Nobody downloaded yet

Raman Spectroscopy of Toluene and Fluorescence Spectroscopy of Rhodamine 6G - Lab Report Example

Comments (0) Cite this document
For a transition to be Raman active there must be a change in polarizability of the molecule. Polarizability is the change of the dipole moment with distance. Raman spectroscopy involves the…
Download full paperFile format: .doc, available for editing
GRAB THE BEST PAPER96.6% of users find it useful
Raman Spectroscopy of Toluene and Fluorescence Spectroscopy of Rhodamine 6G
Read TextPreview

Extract of sample
"Raman Spectroscopy of Toluene and Fluorescence Spectroscopy of Rhodamine 6G"

Download file to see previous pages In Raman spectroscopy, light and matter interacts. Both IR and Raman vibrational bands are described by their frequency (i.e energy), band shape, and intensity.
At low frequencies, the fingerprint region is found for most compounds. Raman spectroscopy is much easier to use at low frequencies compared to IR, this is because at low frequencies very many bands are visible on the spectrum. With Raman spectroscopy, it is possible to reject the laser beam at low frequency modes; this would minimize the bands that are seen at the fingerprint region. With IR, this is not possible, and as a result the spectrum cannot be refined to reduce the congestion of peaks.
Toluene (methylbenzene) has a CH3 group attached to the benzene ring, for IR spectroscopy, the CH3 group exists at just below 1500 cm-1. This is just at the start of the fingerprint region; therefore Raman spectroscopy would be much better at elucidating its structure over IR.
Fluorescence is a spectrochemical method of analysis where the molecules of the analyte are excited by irradiation at a certain wavelength and emit radiation of a different wavelength. The emission spectrum provides information for both qualitative and quantitative analysis. When light of an appropriate wavelength is absorbed by a molecule (i.e., excitation), the electronic state of the molecule changes from the ground state to one of many vibrational levels in one of the excited electronic states. The excited electronic state is usually the first excited singlet state, S1 (Figure 1). Once the molecule is in this excited state, relaxation can occur through several processes. Fluorescence is one of these processes and results in the emission of light.
Fluorescence corresponds to the relaxation of the molecule from the singlet excited state to the singlet ground state with emission of light. Fluorescence has short lifetime (~10-8 sec) so that in many molecules it can compete favorably with collision deactivation, intersystem crossing and ...Download file to see next pagesRead More
Cite this document
  • APA
  • MLA
(“Raman Spectroscopy of Toluene and Fluorescence Spectroscopy of Lab Report”, n.d.)
Retrieved de
(Raman Spectroscopy of Toluene and Fluorescence Spectroscopy of Lab Report)
“Raman Spectroscopy of Toluene and Fluorescence Spectroscopy of Lab Report”, n.d.
  • Cited: 0 times
Comments (0)
Click to create a comment or rate a document
Absorption Spectroscopy
Comparing it with the LD50 (6-10g/kg body weight), it was found that the level of Allura Red dye in the water is within normal levels. Absorption spectroscopy is an important analytical tool that is
4 Pages(1000 words)Lab Report
Evaluation of Three Different Types of Spectroscopy
atoms and amount of light absorbed, therefore, we can determine the concentration of the sample by measuring the amount of light absorbed (Karabegov, 2011). Samples have to be heated to extremely high temperatures in other to atomise them. The sample goes through a nebulizer
2 Pages(500 words)Lab Report
Molecular Fluorescence Analysis of Proteins
Fluorescence occurs when an excited molecule goes back to the ground state by photon emanation. Conversely, phosphorescence occurs when the excited electron
4 Pages(1000 words)Lab Report
For reactions occurring in solutions, the solvent exerts an effect as a consequence of it being a condensed phase, with its molecules very close together even in case of a chemically inert medium (Arnaut, Formosinho and Burrows 225). In fluorescence quenching, a reaction takes
2 Pages(500 words)Lab Report
Physical Chemistry Lab Report
Brass is a widely used alloy comprising mainly of copper and zinc. In an effort to enhance certain properties of the brass alloy, traces of
7 Pages(1750 words)Lab Report
According to Lardan H. understanding ‘Series of waves converges to series limit at an air wavelength of 364.60 mm …’ (Lardan, 1998, pp. 49 -53) clearly reveals that the
3 Pages(750 words)Lab Report
Distillation and Gas Chromatography: Separation of Hexanes and Toluene
The methods utilized in the experiment involved precise raising and falling of temperatures at specific temperature ranges to obtain three
4 Pages(1000 words)Lab Report
Immuno-labelling, fluorescence microscopy and live time-lapse imaging
The detection of the antibody reaction with the specific antigens is examined and all the physiological process behind it. The experimentation processes of immune-imaging of the cells on the photon of the fluorescent microscopy are also explored in the paper.
8 Pages(2000 words)Lab Report
Infrared spectroscopy structural determination
It seeks to classify and research on chemicals. A lab instrument that is used to carry out this research is the Fourier transform infrared spectrometer. The Fourier transform Infrared spectrometer gives an electromagnetic spectrum that is
1 Pages(250 words)Lab Report
Scientific Investigation
search study’s main hypothesis is that a combination of DNA shuffling and either selection or screening would be able to optimize combining positive mutations and simultaneously removing negative mutations from the sequence pool, thus improving fluorescence. In order to test
2 Pages(500 words)Lab Report
sponsored ads
We use cookies to create the best experience for you. Keep on browsing if you are OK with that, or find out how to manage cookies.
Let us find you another Lab Report on topic Raman Spectroscopy of Toluene and Fluorescence Spectroscopy of Rhodamine 6G for FREE!
logo footer
Contact us:
Contact Us Now
FREE Mobile Apps:
  • StudentShare App Store
  • StudentShare Google play
  • About StudentShare
  • Testimonials
  • FAQ
  • Blog
  • Free Essays
  • New Essays
  • Essays
  • Miscellaneous
  • The Newest Essay Topics
  • Index samples by all dates
Join us:
Contact Us