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Biotechnology - Essay Example

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However, the production involves multiple processes that range from the upstream processing and to the downstream processing that ensures that the…
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Download file to see previous pages However, a more specific approach of the upstream processes will be adopted in this essay. Downstream processes refer to the processes that take place after the fermentation process of purification to the packaging of the Biopharmaceuticals. Moreover, the issue of quality control techniques in a bid to ensure successful product analysis will receive attention in this essay.
One of the critical steps in the upstream processing is the choice of an effective culture and expression system.There are three types of culture systems that may be adopted in the fermentation process. One of them is the batch culture, which is the commonest culture system that used in many industries because of lack of complications. The fact that batch bioreactors are easily available explains why many companies have exhibited preference for it. Batch cultures need to be loaded with all the required nutrients and substrates, and then inoculated with the selected microorganism. Although it is commonly referred to a closed system, there is an evident need to maintain a measure of exerting control on factors such as pH and aeration. After fermentation runs to completion, product removal follows (Boudreau & McMillan, 2007).
Although the batch system has been highly preferred, it presents certain challenges, especially because of the surging lack of constancy in the producing formation. In the initial phase when the microorganism is undergoing growth, there is no productivity. Moreover, accumulation of the product also limits further production (Buckel, 2001). In other cases, the presence of a high substrate concentration in the initial phase serves as a form of inhibition. In a bid to address this challenge, the development if fed batch where an inflow is introduced was a benchmark of success. Although the batch culture still has some of the outstanding disadvantages of the batch culture, the fed-batch ...Download file to see next pagesRead More
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