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Summary - Admission/Application Essay Example

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The two articles on yeast fermentation and bio-fuel production are entitled “Altered sterol composition renders yeast thermotolerant” by Caspeta et al. and a second one is that of “Engineering alcohol tolerance in yeast” by Lam et al. Both are published in October 3,…
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Download file to see previous pages One viable alternative is biofuel which is fuel derived from plants or crops. It involves a chemical process by which the sugars contained in plants are converted to ethanol. The primary agent in this process is yeast to cause fermentation or the way by which a plants sugars (mainly glucose) in the cellulose or starch are converted by the anaerobic process into ethanol (a form of alcohol) by the action of yeast enzymes. However, the yeast enyzmes are often susceptible and very sensitive to ethanol toxicity that limits industrial production.
The research study done by Caspeta et al. was intended to solve this problem by use and selection of specific yeast strains through laboratory evolution that are tolerant at the ≥40°C temperature at which ethanol production is maximized through both saccharification and fermentation processes. The research study authors experimented by sequencing the whole genome, studying genome-wide expressions, and by analyzing the metabolic-flux by which they discovered a change in sterol composition, from ergosterol to that of fecosterol.
The major findings of their study were the change in sterol composition was caused by mutations in the C-5 sterol desaturase gene and that large chromosome III rearrangements and mutations due to DNA damage and respiration did not significantlly contribute to the rise or development of any new thermotolerant phenotypes of yeast (Caspeta et al., ...Download file to see next pagesRead More
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