Contact Us
Sign In / Sign Up for FREE
Go to advanced search...

Development of a microfluidic device for extraction - Essay Example

Comments (0) Cite this document
Development of a microfluidic device for efficient protein extraction Eman Alzahrani* Department City * E-mail address: Abstract Sample pre-treatment is a required step in proteomics to remove interferences and concentrate the samples. A novel method to fabricate a continuous porous silica monolith inside a glass microchip and modify it with Octadecyl (C18) ligand was successfully developed…
Download full paperFile format: .doc, available for editing
GRAB THE BEST PAPER95.7% of users find it useful
Development of a microfluidic device for extraction
Read TextPreview

Extract of sample "Development of a microfluidic device for extraction"

Download file to see previous pages This newly microfluidic device for protein extraction may find an application in the area of proteomic research. Keywords: Microfluidic device; Sol-gel; Silica monolith; Protein extraction; Octadecyl (C18) 1. Introduction It is becoming increasingly important in the development of new medicines to use important a microfluidic tool for identifying proteins implicated in disease pathways. As the search for novel molecules to tackle diseases increases, the need to identify proteins on biological targets also increases. Efficient extraction of proteins is the most critical step for proteomics by removing the interfering materials and improving the detection sensitivity (Ahn & Wang, 2008). The recently invented silica monolithic materials are highly permeable to liquid flow and have high mass transport compared with the packed beds. Moreover, the monolithic stationary phase does not need frits, which can cause air bubbles to form and the proteins can be adsorbed into the frits and remain trapped (Cabrera et al., 2002 ). Fabrication silica monolith inside the microfluidic devices can decrease the volume of the sample and the reagents, and reduce the time of the analysis (Girault et al., 2004). Bienvenue et al. (2006) have observed that the negative aspect of the sol-gel monolith in microfluidic device is the fact that it shrinks while the monolith is formed. They further explain that this is can then cause the creation of an opening between the silica network and the microchip wall resulting in reduced surface area for protein adsoption. The aim of this contribution is to investigate the fabrication of a simple microfluidic device contained in a crack-free silica monolith to decrease sample handling, reduce contamination, be truly portable, and decrease analysis time. Moreover, its aim is to modify the surface of the silica monolith to Octadecyl silica (ODS) to use it for pre-concentration and extraction of proteins. 2. Materials and methods 2.1. Chemicals and materials Poly (ethylene oxide) (PEO) MW=10,000 Da, trimethylchlorosilane, tetramethylorthosilicate 99 % (TMOS), chlorodimethyloctadecylsilane 95 %, 2,6-lutidine 99 %, NaCl, and trizma base were purchased from Sigma Aldrich (Poole, UK) and used as received without any further purification. Bovine pancreas insulin, bovine heart cytochrome C, chicken egg white lysozyme, ?-lactoglobulin from milk bovine, haemoglobin from human, and bovine serum albumin (BSA) were purchased from the same. Nitric acid, ammonia, toluene, HPLC grade acetonitrile (ACN), and trifluoroacetic acid (TFA) was obtained from Fisher Scientific UK Ltd. (Loughborough, UK). MicroTight Adapter was purchased from Kinesis (Cambs, UK). Poly (ether ether ketone) (PEEK) tubing was purchased from Anachem (Luton, UK). 2.2. Instrumentation Baby bee syringe pump from Bioanalytical System Inc. (West Lafayette, USA). The instrument used for detection was HPLC-UV detection: 785A UV/Visible Detector from Perkin Elmer (California, USA). The reversed-phase analytical column was Symmetry C8 column, 4.6 mm ? 250 mm packed with silica particles (size 5 µm) from Thermo Fisher Scientific (Loughborough, UK). Scanning electron microscope (SEM) (EVO 60. Manufacturer: Carl Zeiss Ltd. (Welwyn Garden City, UK). SEMPREP 2 Sputter Coater from Nanotechnology Ltd. (Sandy, UK). 2.3. Fabrication of the silica-based ...Download file to see next pagesRead More
Cite this document
  • APA
  • MLA
(“Development of a microfluidic device for extraction Essay”, n.d.)
Retrieved from
(Development of a Microfluidic Device for Extraction Essay)
“Development of a Microfluidic Device for Extraction Essay”, n.d.
  • Cited: 0 times
Comments (0)
Click to create a comment or rate a document

CHECK THESE SAMPLES OF Development of a microfluidic device for extraction

Change Management Improving Training and Development

...CHANGE MANAGEMENT IMPROVING TRAINING & DEVELOPMENT TABLE OF CONTENTS Page 3 2 Diagnosis 4 3 Need for Change 5 4 Criteria for Change 5 4.1 Motivation 5 4.2 Creating a Vision 6 4.3 Developing Political Support 6 4.4 Managing Transition 7 4.5 Sustaining Momentum 7 5 Organisational Leadership 8 6 Human Relation Management 9 7 Recommendations 10 8 Bibliography 11 1 Abstract A diagnosis has been made of the situation at CF&F where there is a crisis due to high scale changes in both the top and middle management set-up. This has caused disruption and dissatisfaction both at the supervisory and worker levels. Strategic changes are necessary for retaining competitive advantage...
7 Pages(1750 words)Case Study

A Systematic Approach to Training and Development In Organic Juice Bar

 Prior to the discussion of training needs assessment, I would discuss several issues in the human resources management process that determine why training is important.
Daft and Fitzgerald (1992) indicate that in order to manage an organization effectively in a competitive environment, planning for human resources strategy requires several factors several external factors to be considered.

