quora
Nobody downloaded yet

Cell cycle by emphasizing DNA replication - Research Paper Example

Comments (0) Cite this document
Summary
Isolation and characterization of dominant negative mutant alleles of CDC6 done to comprehend extensively the importance and regulation of CDC6 in DNA replication, CDC6 gene containing plasmid mutagenized randomly under GALI 10 promoter by passage through E. Coli strain, The…
Download full paperFile format: .doc, available for editing
GRAB THE BEST PAPER98.2% of users find it useful
Cell cycle by emphasizing DNA replication
Read TextPreview

Extract of sample
"Cell cycle by emphasizing DNA replication"

Download file to see previous pages Increase in the number of cells with 2C-DNA content attests that over expression of wild type CDC6 impacts on the cell cycle kinetics. However, this over expression has no effect on cell proliferation. After S phase is complete, CDC6-d2 on the other hand blocks cell cycle progression and inhibits proliferation as cells with 2C DNA pile up; CDC6-d2 does not inhibit DNA replication but it does passage via mitosis.
When SCF CDC4 is inactive, dephosphorylation of B subunit can be driven by over expression of the wild type CDC6 in nocodazole arrested cells. This however is not the case as with when the SCF CDC4 is active as over expression of the cells does not lead to dephosphorylation of the B subunit because over expression of CDC6-d2 blocks cells in G2/M by inactivating CDC28-Clb kinase and in a CDC4+ background produces phenotype very much like that produced by the over expression of wild type CDC6 in a CDC4 mutant background.
Unlike CDC6-d2 which is stable in nocodazole arrested cells, the wild type protein is not and quickly disappears into the cells thus showing CDC6-d2 as being resistant to mode 3 proteolysis. It is noted however that CDC6-d2 is not affected for mode 1 proteolysis or mode 2 proteolysis but is affected as a substrate for mode 3 proteolysis; a single point mutation yielding transformation from C to T at bp 1103 causing substitution of threonine at amino acid 368 with methionine was pointed out after DNA analysis of the CDC6-d2. There are three glycine and six proline residues in a 50 amino acid residue surrounding this region, triggering belief that this region could be unstructured (Perkins, Lusy & John, 4837).
Two other regions were equally examined to check whether their muattions also had some effects on the stability of CDC6. Two CDC6 alleles were constructed using site directed mutagenesis where serine 372 was transformed to alanine (S372A) and in the other, serine 354 was changed alanine ...Download file to see next pagesRead More
Cite this document
  • APA
  • MLA
  • CHICAGO
(“Cell cycle by emphasizing DNA replication Research Paper”, n.d.)
Cell cycle by emphasizing DNA replication Research Paper. Retrieved from https://studentshare.org/biology/1621631-cell-cycle-by-emphasizing-dna-replication
(Cell Cycle by Emphasizing DNA Replication Research Paper)
Cell Cycle by Emphasizing DNA Replication Research Paper. https://studentshare.org/biology/1621631-cell-cycle-by-emphasizing-dna-replication.
“Cell Cycle by Emphasizing DNA Replication Research Paper”, n.d. https://studentshare.org/biology/1621631-cell-cycle-by-emphasizing-dna-replication.
  • Cited: 0 times
Comments (0)
Click to create a comment or rate a document
CHECK THESE SAMPLES - THEY ALSO FIT YOUR TOPIC
DNA Evidence
Historically, DNA evidence began in the forensic field when ABO blood group was used as a system in providing evidence of crime issues. Later, the paternity identification that based on serum protein variation became commonly used. However, it was not until when Jeffrey became a pioneer of DNA based identity testing.
3 Pages(750 words)Research Paper
Cell Cycle Progression: A Coordinated Act of Cyclins, Cdks and Checkpoints
The cell cycle consists of two major steps: Interphase and mitotic phase. The cell spends most of its time in the interphase. In this phase, the cell grows, doubles the contents and duplicates the DNA. The interphase consists of 4 sub phases. They are G1 phase, S phase, G2 phase and Mitotic phase.
5 Pages(1250 words)Research Paper
DNA Repair Mechanisms
Since different strains of the bacteria react differently to gene replacement with lacS gene, these researchers hypothesize that different S. solfataricus strains have different DNA processes as well, including their repair mechanisms. First, each strain was cultured using 0.2 to 0.4 in basal salts medium with 0.1% (wt/vol) sucrose and 0.1% (wt/vol) tryptone (ST medium) or 0.2% (wt/vol) glucose and 0.2% (wt/vol) tryptone (GT medium) prior to UV exposure.
4 Pages(1000 words)Research Paper
DNA Fingerprinting
Name Institution Course Instructor Date DNA Fingerprinting Abstract DNA fingerprinting is a laboratory procedure that has proved very useful in personal identification. With its wide applications, it is causing a revolution in the modern world. Typing of DNA presents unique profiles for each individual except identical twins.
5 Pages(1250 words)Research Paper
Optimizing the condition of the PCR detecting of PTEN gene in DNA derived from human cell lines, associated with ageing and senescence
Over the past several years, there has been an emerging aging research theme linking cellular processes influencing cancer with those influencing
15 Pages(3750 words)Research Paper
DNA fingerprinting
Every strand is composed of nucleotides, a sequence of bases. A base can be one of the four chemicals, adenine, thymine, cytosine, or guanine. The two DNA strands are connected to every base. Bases can only bond with each other as follows: Guanine (G) can only bond
3 Pages(750 words)Research Paper
The Cell Cycle
Mitosis, on the other hand, involves division of nuclear chromosomes. It has four phases namely the prophase, metaphase, anaphase, and telophase. In prophase, chromatin material is condensed into distinct chromosomes that migrate to the center of the cell. At the opposite poles of the cell, there is the formation of spindle fibers.
2 Pages(500 words)Essay
Mitosis & Cell Cycle: Cancer Treatments
Cancer can affect in any part of body tissues and organs. These include bones, skin, nerve tissue, breast, stomach, colon, retina, and lungs among other areas (Russo & Russo, 2004). This paper explores breast cancer. Breast cancer
1 Pages(250 words)Essay
Introduction about cell cycle by emphasizing DNA replication
DNA replication occurs in cells to ensure that the daughter cells contain genetic material from the parent cell through a process known as semi-conservative replication (Kurth & Gautier, 2010). DNA replication relies
2 Pages(500 words)Research Paper
A Study of Chromosome formation through observation of the cell cycle (Abstract)
The key to this process is observing the cell-cycle. Cell divisions occur in two parts: the interphase and the miotic phase, the former of which can be further sub-divided into the G1, S and G2 phase. We observed the somatic cell division and meiosis stages to get an understanding of the process of chromosomal formation.
2 Pages(500 words)Research Paper
sponsored ads
We use cookies to create the best experience for you. Keep on browsing if you are OK with that, or find out how to manage cookies.
Let us find you another Research Paper on topic Cell cycle by emphasizing DNA replication for FREE!
logo footer
Contact us:
+16312120006
Contact Us Now
FREE Mobile Apps:
  • StudentShare App Store
  • StudentShare Google play
  • About StudentShare
  • Testimonials
  • FAQ
  • Blog
  • Free Essays
  • New Essays
  • Essays
  • Miscellaneous
  • The Newest Essay Topics
  • Index samples by all dates
Join us:
Contact Us