StudentShare
Contact Us
Sign In / Sign Up for FREE
Search
Go to advanced search...
Free

How to make and run an agarose gel (DNA Electrophoresis) - Article Example

Cite this document
Summary
Abstract: For isolation of genomic DNA, agarose gel electrophoresis is done which is based on the principle that on disruption and lysis of cell components, followed by removal of proteins and sugar, nucleic acids can be recovered which can be observed through a UV…
Download full paper File format: .doc, available for editing
GRAB THE BEST PAPER98.3% of users find it useful
How to make and run an agarose gel (DNA Electrophoresis)
Read Text Preview

Extract of sample "How to make and run an agarose gel (DNA Electrophoresis)"

Agarose Gel Electrophoresis of DNA For isolation of genomic DNA, agarose gel electrophoresis is done which is based on the principle that on disruption and lysis of cell components, followed by removal of proteins and sugar, nucleic acids can be recovered which can be observed through a UV transilluminator on the agarose gel due to prior staining by ethidium bromide.Materials: Agarose powder, Electrophoresis buffer (TAE), gel casting tray, Ethidium bromide, Comb, Sample, Loading buffer, UV transilluminator.

Method: Agarose solution (generally .8%) is prepared for isolation of DNA. However, the concentration depends upon the sample i.e. the size of DNA. Appropriate amount of agarose powder is weighed and mixed with electrophoresis buffer. The two are mixed properly and heated till the solution is clear. The solution is then left to cool till it reaches 60C. Then 1µLof ethidium bromide is added to the solution and mixed properly. This helps in observing the sample against the UV transilluminator.

The solution is poured in a gel casting tray, the two open sides of which have been covered with a cellotape. Place a comb lightly at one end and the tray is left as such to allow the gel to set. When the gel is set the sample can be loaded in the wells. The sample is mixed with the loading buffer, approximately .2 volumes of it and loaded in the well. The first well can be loaded with a marker, which can be used to determine the size of the DNA sample. The gel is run at 50V for 45 min.

The gel is observed under UV transilluminator to check the sample.Result: http://www.methodbook.net/dna/agarogel.html

Read More
Cite this document
  • APA
  • MLA
  • CHICAGO
(“How to make and run an agarose gel (DNA Electrophoresis) Article”, n.d.)
How to make and run an agarose gel (DNA Electrophoresis) Article. Retrieved from https://studentshare.org/biology/1605894-how-to-make-and-run-an-agarose-gel-dna-electrophoresis
(How to Make and Run an Agarose Gel (DNA Electrophoresis) Article)
How to Make and Run an Agarose Gel (DNA Electrophoresis) Article. https://studentshare.org/biology/1605894-how-to-make-and-run-an-agarose-gel-dna-electrophoresis.
“How to Make and Run an Agarose Gel (DNA Electrophoresis) Article”, n.d. https://studentshare.org/biology/1605894-how-to-make-and-run-an-agarose-gel-dna-electrophoresis.
  • Cited: 0 times

CHECK THESE SAMPLES OF How to make and run an agarose gel (DNA Electrophoresis)

BIOTECHNOLOGY & GENETIC ANALYSIS PRACTICAL ASSESSMENT

) The second and confirmatory test is isolating the plasmid from white colonies and run on to the agarose gel electrophoresis.... (If using the photo below you should note that it was run under different electrophoresis conditions to those we used in the prac and the relative positions of supercoiled and linear plasmid may be different.... Explain exactly what is happening in steps 2,3,4 and 5 in the "Wizard" preparation (protocol 3), and how this begins the separation of plasmid dna from chromosomal dna....
2 Pages (500 words) Essay

Polymerase Chain Reaction, Fingerprinting for Crime Detection

Invention of Polymerase chain reaction (PCR) by Kery Mullis in 1983(1) has revolutionized the way dna was looked upon.... PCR as name suggests is an enzyme based in vitro technique by which one can generate millions of copy of given dna in short time.... hellip; The major constituents of PCR are, 1) dna polymerase 2) short dna fragment as primer 3) dNTPs 4) buffer and 5) dna template.... Invention of thermo stable dna polymerase from thermophilic bacterium Thermus aquaticus (Taq Pol) (2) has made PCR a routine process in all molecular biology laboratories....
5 Pages (1250 words) Essay

The Use of Gel Electrophoresis

The solidified agarose gel is inserted into the electrophoresis chamber and is just covered with buffer.... One purine pair with one Pyrimidine with hydrogen bond to make the double stranded DNA.... These DNA fragments are separated using gel electrophoresis.... The technique of electrophoresis is based on the fact that since DNA contains phosphate group, it is negatively charged at the neutral pH.... It is incorporated in the gel so that staining occurs during electrophoresis....
3 Pages (750 words) Lab Report

Conventional PCR using agarose gel electrophoresis detection

This binds strongly to DNA by intercalating between the bases and is fluorescent meaning that it Conventional PCR using agarose gel electrophoresis detection According to Lewis “agarose gel electrophoresis is the easiest and commonest way of separating and analyzing DNA”.... “agarose gel Electrophoresis (Basic Method)”.... ontinue loading the samples and finish of with a final lane of markerClose the gel tank, switch on the power-source and run the gel at 5V/cm....
2 Pages (500 words) Essay

Effect of Ocean acidification upon ability to genetically adapt in Nereis species

The aims of this project are to examine whether Nereid worms under low pH conditions show differences in the expression of genes and physiological metabolites (or proteins), and secondly how this environmental pressure might affect the animals ability to maintain functional… Therefore the project aims to determine if the worms obtained from natural CO2 vents are different from those living under controlled conditions, in terms of behaviour and physiology.... ,363), ragworm (Nereis) species utilises As such the project will examine how environmental stress manifest itself upon regulation of reproduction, and ultimately, reproductive success....
4 Pages (1000 words) Essay

Essential Questions For Viruses

The host cell nucleus is the site where viral dna duplicates and the capsid proteins are imported into nucleus for assembly.... You should be able to do this for dna viruses and the four types of RNA viruses described in the lecture.... d) Biosynthesis: dna viruses start with transcription whereas RNA viruses proceed to translation if it is a + plus strand.... Viral dna is replicated in the host nucleus and capsid proteins are imported into the nucleus for assembly....
16 Pages (4000 words) Assignment

Clinical Microbiology & Molecular Biology Practices

In the paper “Clinical Microbiology & Molecular Biology Practices” the author discusses the agarose gel electrophoresis method, which depends on the agarose concentration, electrophoresis conditions, and the quality of the gel preparation to separate distinct fragments.... he agarose gel electrophoresis method depends on the agarose concentration, electrophoresis conditions, and the quality of the gel preparation to separate distinct fragments....
7 Pages (1750 words) Assignment

Agarose Gel Electrophoresis of DNA

Since DNAs mass/charge ratio is uniform, its molecules within an agarose gel are separated by size in a certain pattern such that the traveled distance is inversely proportional to its molecular weight log.... Gel electrophoresis refers to the method… Gel electrophoresis is applied in clinical chemistry to separate proteins by size and/or charge and in molecular biology and biochemistry to The paper "agarose gel Electrophoresis of DNA" is an outstanding example of an essay on biology....
3 Pages (750 words) Term Paper
sponsored ads
We use cookies to create the best experience for you. Keep on browsing if you are OK with that, or find out how to manage cookies.
Contact Us