Contact Us
Sign In / Sign Up for FREE
Go to advanced search...

Bacterial cells Quantification - Lab Report Example

Comments (0) Cite this document
The Effect of Lysozyme Solution on Micrococcus lysodeikticus Cell Counts Introduction Bacterial cell quantification is useful in many areas of biology, in that it allows us to know the number of cells, or the viable cell count. This information can be used in antibiotic sensitivity testing, or infection severity, for example…
Download full paperFile format: .doc, available for editing
GRAB THE BEST PAPER91.7% of users find it useful
Bacterial cells Quantification
Read TextPreview

Extract of sample "Bacterial cells Quantification"

Download file to see previous pages Additionally, the information provided by this can be used to discover the effect of an enzyme on bacterial cell count – if an enzyme increases reproduction or lyses cells, this is discoverable by comparison to the absorbance graph created using known concentrations. In this experiment, the activity of lysozyme is studied. Lysozyme is the name of a glycoside hydrolase which damages cell walls by catalysing hydrolysis of the links between two key components of peptidoglycan – N-acetylmuramic acid and N-acetyl-D-glucosamine (Pommerville, 2007). It is notable that lysozyme is present in many human excretions, such as saliva and tears, and this forms one of the body’s many natural antimicrobial techniques. It is also notable that lysozyme is more effective against Gram-positive cells because the cell walls of these bacteria have a higher concentration of peptidoglycan (Pommerville, 2007). An additional experiment was performed to test the effect of certain antibiotics on bacterial cells. This is always an important test because of the current crisis in clinical practice due to the increasing amount of bacteria that are highly resistant to many or all of the available antibiotics (Neu, 1992). This can be done in several ways, but antibiotic disc sensitivity testing is particularly useful as it allows a test of several antibiotics on one sample of organism, allowing us to be certain that all the microbes are the same and thus reducing the possibility of false results. Results Antibiotic Effect on Various Bacteria Antibiotic/Dose Escherichia coli (-) Pseudomonas aeruginosa (-) Staphylococcus aureus (+) Bacillus subtilis (+) AP/25?g S R S S GM/10?g S S R S PY/100?g S R S S NA/30?g S R S R NI/50?g R R S S SM/200?g R R R R T/100?g S R S R TS/25?g S R S S NI at a dose of 50?g is effective only against Gram-positive microbes. TS at a dose of 25?g and T at a dose of 100?g, as well as NA at 30?g, PY at 100?g, GM at 10?g and AP at 25?g all appear to be effective against both Gram-positive and Gram-negative organisms. Micrococcus lysodeikticus Concentration vs. Absorbance at 450nm Micrococcus lysodeikticus (cells/ml) Absorbance at 450nm 0 0.000 6.25 x 106 0.406 1.25 x 107 0.274 2.5 x 107 0.301 5.0 x 107 0.455 1.0 x 108 0.870 Unknown 0.599 The general trend appears to be that absorbance is higher with a higher concentration of cells per ml. Absorbance = (0.000000008 * cell concentration) + 0.21 We can thus work out the unknown value by rearranging this formula – Absorbance – 0.21 = 0.000000008 * cell concentration Cell concentration = (Absorbance – 0.21)/0.000000008 Unknown = 48625000 or 4.8625 x 107 Effects of Lysozyme Solution on Viable Cell Count – Shown Using Absorbance Time (minutes) Absorbance of the M. Lysodeikticus standard containing 5.0 x 107 cells/ml 0 0.414 3 0.350 6 0.337 9 0.333 12 0.320 15 0.300 The longer the cells are left in the lysozyme solution, the less absorbance is shown. Discussion As noted in the discussion, lysozyme is more effective as a antimicrobial towards Gram-positive cells due to the larger amounts of peptidoglycan in the cell walls of these organisms. As can be seen here, lysozyme has a very distinct effect on the absorbance measurements from the M. lysodeikticus standard which increase substantially with time, suggesting that there are less viable cells the longer the organism is left in the presence of ...Download file to see next pagesRead More
Cite this document
  • APA
  • MLA
(“Bacterial cells Quantification Lab Report Example | Topics and Well Written Essays - 1000 words”, n.d.)
Retrieved from
(Bacterial Cells Quantification Lab Report Example | Topics and Well Written Essays - 1000 Words)
“Bacterial Cells Quantification Lab Report Example | Topics and Well Written Essays - 1000 Words”, n.d.
  • Cited: 0 times
Comments (0)
Click to create a comment or rate a document

