The present report entitled "Regulatory Genes" explores how regulatory genes direct vertebrate development. It is stated here that the Lhx3, a homeodomain transcription factor is known to be important in the formation of pituitary gland and anterior pituitary secreting cell type’s specification…
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For the second experiment, the mutant and wild type cDNA was constructed. The cDNAs and GST genes were inserted into an expression vector. This stage was carried out to synthesize fusion proteins of LHX3a, LHX3bY116C, LHX3aY111C and LHX3b all fused to GST. Glutathione S-transferase (GST) is known to be a protein which aids in the purification and isolation of proteins of interest. The controls were set up by isolating the GST proteins only. The fusion proteins were later separated with the use of electrophoresis and the proteins were used as a substrate for the binding assay. Purified radio-labeled Pit-1 and NIL were later added to the gel that had the mutant and wild type fusion proteins.In this case, if there were binding sites(No mutation effect), the Pit-1 and NIL proteins could bind to the proteins(LHX3).The presence of the bound Pit-1 and NIL were visualized with fluorography. The binding of Pit-1 and NIL were then quantified by scintillation counting (electrophoresis gel band intensity measurement).The first experiment aimed at determining if mutations in the LXH3 gene can interfere with LHX3a and LHX3b’s ability to binding to DNA promoters and reporter genes. As indicated in figure 1, both mutation types affected the activation of alpha GSU gene (red bars). The truncated mutations with no DNA binding domain were not able to activate the reporter gene, while on the other hand, point mutations with altered amino acid in the LHX3 protein interaction domain, had reduced activation.
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ROCK2, ACE, NOS3, H19, COMT, and STOX1 are all discussed as possibilities, though only H19 showed any serious promise as a causal link. Genes related to preeclampsia are more likely to be those related to cardiovascular function than to fetal growth or uterine health.
The bacterium is saprophytic, do not form spores and lives in usually lives in soil. The bacterium has capabilities of infecting both humans and plants via open wounds, inhalation and ingestion. The bacterium doesn’t have a definite incubation period but
Intuitively, this other method involves the formation of an amide then cyclizing it to yield α-carbolines even though it is always a poor yield. Despite limited success associated with the process, the reactions involve proved to be better as supported by
Even though there exist no certainty that a gene recombinant product will be accumulated in the Escherichia coli (E coli) at levels that are high, in biologically active foam, and in full length, a significant magnitude of efforts has been put towards bettering the versatility and performance of the microorganism.
In actual sense, the recipient will have the same combination of genes that are used in the plasmid. this is a type of combining genes from the donor to the recipient, in making a strong production. For instance, during the 1970’s researchers had started the research on how genes could be combined from a donor to the recipient.
This report examined the features of E coli in respect to the expression systems with an emphasis on the limitation number that have been addressed. For a high gene dosage to be achieved, the heterologous cDNAs are normally cloned into replicate plasmids in a fashion that is relaxed and are normally existence at 15-60 copies per cell.
The paper concludes that theoretical probability sufficiently predicts inheritance of genes.
Olofsson explains that the knowledge of probability is fundamental in understanding inheritance of genes (p. 56). This is because characteristics of
Human papilloma virus (HPV) 18-specific primers were used to amplify viral DNA. After 30 PCR cycles, the PCR products were subjected to agarose gel electrophoresis (AGE). Results showed that the genomic DNA of HeLa cells is contaminated with HPV-18 DNA
Adenine pairs with thymine while cytosine pairs with guanine. DNA was discovered back in 1868 by a Swiss physician, Friedrich Miescher who isolated “nuclein” a nuclei of cells which is today referred as
About Genes. DNA is the basic form in which instructions and information on the characteristics of any organism is stored in the cells. This information and instructions for the synthesis of the proteins is stored in DNA molecule in form of a sequence of nucleotides, including Adenine, Thymine, guanine and cytosine; commonly abbreviated as A, T, G and C respectively, in a given order.
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