The peptic digest was subjected to chromatography on the weak cation-exchange resin, Macro-prep CM. Two protein fractions F1 and F2 corresponding to two eluate peaks were collected using 10% acetic acid as…
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Results indicated antibacterial activity to be present in pepsin-digested lactoferrin and F2 sample. Thus, purification of lactoferricin obtained through peptic digestion of bovine lactoferrin was accomplished by fractionation of the hydrolysate using cation exchange chromatography.
Lactoferrin (Lf) is an iron-binding glycoprotein and, as the name suggests, is a constituent of milk. It is also found, to a lesser extent, in various mucosal (exocrine) secretions of mammals that are commonly exposed to normal flora such as tears, nasal exudate, saliva, bronchial mucus, gastrointestinal fluids, cervicovaginal mucus and seminal fluid (Weinberg, 2003). Also, Lf is produced by secondary granules of polymorphonuclear neutrophils for deposition at septic sites. The granular contents of neutrophils can be released into inflammatory fluids after neutrophil death, by what is known as "holocrine secretion". The protein is closely related to transferrin, the iron-transport protein present in the plasma. Lactoferrin is a multifunctional innate-defense protein, known to exert a broad-spectrum primary defense activity against microbes including bacteria, fungi, protozoa and viruses (Orsi, 2004), and even some antibiotic-resistant pathogens (Wakabayashi et al., 2003). Lf is found in high concentrations in breast milk (~3–7 mg/ml) and tear fluid (1–4 mg/ml) (Rogan et al., 2006). Among the many constituents of milk which have revealed antimicrobial activity, lactoferrin exhibits both bacteriostatic and bactericidal activity against a wide array of microorganisms, including those causing gastroenteric infections, food poisoning, listeriosis and mastitis (Dionysius et al., 1993). Recently, clinical trials have demonstrated that bovine lactoferrin (bLF) administration can reduce the risk of colon carcinogenesis in humans (Tsuda et al., 2010). A key role of Lf is to scavenge non-protein-bound iron in body
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Proteins vary in their structure and also functions. The author describes specific functions of proteins. Proteins that are specialized in defending the body from antigens are known as antibodies. Those responsible for movement are known as contractile protein and examples of these are actin and myosin.
Ub is found throughout an entire cell and can exist in a free state or combined with other protein. If it is combined with other protein then it is attached by a covalent bond between the glycine and lysine on the proteins. Ub has a role to play in many cell processes. For example – during the G1 phase of mitosis Ub is conjugated to the protein cyclin and hence play an important role in controlling the cell cycle. Ub conjugation is also present in DNA repair, the regulation of transcription, apoptosis and embryogenesis.
According to the paper it is very important for every individual to take care of his nutrition. It is of crucial importance to be aware of the important role vitamins, micro- and macro elements play in our nutrition and contribute much to our lives. Further discussion deals with proteins. These are polymer chains, which consist of amino acids connected by peptide bonds.
Amino acids are involved in the formation of DNA and RNA which forms the backbone of the human genome (genes). Dietary source of proteins includes eggs, milk, meat, soya and beans (Millward, 2007). This paper will focus on
These include that the protein contents must be transported to the assigned submitochondrial section, and that the protein contents to be routed to the innermost compartments (the matrix) have to be routed across two other membranes (Kanner et
Renal functions hence show significant changes with aging. Due to aging, rats develop renal pathology such as; tubular casts, glomerulosclerosis (Kachirskaia, 2008), interstitial fibrosis and infiltration by inflammatory cells this in turn explain the impaired
This paper concerns the observing and studying with the revolutionary prospectus in pharmaceutical perspectives, snake venom could pay the way for the fresh age of medicines, as well as the research for treatment of cancer among other illnesses. Snake venoms are often studied by scientists and researchers for its therapeutically/remedial application.
Study outcomes confirms that students who engage in more physical exercises have lower level of stress compared to those who engage in less physical activities. Stress is highly reduced when students exercise together
From the muscles, the alanine is absorbed in the liver where the process of transamination occurs. The process occurs in the presence of alpha-ketoglutarate. This cycle generates pyruvate which enters the gluconeogenesis cycle and glutamate which enters the
The samples will include 40 ninth-grade students from a school in Saudi Arabias capital, Riyadh. The model will be equally divided into terms of gender distribution. The control group will be equally extracted from the 20 males and 20 females. The
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