Nobody downloaded yet

Investigate osmosis in blood cells - Lab Report Example

Comments (0) Cite this document
Introduction: The erythrocyte membrane accounts for 1 percent of total weight of the red cell, yet it plays an integral role in the maintenance of erythrocyte integrity. The red cell membrane and its skeleton provide the erythrocyte the flexibility, durability, and tensile strength to undergo large deformations during repeated passages through narrow microcirculatory channels…
Download full paperFile format: .doc, available for editing
GRAB THE BEST PAPER94.3% of users find it useful
Investigate osmosis in blood cells
Read TextPreview

Extract of sample "Investigate osmosis in blood cells"

Download file to see previous pages The cellular geometry, that is, the biconcave disc shape of red cells, is critical for the cells' survival. This cell surface shape provides a high ratio of surface area to cellular volume. The normal volume of the erythrocyte is approximately 90 m3. The minimum surface area that could encase this volume is a sphere of approximately 98 m3. The surface area of a biconcave disc enclosing this volume is approximately 140 m3. Thus, shape alone provides the red cell with a considerable amount of redundant membrane and cytoskeleton. This feature provides the extra membrane surface area needed when red cells swell. More importantly, this geometric arrangement allows red cells to stretch as they undergo deformation and distortion in response to the mechanical stress of the circulation. The consequent reduction in tolerance of these cells to osmotic stress explains why anaemias resulting from membrane defects often are accompanied by osmotic fragility, the basis for the clinical laboratory test. Similarly, if erythrocytes are engorged with water, they become macrospherocytic and less deformable (Dacie, J. V., Lewis, S. M., and Luzzatto, L., 1981).
Red Cell Membrane Permeability: The normal red cell membrane is nearly impermeable to monovalent and divalent cations, thereby maintaining a high potassium, low sodium, and very low calcium content. In contrast, the red cell is highly permeable to water and anions, which are readily exchanged. As a result, erythrocytes behave as nearly perfect osmometers. Water and ion transport pathways in the red cell membrane include energy-driven membrane pumps, gradient-driven systems, and various channels. An important feature of the normal red cell is its ability to maintain a constant volume. The mechanisms by which red cells "sense" changes in cell volume and activate appropriate volume regulatory pathways are unknown. The effects of disruption of the red cell permeability barrier are illustrated by complement-mediated hemolysis. Complement activation on the red cell surface leads to formation of the membrane attack complex, which is composed of terminal complement components embedded in the lipid bilayer. This multimolecular complex acts as a cation channel, allowing passive movements of sodium, potassium, and calcium across the membrane according to their concentration gradients. Attracted by fixed anions, such as hemoglobin, ATP, and 2,3-BPG, sodium accumulates in the cell in excess of potassium loss and of the compensatory efforts of the Na+-K+ pump. The resulting increase in intracellular monovalent cations and water is followed by cell swelling and ultimately colloid osmotic hemolysis (Dacie, J. V., Lewis, S. M., and Luzzatto, L., 1981).
Rationale of the Test: Osmotic activity in the red cells is tested by adding increasingly hypotonic concentrations of saline solution to red cells. As a result of osmosis, more and more water from the increasingly hypotonic solution will enter the red cells leading to increased volume of red cells by swelling. If the concentration goes beyond threshold, more water will enter into the cells which already are at maximum volume for surface area, and will burst at the most hypotonic normal saline concentrations. However, after incubation at 37C (98.6F) for 30 mins, these red cells will lose membrane surface area more readily than normal because their ...Download file to see next pagesRead More
Cite this document
  • APA
  • MLA
(“Investigate osmosis in blood cells Lab Report Example | Topics and Well Written Essays - 1250 words”, n.d.)
Investigate osmosis in blood cells Lab Report Example | Topics and Well Written Essays - 1250 words. Retrieved from
(Investigate Osmosis in Blood Cells Lab Report Example | Topics and Well Written Essays - 1250 Words)
Investigate Osmosis in Blood Cells Lab Report Example | Topics and Well Written Essays - 1250 Words.
“Investigate Osmosis in Blood Cells Lab Report Example | Topics and Well Written Essays - 1250 Words”, n.d.
  • Cited: 0 times
Comments (0)
Click to create a comment or rate a document

CHECK THESE SAMPLES OF Investigate osmosis in blood cells

The Red Blood cells

...?Section A Calculation for RBC Length of one smallest square 20 mm Width of one smallest square 20 mm Depth of one smallest square 10 mm Volume of one smallest square = 1/20 x 1/20 x 1/10 = 1/4000 mm3 Total number of RBCs counted = 1036 (5 squares, 4 corners + 1 center) No. of RBCs in one smallest square = 1036/5 = 207.2 No. of RBCs in 1mm3 undiluted = 207.2 x 10000 = 2072000 / mm3 The Red Blood cell count according to the improved hemocytometer is 2.07 x 106 mm3. According to this reading the person seems to be anemic which may be due to iron deficiency, folate deficiency, genetic disorders or immune dysfunction. The difference between two readings could be due to many reasons. The high value in the second test can be attributed... to...
6 Pages(1500 words)Lab Report

White Blood Cells (WBC)

