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Biochemical identification-qualitative analysis - Lab Report Example

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In the experiment, present of calcium ions, chloride ions, reducing sugars, polysaccharides, lipids, carbohydrates, amino acids, nucleic acids and peptides tests were done using various methods…
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Biochemical identification-qualitative analysis
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Download file to see previous pages Thus it is paramount to be able to determine the specific elements present in a given compound. As a result, several methods have been devised to facilitate the detection of the different elements present in various selected compound. This not only helps detect the elements present, but also the minerals and the type of ions present.
10 drops of 2% calcium chloride, yoghurt extract and water were put in the first, second and third test tube respectively. The three test tubes were then labelled clearly using a pencil and a sticker. 10 drops of oxalic acid were then added in each of the tubes and the solution shaken thoroughly. The results were then recorded in a table form.
10 drops of 2% calcium chloride, yoghurt extract and water were put in the first, second and third test tube respectively. The three textures were then labelled clearly using a wax pencil. 10 drops of silver nitrate were then added in each of the three test tubes and the results recorded in form of a table.
20 drops of each of lactose, glucose, starch, Hydrolysed glucose, hydrolysed starch and water were put in a test tube. The tubes were then clearly labelled at the rim. Benedict’s reagent was then added to each of the tube while at the same time mixing by shaking gently. The test tube were the heated under a water bath for two minutes after which they were removed and the results recorded in a table.
The gridded white paper was labelled with hydrolysed starch, starch, glucose, lactose, sucrose and hydrolysed sucrose. Water was also used as a control experiment. A spot plate was put on the label white paper and few drops of each of the above stated reagents added to the corresponding wells on the spot. 1 drop of I2 – KI was then added to each sample and mixed thoroughly with a tooth pick using different toothpick on each reagent spot. The results were then recorded in a table.
The reagents Nucleic Acid, Amino ...Download file to see next pagesRead More
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