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Processes for the study of tissues - Lab Report Example

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This report discusses three methods for the study of tissues to carry out fixation which includes^ heat, immersion, perfusion and also clearing, embedding, section cutting. Also, the final step discusses staining is carried out in order to give a contrast and emphasize important features…
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"Processes for the study of tissues"

Download file to see previous pages i) For hardening to occur it is necessary to dehydrate the tissue as paraffin wax used as an embedding substance is immiscible in water. Dehydration is done by passing the tissue sample through very high concentrations of ethanol. ii) Histo-clear is a clearing substance which offers rapid clearing at a low cost. It has also offered a way out of the adverse effects of breathing xylene while preparing slides. It also offers the tissue the right amount of hardness and produces better staining.iii) Unstained tissues lack inherent contrast and thus it is almost impossible to clearly view them in any microscope, be it light or electron.iv) Nuclei are stained blue while cytoplasm is stained pink) Periodic Acid-Schiff stain is used to detect different carbohydrates. Periodic acid oxidizes diol group forming aldehydes which on reaction with Schiff stain gives purple-magenta color. Duodenum contains an abundance of goblet cells which have a very high percentage of mucus containing glycoprotein. This will results in a purple-magenta color on staining with periodic acid Schiff stain.At any instance, the person should be respected whether the research is for new medical treatment or studying people habits. The autonomy should be foremost respected. Research should maximize benefits and minimize harms. In addition to this independent panels should be established who shall first review the research methodology and judge the benefits and harms to humans. Most importantly any kind of deception should be avoided at all costs. ...Download file to see next pagesRead More
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