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Detection of Organic Molecules in Unknowns - Lab Report Example

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The Beer Lambert’s law illustrates that the amount of light conveyed through a solution is related to the concentration of the substance (“Beer-Lambert Law”). Therefore, the concentration of substances can be determined by measuring the amount of absorbed light using…
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Detection of Organic Molecules in Unknowns
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Download file to see previous pages The absorbance values of the three unknown samples were also determined and recorded.
Figure 1: Calibration curve for the standard solutions. The absorbance of the standard solutions was plotted against the concentrations in µl/ml. The best line of fit and the equation of the line were also determined.
It was observed that the absorbance of the solutions increased with an increase in the concentrations. Substitution of the absorbance values of the unknown into the equation of the standard curve enabled the determination of the concentrations of the unknown samples. Therefore, it was concluded that spectrophotometry is a reliable and efficient method of determining the concentrations of unknown substances provided that a calibration curve is ...Download file to see next pagesRead More
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