The Concentration of Solutes Present in the Substances - Essay Example

It was used because to make a solution more concentrated, absorption of more light is required. To measure the calculations in the form of a graph, Beer-Lambert Law was used after having done the calculations. 

To start the process, 8mL of water was added to the 0.5M concentrated sample to produce a 0.2M solute. After this, 10mL of water was added to the sample to produce the 0.1M solvent. After these two steps, 10mL of water was also added to the sample to produce the 0.05M solvent. As the next step in the process, a test tube that had distilled water present in it was placed in Spec 20. The wavelength was also adjusted to know whether there is any problem with it or not.

In Spec 20, a 0.05M sample was placed to know its level of concentration, as well as percent transmittance. The process was repeated four times for the self-made solutions, as well as for the standard solutions after writing down the calculations. It was also repeated for the last time for the unknown solution. Tables 1 and 2 show the values that were recorded from the screen. Having done this, a graph was also developed to show the calculated absorption and concentration values.

It is important to mention that the graph was developed in excel and it depended on concentration vs. absorbance (Graph 1). Using the excel graph and the Beer-Lambert Law, the equation generated for the given points is as follows: Table 6 was drawn to reflect the difference between the absorbance rate of standard and self-made solutions.