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Uv/vis - Lab Report Example

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A qualitative analysis on the performance characteristics of a UV/VIS absorption spectrometer is important in accessing how this…
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Download file to see previous pages In this experiment, there was need to introduce the students to how the UV/VIS applies in the laboratory. It involved studying how the adjacent and visible lights can help in determining the concentration of given ions in solutions. A qualitative analysis on the performance characteristics of a UV/VIS absorption spectrometer was also an important objective of this experiment. The analysis would help in providing more information about the mechanism of the concept in determining the concentration of ions. The third objective of the experiment was to apply the significant characteristics of UV/VIS absorption spectroscopy in real scenario. This involved determining concentrations of the CO2+ and Cr3- in a sample of solution through the help of this concept.
In order to achieve the above objectives, there was need to apply in Perkin Elmer Lambda 35 UV/VIS spectrometer in combination with other apparatus to collect data. Perfoming a qualitative analysis involved acquiring and analyzing spectra of various substances under different conditions. Different instrumental parameters were also used as a modification for the experiment of measuring spectra of different optical materials. In the second part involving simul analysis of a two-component mixture using the spectroscopy method, two light-absorbing ions (Cr2+ and Cr3+) were used. A solution containing both of the ions was presented for analysis. The preference of these ions resulted because of they cannot interfere with the light-absorbing properties of each other. This means that they allow for the summation of their absorbance properties. This experiment worked under the principle: the total absorbance at any given wavelength is equal to the sum of the absorbance that the two components would show if measured individually under the same conditions. Further, the beer lambert law was also considered to help in determining the concentration of the unknown solutions. in ...Download file to see next pagesRead More
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