1. Attract an effective workforce. This includes planning on the number of employees required, job analysis or what types of jobs should be conducted or jobs associated with the types of business, forecasting future recruitment, recruiting because good employees need to be recruited, and selecting.
As stated by Daft an...
9 Pages(2250 words)Case Study

Management and Leadership Development

In modern epoch this theory does not apply, this is due to the fact that the education standards have got tremendously advanced and now – a – days the students are not taught the basic concepts of life because they ‘should’ know them, but rather the education standards and the curriculum is created in such a manner so that it embraces every aspect of the students' psychology and make the student think and act according to the way that he/she is being taught, rather than to just make him read the books over and over again. That is, education in the present era will build the student's skills and let the students gain knowledge rather than to just have them read, give their papers, and forget all about it.
8 Pages(2000 words)Case Study

Information Systems Development Process

When a project has an obvious objective to be attained and the effort is very small, common sense and attention to what the client wants can be a suitable alternative to more complicated and formal methods. However, when the software system becomes more complex, the objectives are multiplied and start to depend on different configurations and circumstances, it becomes imperative to structure the development effort to avoid software development that does not answer the clients need, or does not have the requisite level of quality, or becomes impossible to forecast in terms of time and budget – or a combination of any of the preceding.

In situations of complexity in software development, structure methods seek to im...
13 Pages(3250 words)Assignment

The Development of a Single Global Culture Will Benefit Humankind

Social relations show spatial transformation across the world, encompassing both the hazardous and beneficial effects of globalization (Held,2000) and are marked by the intensification of communication flows (Cochrane and Paine, 2004). Such flows as an increase in broadcast activities, satellite channels, Internet, telephony, people to people contact, migration, and proliferation of transnational companies are playing an important role in the development of a single global culture. There is an emergence of diasporic and shared cultural forms across the world that are aided by increasingly porous national boundaries.

According to Cochrane and Paine (2004), the lives of people in one part of the world are affected by even...
6 Pages(1500 words)Coursework

Leadership Development Experience

The nine-month certificate program which I completed at Leadership Institute of South Puget Sound combined academic study on leadership with practical development of leadership skills. Numerous community service activities such as involvement in Employee Community Fund (ECF), being a Child haven volunteer, Christian Center volunteer and a Junior Achievement Consultant have exposed me to situations which made me apply many skills such counseling young people, and motivating them to achieve their full potential, seeking solutions with expansive thinking and application of flexibility and quick responsiveness. My involvement in Christian Center had specially sharpened my crisis management skills by participating in natural disaster m...
13 Pages(3250 words)Assignment

Holistic Approaches to Infant Development 0-8 Years

see the child as like a seed that contains all the elements to produce a fruit if given the proper amounts of nutrients from the soil and water along with sunshine and an ideal climate (Brewer, 2001). The Constructivists, founded on the works of Jean Piaget and Lev Vygotsky, believe that children are not passive recipients of knowledge, rather, they actively work at organizing their experience into constructing their own learning.
The Early Years Foundation Stage (EYFS) is a resource for early childhood care and education practitioners to support the needs of young children under their care. It sets standards for learning, development and care for children up to five years of age. EYFS provides a wide variety of information o...
18 Pages(4500 words)Term Paper

The Role of Mass Media in the Development of Aggression

Despite the fact that longitudinal studies have proven that human aggression is to some extent an unwavering trait, it is undeniable that the environment plays a big role in influence the aforementioned behavior (Slaby & Roedell, 1982). Our daily encounter with the environment is a form of socialization that is defined as the process of integrating within oneself a sense of connection to a larger social world by means of discovering, understanding and adopting the beliefs, values, and norms of culture of other people. To a certain extent, sociologists consider the mass media as a powerful socializing agent as its significance is not constrained to the content alone of the media messages. Instead, it affects how we learn a...
10 Pages(2500 words)Report

The Effect of Democracy on Chinese Development

Any country first needs to go through economic liberalization and political liberalization before finally settling into a stable democratic situation. Democracy should not at any one point be viewed as if it were socialism, but should be seen as a means of promoting economic development through accountability and protection of individual lawful rights and interests...accountability in the sense that the respective leaders are able to fight corruption, enhance social harmony, strengthen public trust and more importantly empower government institutions that are directly in touch with the people (Liu, 2008, p.1-2). Owing to the Chinese well informed and affluent society on top of having a very dynamic economy, the political change wh...
7 Pages(1750 words)Coursework

Personal Rrofessional Development

Another challenge I faced was time management. I had limited time to study since I worked on a full-time basis. My poor time management skills worsened the matter for me. Indeed, this made me fail in my first presentation and essay despite starting work on it early enough. I had reserved two hours every day for my essay, as opposed to waiting until the last night before the presentation when I would be forced to work on it in a hurry. I also reserved time for my work-related tasks as well as relaxation in order to avoid exhaustion and boredom. However, I still failed. Still, on time management, I found it difficult to balance my time so as to have adequate time for each assignment. This could cause me to take up many tasks at the...
9 Pages(2250 words)Term Paper
sponsored ads
We use cookies to create the best experience for you. Keep on browsing if you are OK with that, or find out how to manage cookies.

Let us find you another Essay on topic Development of a microfluidic device for extraction for FREE!

Contact Us