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Per3 Gene and Diurnal Preference - Lab Report Example

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In the paper “Per3 Gene and Diurnal Preference” the researcher provides an experiment, where 50 individuals were selected for the study and they were asked to rinse their mouth with 12ml of 0.9% saline and the second spit was collected in the falcon tubes…
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Per3 Gene and Diurnal Preference
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"Per3 Gene and Diurnal Preference"

Download file to see previous pages The forward and reverse primers were chosen for PER3 gene and they were added to the PCR tubes along with crude DNA, polymerase buffer, Taq polymerase and DNTPs.  Forward and reverse primers are chosen based on the sequence of the PER3 gene. The primers must have the unique sequence region of each gene that is not present in any other gene.  The chance for primer – dimer formation is high because of many reasons. The annealing temperature may not be correct or the template may not be proper or the DNA may have contaminants. To prevent the primer – dimer formation, the above points must be taken care off. DNA polymerase buffer helps to increase the dNTPs binding with the DNA template. Taq polymerase enzyme polymerizes double stranded DNA formation. It acts as the catalyst for the amplification reaction.The master mix was prepared according to the instructions. The tubes were then placed in the PCR and the amplification was performed. To check the success of PCR amplification, the amplified PCR product was run in the agarose gel electrophoresis. 1.2% agarose gel was preared and casted in the casting tray. The electrophoresis box was filled with the electrophoresis running buffer and the DNA products were loaded in the gel with the loading dye. DNA ladder was loaded in the first lane and the samples in the other lanes. The positive control was a readily avalilable one and the negative control was distilled water. The postive control enables us to make sure that the separation of the DNA bands. ...Download file to see next pagesRead More
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