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The author of this assignment "High Performance Liquid Chromatography" touches upon the method of separating and identifying the components of a mixture. It is stated that the chemical mixture is first dissolved in a mobile phase before passing through a stationary phase. …
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High Performance Liquid Chromatography
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Download file to see previous pages The illicit drugs sold on the streets are usually diluted with any material by the drug dealers who want to increase the quantity and street value of their drug products. The task of separating and identifying the components of the illicit drugs and is an arduous one and chromatography is applied in such cases (Allman 2010).
In both high-performance liquid chromatography and gas chromatography utilize columns, which are narrow tubes filled with the stationary phase, in which a mobile phase is passed. The chemical mixture is passed through the column by the continuous introduction of the mobile phase. This process is known as elution. The average rate that an analyte of interest is transported through a column depends on the time period it spends in the mobile phase (Sheffield Hallam University n.d).
The theory of chromatography is based on the observation that when dissolved in a solution or adsorbed on a solid surface, chemical substances partially escape to the surrounding environment. Hence the solubility of a gas in a liquid determines the distribution of the gas between a liquid phase and a solid phase. When the solubility of the gas is higher, the tendency of the gas to remain in the liquid phase is greater (Allman 2010). A component that is more soluble in the stationary phase will take longer the component that is less soluble in the stationary phase and more soluble in the mobile phase to travel through it (Sheffield Hallam University n.d). Distribution of analytes of interest between the mobile phase and the stationary phase can be described as follows where each analyte is in equilibrium between the two phases;
The partition coefficient is the equilibrium constant K and is defined as the analyte molar concentration in the stationary phase divided by the analyte molar concentration in the mobile phase.  ...Download file to see next pagesRead More
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