CHECK THESE SAMPLES OF Bacterial cells Quantification

The Ethical Controversy of Stem Cells

As time goes by, people often think about what could be the next milestone for healthcare: Will doctors be able to cure cancer? Will cures be discovered for Parkinson’s disease, multiple sclerosis, and diabetes? Will we replace organs with mechanical man-made machines? It may surprise people but technology has made it possible to cure debilitating diseases as Parkinson’s disease, multiple sclerosis, diabetes; even the production of organs may not be far in the future considering these healthcare breakthroughs. These newly developing technologies could yield the biggest medical discovery yet. However, people are fighting against its discovery, and among them, even the President of the United States. This technology that...
11 Pages(2750 words)Term Paper

Antibiotic Resistance of Bacterial Agents of Disease

... amounts, and 10 ml will dispensed into 12 separate culture flasks. Twelve flasks will allow for duplication of cultures and blanks. A flask will contain 1 mg antioxidant/ml broth (but for epigallocatechin 0.1mg/ml). The control flask will have only MHB and no antioxidant. To prepare the planktonic cultures, an ml of overnight culture of bacteria will be grown in 10 ml of antioxidant enriched-MHB for 24 hours at 37°C, with shaking. After this period, 200 ul bacterial culture will be plated to selective MHA containing either fusidic acid or mupirocin. After 48 hours, the colony growth on selective media, and viable cells will be counted. S. aureus biofilms will be created using a Sorbarod apparatus as described previously (Muli...
7 Pages(1750 words)Research Proposal

Embryonic Stem Cells

...Embryonic Stem Cell Research: Science and Ethics Stem cell research is a polarizing political issue. It is difficult to separate scientific facts from the moral debate, even for scientists, because we have difficulty defining the point where the potentiality of cells has a good chance of becoming an actuality—in other words, when a pile of cells contains enough “life” to be something that should be protected, though it is not able yet to survive on its own. We carry our opinions of moral arguments around with us wherever we go, based on belief systems (whether scientific beliefs or religious beliefs). There is enough potential good in stem cell research that a well-thought, moral person can take a position in the debate, balancing science...
6 Pages(1500 words)Research Paper

Analysis of Bacterial Identification

... sections of the bacterial gene sample is hybridized with these probe. If one gets the hybridization, then the bactewria has been identifed accurately tracing back to the probe. This method is very expensive and cannot be used for general purposes. Mass spectroscopy with or without gas chromatography can be used to identify bacterial proteins or other metabolites, which can then be traced back to a species. For example muramic acid, found in the cell walls of most bacteria, is readily detected in the spinal fluids of patients with pneumococcal meningitis (15) or synovial fluids from septic arthritis (Fox, 2006) 5. Fully discuss, with examples of relevant situations, the significance of accurately identifying bacteria: Accurate...
6 Pages(1500 words)Assignment

Nanomaterials and their Applications in Solar Cells

...Nanomaterials And their Applications In Solar Cells Nanomaterials possess advantage in electric and optical properties, and when these carefully designed and fabricated nanostructures serve as a photo electrode film of solar cells, they can effectively improve the energy conversion efficiency by offering a large specific surface area, providing direct pathways for electron transport, or generating light scattering so as to extend the traveling distance of photons within the photo electrode film. Nowadays, the world consumes about 13 TW of continuous worldwide energy. By the year 2050, according to many energy experts we need about 30 TW to accommodate the increasing requirement of energy & economic growth. Moreover, this new energy has...
6 Pages(1500 words)Term Paper

