quora

How to make and run an agarose gel (DNA Electrophoresis) - Article Example

Comments (0) Cite this document
Summary
Abstract: For isolation of genomic DNA, agarose gel electrophoresis is done which is based on the principle that on disruption and lysis of cell components, followed by removal of proteins and sugar, nucleic acids can be recovered which can be observed through a UV…
Download full paperFile format: .doc, available for editing
GRAB THE BEST PAPER97.6% of users find it useful
How to make and run an agarose gel (DNA Electrophoresis)
Read TextPreview

Extract of sample
"How to make and run an agarose gel (DNA Electrophoresis)"

Download file to see previous pages The two are mixed properly and heated till the solution is clear. The solution is then left to cool till it reaches 60C. Then 1µLof ethidium bromide is added to the solution and mixed properly. This helps in observing the sample against the UV transilluminator. The solution is poured in a gel casting tray, the two open sides of which have been covered with a cellotape. Place a comb lightly at one end and the tray is left as such to allow the gel to set. When the gel is set the sample can be loaded in the wells. The sample is mixed with the loading buffer, approximately .2 volumes of it and loaded in the well. The first well can be loaded with a marker, which can be used to determine the size of the DNA sample. The gel is run at 50V for 45 min. The gel is observed under UV transilluminator to check the ...Download file to see next pagesRead More
Cite this document
  • APA
  • MLA
  • CHICAGO
(“How to make and run an agarose gel (DNA Electrophoresis) Article”, n.d.)
How to make and run an agarose gel (DNA Electrophoresis) Article. Retrieved from https://studentshare.org/biology/1605894-how-to-make-and-run-an-agarose-gel-dna-electrophoresis
(How to Make and Run an Agarose Gel (DNA Electrophoresis) Article)
How to Make and Run an Agarose Gel (DNA Electrophoresis) Article. https://studentshare.org/biology/1605894-how-to-make-and-run-an-agarose-gel-dna-electrophoresis.
“How to Make and Run an Agarose Gel (DNA Electrophoresis) Article”, n.d. https://studentshare.org/biology/1605894-how-to-make-and-run-an-agarose-gel-dna-electrophoresis.
  • Cited: 0 times
Comments (0)
Click to create a comment or rate a document
CHECK THESE SAMPLES - THEY ALSO FIT YOUR TOPIC
Budget business PCs that run Vista
Nevertheless, still there are a number of high performances, inexpensive business PCs that come with both Windows Vista and Windows XP options. These are Dell OptiPlex740, the HP Compaq dc 5750 and the Lenovo 3000J115.
2 Pages(500 words)Article
How to make make money online
For gaining insight and understanding, it is significant that an individual finds free website tutorials as this helps the work flow and the design process of the website. After the website tutorials have been understood in a proper manner and the details are comprehended easily, the next step is to publish your webpage (Ploster 2004).
1 Pages(250 words)Article
How Electronic Medical Record could help to make decisions for patient
The electronic medical record (EMR) is a computerized system of storing medical records used by an individual healthcare provider such as a hospital or a physician (Carter, 2001). It is usually unique and is optimized to serve that particular facility or institution.
11 Pages(2500 words)Article
Review paper for optical-Ct gel-dosimetry
These traditional systems of delivery were restricted to single point delivery and 2-Dimensional plane deliveries which are not adequate for the highly complex contemporary requirements of delivery. In an effort to curb this menace, work has been done on a new system which has highly promising results.
8 Pages(2000 words)Article
Bits of Mystery DNA
These discoveries have shed light on how these discoveries can actually result into better health outcomes. This article discusses about the structure of DNA, earlier discoveries which were termed as Junk has now suddenly become important in
3 Pages(750 words)Article
One article analyses and make the Recommend marketing strategies to the relevant cloths industry on your analysis of the chosen phenomenon
A whopping 85 percent of the 25,000 respondents interviewed in this survey, which rated 39 online clothiers, expressed their satisfaction with their online shopping experience for clothes. L.L.Bean, Zappos, and
2 Pages(500 words)Article
How Will We Love
In this sense, marriage is merely borne out of romantic relationships. On the other hand, the older people seem to know that marriage demands the readiness for commitment. In a huge sense, young people hold an unrealistic
1 Pages(250 words)Article
'Loser Pays' Rules Make A Comeback
However, this process has led to the emergence of multiple lawsuits in a small frame of time, pushing the authorities to question if such lawsuits should be allowed to proceed. In light of this matter, authorities have reverted to applying a loser-pays rule which will
4 Pages(1000 words)Article
What do other people think about your personality traits (what impression do you give to other people) How do you know you make this particular impression on people
Their views regarding my personality characteristics vary in accordance to how close I am to them and how I treat them. Those who are close to me may not identify me as an introvert or a person who keeps to himself but they are well aware
1 Pages(250 words)Article
How sociology affects my life
As stated by Alex and Thio, this is sociological imagination, a term “for the ability of being able to see the effect of social forces on persons particularly on their
1 Pages(250 words)Article
sponsored ads
We use cookies to create the best experience for you. Keep on browsing if you are OK with that, or find out how to manage cookies.
Let us find you another Article on topic How to make and run an agarose gel (DNA Electrophoresis) for FREE!
logo footer
Contact us:
+16312120006
Contact Us Now
FREE Mobile Apps:
  • StudentShare App Store
  • StudentShare Google play
  • About StudentShare
  • Testimonials
  • FAQ
  • Blog
  • Free Essays
  • New Essays
  • Essays
  • Miscellaneous
  • The Newest Essay Topics
  • Index samples by all dates
Join us:
Contact Us