...? White Blood Cells (WBC) White Blood Cells (White Blood Cells: Battling Blood Cells, Our Blood consists of Red and White Cells which have important role in maintaining our body healthy and keeping bodily metabolisms intact. White Blood Cells (also called leucocytes) are responsible for keeping our immune system working all the time to prevent the intrusions of foreign materials or infectious diseases into our body. Bone marrow is the producer of White Blood Cells. The major function of Hematopoietic stem cells, found in bone marrow, is the production of WBC. Hematopoietic stem cells have a life span of only three to four days and therefore, any deficiencies in the production of this stem cell may affect the production of WBC also... . “The...
3 Pages(750 words)Research Paper

Lab Report Of An Experiment To Find Out the Example of Passive Transport Processes in Cells absorbed and passed into the blood stream while carbon dioxide is expelled from the blood stream to the outside through the nose. Lastly, in the kidney, excess salt is absorbed and later expelled out of the body as urine. Conclusion Through the process of osmosis, water was able to move from the beaker where it was in high concentration into the dialysis tube where its concentration was low. On the other hand, there the process of diffusion worked to realize the movement of glucose from the dialysis bag where the concentration was high into the beaker where its concentration was low. Therefore, the experiment successfully showed the process of passive transport across the...
5 Pages(1250 words)Essay


...Osmosis Experiment with Swede In this experiment, I tried to find out the effect of osmosis on slices of swede, a root vegetable, immersed in a pan of water solution. Osmosis is a process where water passes in or out through the tissue of the plant or vegetable. This process explains how plants or vegetables absorb water into its cells or release water from its cells. How does osmosis take place A plant or vegetable is full of cells that contain water. Each of these tiny cells is surrounded by a membrane like our skin. The membrane protects the cell by keeping its parts inside and...
4 Pages(1000 words)Essay

Diffusion and osmosis

.... The first part demonstrated the effect of temperature on the rate of diffusion. Crystals of potassium permanganate were placed in beakers which were subjected to different temperatures. Changes in the solutions were observed over a period of time. Observations were noted and recorded at 0 minute, 5 minutes and then at 10-minute intervals for the first hour, and then again after every 20 minutes thereafter. To demonstrate the effect of osmosis on red blood cells, six test tubes were prepared and were labeled test tubes A-F. Two (2) ml of different solutions were placed in each tube with the use of a Pasteur pipette. Two (2) ml of blood was then added to each test tube. After mixing by inversion, the tube was placed in front... Running...
6 Pages(1500 words)Essay

Osmosis paper

...Section: submitted: OSMOSIS Osmosis is the movement of water molecules from an area of higher concentration to an area of lower concentration through a selectively permeable membrane (Miller and Levine, 186-187). But, the basic question is, how would this rate of movement or diffusion of water molecules through a selectively permeable membrane be affected by the differences in concentrations of sucrose? Most likely, if the concentration of sucrose in a solution is low, then the rate of diffusion of water molecules through a selectively permeable membrane would also be low. On the other hand, if the concentration of sucrose in a solution is high, then the rate of diffusion of water molecules through a...
2 Pages(500 words)Essay

Egg Osmosis

...thermodynamic activity, water movement, or osmosis, should go from a low (hypotonic solution) to high concentration (hypertonic solution) of solutes. If no osmosis was observed, the cytoplasm is said to be isotonic to the surrounding solution. Osmosis happening in cells was replicated in this experiment, with unshelled eggs representing the cells with differentially permeable plasma membrane. The objective of this experiment was to observe the effects of changing the extracellular concentration s to the amount of water within the cell. Methods Six unshelled eggs of predetermined weight were subsequently submerged in 0%, 10%, 20%,...
6 Pages(1500 words)Lab Report

Red Blood Cells Cytoskeleton

...Your full November 18, Red Blood Cells Cytoskeleton Red blood cells have a specific biconcavedisc shape. This characteristic enables them to optimize their ability of gas exchange. They have a 120 day life span, and throughout this course, the red blood cells experience high intensity sheer force while moving through narrow capillaries in the microvasculature. This results in fast but reversible deformations in their structure. Thus, there is special cytoskeleton that the red blood cells are equipped with, which helps them to combat this sheer force by providing them enough mechanical firmness and...
1 Pages(250 words)Article


... Cells Our body consists of small particles, which are called cells. Every cell is a small alive organism: it can feed, reproduce and interact with other cells. Many cells of one type form tissues our organism consists of. Every cell consists of nucleus surrounded by cytoplasm and covered with thin covering - membrane. Cytoplasm is water, which contains different nutritious substances such as carbohydrates, proteins etc. nucleus contains the substance called DNA, which contains the coding of all the genetic information. Different types of cells fulfill different tasks, but all of them are equally structured. Cellular membrane separates the content of cell from the outer environment and fulfills the metabolism between the cell... and the...
3 Pages(750 words)Admission/Application Essay

Potato Osmosis

...Diffusion & Osmosis Introduction All living organisms including plants and animals have evolved from the simplest unicellular organisms like the planktons and amoebae to the much more complex living forms with intricate cellular differentiation and organization. But even the simplest unicellular protozoan needs to communicate with the external environment for survival because it needs to ingest food, eliminate waste products and toxins of metabolism and exchange gases through respiratory mechanisms. This communication is further developed in a more intricate manner in the multicellular organisms where exchanges take place within and between the cells in carrying out their assigned physiological functions....
9 Pages(2250 words)Assignment
sponsored ads
We use cookies to create the best experience for you. Keep on browsing if you are OK with that, or find out how to manage cookies.

Let us find you another Lab Report on topic Investigate osmosis in blood cells for FREE!

Contact Us