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Biology - Essay Example

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These tumor cell lines are important resources for understanding cancer stages. The cell lines of colorectal carcinoma such as CACO-2, CoCM-1, Colo 201, DLD – 1, H630, HCT 116, HT…
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Download file to see previous pages HCT 116 cell lines belong to the DNA repairing community and nearly 22 genes were disrupted in this cell line. Most of these genes are similar to the genes disrupted by other cell lines. Gene targeting is highly successful in this cell line and it is the only cell line without knock out strategies. HCT 116 cell lines can also be used for gene knock out in somatic cells. The targeting frequency of HCT 116 is 37% more than other cell lines for the p21 loci. (Conn 2008). LOVO cell lines are derived from the metastatic site of the colon. It is the best transfection host for cancer studies.
The Colorectal Cancer cells are aneuploid cells which undergo a large-scale chromosomal rearrangement. LOVO and LS-174T cell lines have KRAS mutations. LOVO lacks BAT- 26 locus. (Ahmed et al. 2013). According to Bu et al (2011), the order of efficiency of cell lines is given as Caco-2> HT – 29 > LS174T > LOVO. LS174T cell line is best used for the investigation of the phenotypes. Similarly, proliferative and invasive capacity of LS174T is very high than any other Colorectal Cancer cell line. Thus LS174T can be used for the study of the mucin expression in the colon cancer. (Bu et al. 2011). Bcl – 3 ( B –cell lymphoma 3 – encoded protein ) is an oncogene protein. It is a regulator present in the NF- kB. The studies on Bcl- 3 have found that Bcl- 3 is an important oncogenic regulator for the study of Colorectal Cancer. Bcl- 3 is found to activate the homodimers such as p52 and p50. The phosphorylation of Bcl-3 results in the up regulation. This is very important for the colorectal carcinoma. (Puvvada et al. 2010).
CAECAM (Carcinoembryonic Antigen- related Cell Adhesion Molecule) belongs to a group of Carcinoembryonic antigen family (CEA). CEA contains two sub groups: CAECAM and Pregnancy-specific glycoproteins (PSG). CAECAM are found to a tumor suppressoe molecule by down regualting the growth and ...Download file to see next pagesRead More
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