol gall stones. The Internet Journal of Surgery 16 (2). Available from ...Download file to see next pagesRead More
Comments (0)
Click to create a comment
CHECK THESE SAMPLES - THEY ALSO FIT YOUR TOPIC
Gene Splicing lab
5 Pages(1250 words)Lab Report
Gene Prediction
It involves pasting the whole genome sequence of the organism given in a FASTA format then clicking the ORF finder that will automatically give the start and the stop codons alongside the longest pattern. In this case, the human genome was given and upon subjecting it to
2 Pages(500 words)Lab Report
Student practical report (3 activities in one report). Activity 1: Semi-quantitative gene expression by reverse transcriptase- PCR (RT-PCR) analysis. Activity 2: Extract total RNA from mammal tissues. Activity 3: gels result
ast tumour and normal tissue specimens, and compare the amplified target cDNA to a constitutively expressed house-keeping gene, glyceraldehyde-3-phosphate dehydrogenase (GAPDH). Dysregulated cell cycle control is a primary mechanism of cancer (Deshpande, Sicinski & Hinds,
6 Pages(1500 words)Lab Report
DNA Barcoding Invertebrate Lab Report #1
The first stage is to obtain a specimen which may be from from tissue or culture collections. In the second stage, laboratory analysis which involves extraction of genetic material to obtain DNA batcode sequence is done. These sequences are later
2 Pages(500 words)Lab Report
Experiment to determine the presence of a single nucleotide polymorphism in a gene
The SNP genomic distribution has no homogeneity. It always occurs in those regions that are non-coding. It has minimum occurrence in the regions that are coded. This is a case where the natural selection acts and fixes the SNP allele with favorable adaptation
4 Pages(1000 words)Lab Report
Detecting Cry1Ab gene in Mon0810 Transgenic Maize by PCR
After DNA is extracted from the maize, gel electrophoresis is done using agarose gel for the visibility of the DNA strands. A photograph of the amplified genes is then taken using a UV Trans illuminator. The photograph will provide a hard or soft copy of the DNA strands that
1 Pages(250 words)Lab Report
Bacterial transformation and gene expression
This research aims to evaluate and present bacterial transformation and gene expression. The research will demonstrate two important genetic engineering tools used for the expression of the foreign gene of interest in the given bacterial cell and three conditions required for the transformation.
8 Pages(2000 words)Lab Report
Per3 Gene and Diurnal Preference

The studies have found that a biallelic VNTR (variable number tandem repeat) polymorphism is present in the human PER3 gene at the chromosome 1p36.23, and it contains two alleles with 4 or 5 tandem repeats of 54 bp.This VNTR was evaluated as the important genetic factor for the chronotypes as it is associated with the diurnal preference.

7 Pages(1750 words)Lab Report
Manufacturing Technology lab
The stock billet was measured for its diameter and length, after which it was affixed in the lathe machine’s chuck, making sure that the piece was centred well. The chuck was spun by hand and measurements were taken off
6 Pages(1500 words)Lab Report
Design and Technology
Design for assembly can be defined as the process of systematic analyses aimed at simplifying the structure of the product as well as reducing the products assembly cost. The assembly quality of the product is associated with the number of parts. The DFA technique can enable the rating of the assimilability by the designers quantitatively.
4 Pages(1000 words)Lab Report
Let us find you another Lab Report on topic Gene technology for FREE!
Contact us:
+16312120006
Contact Us Now
FREE Mobile Apps:
  • About StudentShare
  • Testimonials
  • FAQ
  • Blog
  • Free Essays
  • New Essays
  • Essays
  • The Newest Essay Topics
  • Index samples by all dates
Join us:
Contact Us