quora
Nobody downloaded yet

Nitric Oxide Production in Cytokine Activated Macrophages - Lab Report Example

Comments (0) Cite this document
Summary
Microphages play a key role in the regulation of adaptive and innate immune responses via intracellular and phagocytosis digestion of apoptotic cells, microorganisms, and inflammatory byproducts, and the secretion of chemokine, cytokines, and reaction nitrogen and oxygen…
Download full paperFile format: .doc, available for editing
GRAB THE BEST PAPER93.6% of users find it useful
Nitric Oxide Production in Cytokine Activated Macrophages
Read TextPreview

Extract of sample
"Nitric Oxide Production in Cytokine Activated Macrophages"

Download file to see previous pages Apoptosis plays an important role in maintaining homeostasis in a number of physiological systems. NO can cause apoptosis to be induced in various cells including immune cells and tumor cells. Excessive and prolonged production of NO can trigger secondary damage of normal tissues. Therefore, the NO-secreting and activate macrophages needs to die or revert back to a resting state in order to prevent damaging of normal tissues (Tsujimoto, & Shimizu, 2005). Activation – induced apoptosis is the mechanism involving the removal of immunologically stimulated cells to help limit injury of normal tissues and to promote immunologic tolerance. IL-2, one of the major cytokine, which is secreted from an activated lymphocyte during inflammatory or immune responses, can trigger various immune cells such as macrophages and lymphocytes to produce a plethora of cytokines including TNF-β, TNF-α, IL-1β, IL-6, and IL-1α. (Tsujimoto, & Shimizu, 2005). These cytokines can potentially induce responding cells for instance macrophages to synthesize and produce high NO nitrite levels from l-arginine through the action of the enzyme called inducible nitric oxide synthase. In this study, the focus was ascertaining the impact of the cytokines on the rate of synthesis and production of NO from macrophages (Hu, & Brindle, 2005).
In relating the optical density to the levels of nitrite, volumes of standardized sodium nitrite solution 50µg/ml) was added to culture medium. It was observed that increase in the volume of Volume of 50µg/ml standard added to 0.5ml culture medium caused a corresponding increase in Optical density (OD) as depicted in table 01 and graph 01.
The results of this experiment indicate that cytokines have an impact on the production of NO by macrophages. The higher the number of cytokines used the higher the Optical density values hence the higher production of NO. The value for OD was lowest (OD=0.057) ...Download file to see next pagesRead More
Cite this document
  • APA
  • MLA
  • CHICAGO
(“Nitric Oxide Production in Cytokine Activated Macrophages Lab Report”, n.d.)
Retrieved from https://studentshare.org/biology/1631761-nitric-oxide-production-in-cytokine-activated-macrophages
(Nitric Oxide Production in Cytokine Activated Macrophages Lab Report)
https://studentshare.org/biology/1631761-nitric-oxide-production-in-cytokine-activated-macrophages.
“Nitric Oxide Production in Cytokine Activated Macrophages Lab Report”, n.d. https://studentshare.org/biology/1631761-nitric-oxide-production-in-cytokine-activated-macrophages.
  • Cited: 0 times
Comments (0)
Click to create a comment or rate a document
CHECK THESE SAMPLES - THEY ALSO FIT YOUR TOPIC
Lab report 3
The polyphenols in tea are known to have antioxidant properties which can quench free radical species, and also pro-oxidant activities that appear to be responsible for the induction of apoptosis in tumor cells. The bioavailability of these natural compounds is an important factor that determines their efficacy.
12 Pages(3000 words)Lab Report
Microbiology
Using this model, we aim to understand the extent of change in the GI tract microbial count of streptozotocin injected diabetic rats compared to in normal rats based on a comparative assay of their glucose content, lactate content and the ratio of the GI tract to the body weights.
30 Pages(7500 words)Lab Report
Lab report (Should we mine this ore)
For instance, the metal of interest was copper and its amount in the ore sample taken from the site was to be determined. Chemists usually analyze the rock samples to find out if the underground deposits are economically feasible
3 Pages(750 words)Lab Report
Empirical Formula of Magnesium Oxide

The author states that the formula of Magnesium can be predicted with the use of atomic theory, which leads to the electron structure of Magnesium and Oxygen atoms. The aim of the experiment is to compare the empirical formula of Magnesium Oxide developed after the experiment with the formula developed during the experiment.

6 Pages(1500 words)Lab Report
Biology BSc third year biotech project; Manipulating Biomass and PHA Production in batch and continuous cultures of Paracoccus denitrificans
the amount of protein produced for substrate succinate was estimated and found to increase with the increase in the concentration of the succinate concentration. The protein production was found to be maximum for the succinate concentration of 50 mM. When the concentration of
14 Pages(3500 words)Lab Report
Peptidoglycan and Lipoteichoic Acidinduced Cell Activation
When certain bacterial types invade an organism they bind to the host's cell membranes stimulating a cascade of events eventually resulting in NFB activation, its translocation to the nucleus and corresponding cytokine release and activity within the bloodstream.
10 Pages(2500 words)Lab Report
Interferences in the Assay of Serum Albumin Using the Bromcresol Green Indicator
In clinical medical laboratories the testing for serum albumin, globulins and total protein are standard procedures, performed daily. Theses assays may also be requested STAT or on an emergency basis for patients suffering a crisis situation. The techniques for determining albumin and total protein are numerous and varied.
10 Pages(2500 words)Lab Report
Conservation of mass during a chemical change
In the first set up, sodium carbonate was reacted with calcium chloride. From our knowledge of chemical reactions we know that when you react the two the
5 Pages(1250 words)Lab Report
Application of Microscopy in Biomedical Sciences
Fluorescence emission in the fluorescence microscopy is not only characterized by the position and intensity but also the wavelength, lifetime and polarization. Use of fluorescence imaging has made it easier the observation of photo physical events. Rotation and mobility of the fluorescence is measured through the TR-FAIM as “Time-resolved fluorescence anisotropy imaging”.
6 Pages(1500 words)Lab Report
sponsored ads
We use cookies to create the best experience for you. Keep on browsing if you are OK with that, or find out how to manage cookies.
Let us find you another Lab Report on topic Nitric Oxide Production in Cytokine Activated Macrophages for FREE!
logo footer
Contact us:
+16312120006
Contact Us Now
FREE Mobile Apps:
  • StudentShare App Store
  • StudentShare Google play
  • About StudentShare
  • Testimonials
  • FAQ
  • Blog
  • Free Essays
  • New Essays
  • Essays
  • Miscellaneous
  • The Newest Essay Topics
  • Index samples by all dates
Join us:
Contact Us