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Animal Oxygen Consumption - Lab Report Example

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Summary
This laboratory activity is concerned about energy metabolism absorbed in the energy production among animals which were used as subjects of the study. Primarily, it demonstrated relationship of the subjects' body weight, oxygen consumption per body weight at certain incubation periods…
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Animal Oxygen Consumption
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Download file to see previous pages After series of tests and manipulation, the investigator found out that, oxygen consumption of the group's subject which is the "Tilapia" increases, as incubation period increases; however, the former was found to have no direct relationship with its body weight. The result was contrasting to the experience of other groups which were assigned to investigate "crayfishes". Direct or linear relationship among crayfishes' oxygen consumption, incubation time and body weight were not as well established.
All living cells need energy from exterior sources to act upon their many important tasks such as biosynthesis, transportation of molecules across membranes, movement, and reproduction. Green plants for example, acquire vast amount of energy from sunlight through photosynthesis. Chloroplasts surrounding the cell matrix convert solar energy into chemical energy. Moreover, most animals ingest food (usually plants and other animals) to acquire chemical energy that are stored in the food at the same time generate ATP through a process called cellular respiration. Cellular respiration in the same manner requires oxygen as a reactant. Thus, as an outcome of cellular respiration and cellular activity, animals are able to produce and release heat. This is one of the many unique characteristics of organisms and they as well vary in the rate of heat production as part of their metabolism.
The overall process of cellular respiration can be summarized as:

C6H12O6 + 6 O2 6 CO2 + 6 H2O + ATP + heat
(Foodstuff) (respiration) (heat) (work)
Oxygen consumption was measured by estimating the energy metabolism, since the rate of oxygen consumption as well as energy utilization is generally directly related.
In this exercise, the oxygen consumption of Tilapia was determined by using LaMotte Dissolved Oxygen kit in small volumes of water. This technique is titration-based on the oxidizing characteristics of dissolved oxygen (DO). Manganese solution is also added to the tested water samples followed by a strong alkali. Later, the solution was titrated through a standard solution, followed by addition of an indicator.
Objectives
The laboratory activity intended to achieve the following aims:
1. To demonstrate relationship in animals' body weight, oxygen consumption per body weight with respect to the incubation periods.
2. To perform accurately the procedures in the use of LaMotte Dissolved Oxygen kit, titrations and balance.
3. To compare the oxygen consumption of nektonic and benthic organisms.
Hypothesis
There is no direct relationship among oxygen consumption, incubation time and body weight for both animals (tilapia and crayfish).
Methods/Procedures
1. Students are assigned into groups. Each group will be assigned and organism to work with depending on availability.
2. Fill 4 jars with the aquarium water. Using a net, select 3 animals of which your group is assigned to and gently transfer them one to each of the mason jars. The animals may vary in size. In addition, in the case of Tilapia, choose smaller ones so they have room to move about in the jar. *Collect water for your negative control first, since ...Download file to see next pagesRead More
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