Interpretation of Immunological Tests - Essay Example

This is because, in this fraction, serum was not diluted. Therefore, aggregation of proteins could not occur because of the lack of hydration of protein. The protein concentration of F1 was 5.90mg. This increased to 14.56mg protein concentration for F3. Fraction F5, which is a mixture of F2 and F3 had a protein concentration of 16.00mg – the highest.

Purification by ammonium sulfate precipitation is useful for large protein samples. It is a cheaper process. However, the resulting protein obtained is not very pure. Moreover, this technique requires additional purification steps.

Section 3:The results of purification through ion exchange chromatography (IEX) are shown in section 3 of the results. The purification of IgG was performed. IgG accounts for only 10% of serum proteins. Yet, the concentrations of IgG in fractions F2, F3, and F5 were 2.08, 1.42, and 2.

80 mg/ml respectively, which are relatively high. IEX is a cost-effective process and results in higher protein recovery. It has high resolving power. Section 4:The percentage yield of protein for all fractions is given in section 4. A higher yield of IgG is seen in fractions F2 and F5 compared to fraction F3. A machine error may be the reason behind this. This is because the reading for F2/F3 and F5 was taken on different spectrophotometers.

Section 5:The results of the Single Radial Immunodiffusion assay and precipitin arcs are shown in section 5.
In figure 1, purple precipitin arcs confirm the presence of IgG. Multiple arcs for F3 and F4 indicate the presence of contamination in these protein fractions. In figure 2, the precipitation ring is observed. It is seen that as the diameter of the ring increases, there is a decrease in the protein (antigen) concentration. Moving further away from the center, IgG concentration decreases.