Retrieved from https://studentshare.org/chemistry/1585111-adsorptiondesorption-of-chlorohexidine-chx-on-flax-and-cotton-fibers
https://studentshare.org/chemistry/1585111-adsorptiondesorption-of-chlorohexidine-chx-on-flax-and-cotton-fibers.
Compared to previous results, changes and trends in absorbance are easier to recognize now with 30% ethanol concentration in the solvent. It is very clear in Figure 1 that the difference in light-absorbance in equilibrium compared to the initial is greater for flax than for cotton. This simply implies that less chlorhexidine was left in the solution with flax than in cotton due to the fact that flax has adsorbed more chlorhexidine than cotton. It appears that flax can adsorb almost twice the amount of chlorhexidine cotton can adsorb.
This suggests that flax has better potential as antiseptic carrier of chlorhexidine. Figure 1. Light-absorbance profile of flax and cotton in 0.017mg/ml Chlorhexidine. These remarks on the results, however, are mere eyeball observations. It is essential that the results be statistically analyzed to properly prove that the type of fiber really has significant effect on light-absorbance and adsorption of chlorhexidine. Since there are two-variables being analyzed—type of fiber and time, it is appropriate to use two-way analysis of variance (ANOVA).
Table 1 shows the values calculated. If the F value computed is greater than the critical value (F crit) and the P-value is less than 0.05, then the difference between attributes are significant. Some scientists determine the level of significance of the result with the p-value. Some consider that p-values lower than 0.001 signifies very significant results.Table 1. Statistical Analysis: Two-way Analysis of VarianceSource of VariationSSdfMSFP-valueF critType of Fiber1.1747211.17472127421.53.49E-594.
149097Time1.62792670.23256125225.85.38E-582.312741Interaction0.17252570.0246462673.3842.06E-422.312741Within0.000295329.22E-06Total2.97546647 First row of Table 1 shows that the F value of 127421.5 is exceedingly greater than the critical value of 4.149097. Also the P-value is remarkably low. This suggests that the type of adsorbing fiber extremely affects the adsorption of chlorhexidine. The second row means that adsorption is also very much influenced by the reaction time. And the third row confirms that there is interaction between time and type of fiber.
Simply put, both time and type of fiber affects adsorption such that the cause of changes in chlorhexidine adsorption cannot distinctively be attributed to just one factor. It can be observed in Figure 1 that the change in absorbance for the first 10 minutes is very steep compared to the changes at later times. One-way analysis of variance of flax results, as shown in Tables 2 and 3, suggests that change in absorbance are significant until after 50 minutes of flax submersion in chlorhexidine.
This simply means that flax only reached equilibrium after 50 minutes. Table 4 and 5, on the other hand, proves that equilibrium was attained within 30 minutes by cotton. This implies that cotton achieves equilibrium faster than flax.AppendixStatistical AnalysisTable 2. One-way ANOVA of absorbance for flax samples from 40 to 60 minutes.Anova: Single FactorSUMMARYGroupsCountSumAverageVariance4030.44030.1467672.72E-065030.41630.1387671.43E-076030.41360.1378671.49E-05ANOVASource of VariationSSdfMSFP-valueF critBetween Groups0.00014427.2E-0512.163850.0077435.
143253Within Groups3.55E-0565.92E-06Total0.000188 Table 3. One-way ANOVA of absorbance for flax samples from 50 to 60 minutes.Anova: Single FactorSUMMARYGroupsCountSumAverageVariance5030.41630.1387671.43E-076030.41360.1378671.49E-05ANOVASource of VariationSSdfMSFP-valueF critBetween Groups1.22E-0611.22E-060.1616050.7082377.708647Within Groups3.01E-0547.52E-06Total3.13E-055 Table 4. One-way ANOVA of absorbance for cotton samples from 20 to 60 minutes.Anova: Single FactorSUMMARYGroupsCountSumAverageVariance2031.59110.5303678.
23E-073031.57370.5245679.96E-064031.56010.5200331.37E-055031.55040.51684E-056031.54520.5150671.9E-05ANOVASource of VariationSSdfMSFP-valueF critBetween Groups0.00046140.0001156.892930.0062363.47805Within Groups0.000167101.67E-05Total0.00062814 Table 5. One-way ANOVA of absorbance for cotton samples from 30 to 60 minutes.Anova: Single FactorSUMMARYGroupsCountSumAverageVariance3031.57370.5245679.96E-064031.56010.5200331.37E-055031.55040.51684E-056031.54520.5150671.9E-05ANOVASource of VariationSSdfMSFP-valueF critBetween Groups0.00015735.23E-052.5296050.1307364.
066181Within Groups0.00016582.07E-05Total0.00032211
Read More