Dye-Sentitised Solar Cells

... Contents 1.0 Introduction 4 2.0 Historical Background 7 3.0 Structural Components 9 3.1 Nanocrystalline Semiconductor Film Electrode 10 3.2 Dye Sensitizer 11 3.3 Electrolyte 18 4.0 Principle 20 5.0 Energetics of DSC 24 6.0 Present Research 26 6.1 Dye 26 6.2 Electrode 28 6.3 Electrolyte 31 7.0 Commercial Applications 32 7.0 Future Prospects 33 8.0 Conclusion 34 9.0 References: 35 List of Abbreviations PV Cells Photovoltaic cells CIGS Cu(In, Ga) Se2 or copper indium gallium selenide DSC Dye sensitized solar cells PEN Polyethylene tetraphthalate IPCE Incident photon to current conversion efficiency Bchlorine 1 Methyl trans 32 carboxy 8 deethyl 7 ethyl 8 oxo pyropheophorbide a Bchlorine 2 Methyl trans 32 carboxy 7 deethyl 8...
22 Pages(5500 words)Research Paper

How HeLa Cells Help Researchers Looking for Cancer Treatment

... How HeLa Cells Help Researchers Looking for Cancer Treatment Introduction Cancer has for a long time been among thetop global killer diseases among human beings. Church and Regis cite the search for immortality in human beings through an African American woman named Henrietta Lacks who died from cervical cancer on 4th October, 1951 at Johns Hopkins Hospital aged 31. Cell samples were taken from Henrietta’s cervix to be used for research purposes. These cells were code named HeLa cells, a name coined from the first two letters of her names. Before her death, a researcher from Hopkins named George Gey had observed that the cells could be grown in a glassware in the lab and kept alive for an indefinite period of time. The cells grew wildly...
8 Pages(2000 words)Literature review

Biotechnology-Microbial Fuel Cells

This project, therefore, aimed at establishing the voltage generated from three microbial species Shewanella oneidensis, Pseudomonas trivialis and Escherichia coli using H-type microbial fuel cells using synthetic wastewater as the substrate. E. coli generated the highest voltage and yielded a power density of 192.887 mW/m2 followed by S. oneidensis with 134.429 mW/m2 and least of all P. trivialis with 89.098 mW/m2. P. trivialis recorded the highest levels of COD removal (140%) followed by E. coli (133%) and finally S. oneidensis (44.08%). However, the coulombic efficiencies were lower than the literature values. These results meant that of the three microorganisms, E. coli was the best in voltage generation and that further studi...
21 Pages(5250 words)Lab Report

Microbiology and Chemistry, Bacterial and Fungal Infections

... of practical sessions, amoxicillin sodium and amoxicillin trihydrate, indicate which is used in which type of pharmaceutical preparation (1 mark). Amoxicillin sodium is used in the treatment of certain types of bacterial infection Amoxicillin trihydrate is used in the treatment or prevention of various types of bacterial infections 2. In Session 1, you determined the absorbance of a series of concentrations of amoxicillin sodium. Insert your spectra here. Insert your image as a jpeg in the box below, do not copy and paste the image as it may not resize correctly. Do not exceed this page – PTO 3. Complete the table below indicating the values for absorbance ‘A’ and the concentration in mM that were determined in Session 1. (6 marks...
6 Pages(1500 words)Assignment

Effect of Manuka Honey on the Proliferation of Human Breast Cancer Cells

...Effect of Manuka Honey on the Proliferation of Human Breast Cancer Cells Table of Contents Results Discussion Conclusion References Results On the T-test As mentioned, the T-test was used to ascertain how effective the Manuka honey was in the inhibition of the progression of the cancer cells of the breast. The findings are that increasing the concentration of the honey corresponded with the increase in the rate at which the breast cancer cells died, resulting correspondingly with the decrease in the amount of cancer cells that continued to proliferate after the exposure to the honey. The T-test on the other hand was employed to effect the comparison between the variables of honey concentration versus control. The gist of the t-test...
8 Pages(2000 words)Term Paper
sponsored ads
We use cookies to create the best experience for you. Keep on browsing if you are OK with that, or find out how to manage cookies.

Let us find you another Lab Report on topic Bacterial cells Quantification for FREE!

Contact Us