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Experiment to determine the presence of a single nucleotide polymorphism in a gene - Lab Report Example

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The variations occur whenever a single nucleotide (A, T, C and G) is differentiated between the biological species number or pairing chromosomes (Kwak, J. 2007). In many cases, the common SNPs have…
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Experiment to determine the presence of a single nucleotide polymorphism in a gene
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"Experiment to determine the presence of a single nucleotide polymorphism in a gene"

Download file to see previous pages Factors such as the recombination of genetics and the rate or mutation play a big role in determining the density of SNP (Kwak, J. 2007).
The density of SNP could be predicted by the microsatellites presence. The microsatellites AT nucleotides are the potent SNP predictors of density with the repeated long tracts that can be found in areas of reduced SNP density and low content of GC nucleotides. SNP in a population is assigned the frequency of a minor allele (Shastry, B. 2002). This involves the less frequency of allele for SNP. In order to understand the occurrence of a single nucleotide polymorphism, an experiment was set to determine the presence of a single nucleotide polymorphism in a gene.
The materials that were used in the experiment include: Sample 1, which was the Lambda DNA(λDNA) that was digested with Hindlll, Sample 2, which was a subjected DNA*PCR amplified for undigested WMIN gene, and sample 3, which was a subjected DNA * PCR amplified for the gene WMIN that was digested using Hindlll.
The loading buffer was added to the provided samples. The contents of the loading buffer are a tracking dye, bromophenol blue, and glycerol that made possible for the samples to sink into the well of loading on the gel. During the experiment, the disposable gloves were worn to reduce the contamination of DNAase from the fingers. About 50 mL 0.8% w/v agarose gel was prepared. Gelred was added to agarose gel (Su MC, Y. 2008). The agarose gel was placed into the apparatus of electrophoresis, having the wells on the black end of the cathode. TBE running buffer was added to cover the gel to a level of 1-2 mm over the surface. About 4µl quantity of samples having 100ng DNA was loaded from sample one to three inside the wells using P20 Gilson. The lid was put on, the power turned on and adjusted to about 100 v. The power was run for approximately 30 minutes up to when the blue tracker dye was about ¾ of the gel. The power supply was turned off and the ...Download file to see next pagesRead More
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