Culture dependent vs culture independent methods - Lab Report Example

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Outline the different bacterial species in the oral cavity as detected by 16S rRNA sequencing and culture-dependent traditional microbiology. Are there any similarities amongst the groups of bacteria detected by one identification method and not the other?
Some of the…
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Culture dependent vs culture independent methods
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Some of the techniques that can be applied include, but not limited to performing rDNA PCR amplification on clinical specimens regarded sterile, such as blood. It is however advisable that this technique should not be employed with specimens originating from nonsterile sites such as faeces (Litton, 2010 p. 56). Collection of specimens such as conventional assays needs aseptic precautions.
Litton (2010, p.57) claims that in order to curb contamination DNA brought by specimen collection vials, ensuring that the environment where work goes on is well organised also helps with this. Contamination linked to personnel working in the laboratory can be avoided by wearing cloves made of gloves or latex plus white coats. As for those contaminations resulting from consumable reagents and plastic wares, prior screening of each and every reagent before use in diagnostic assays (Litton, 2010 p. 56).
3. Find at least one peer-reviewed scientific research article regarding the bacteria that normally reside in the human mouth and provide references. Describe the groups of bacteria these studies identified to be present in the human mouth.
Some of the bacteria that reside in the mouth include but not limited to, staphylococcus with the most common ones being S.epidermidis and S.aureus. They are oval in shape and posses a thick cell wall, named gram-positive. They cause infections in human population when presented with optimal conditions (“New bacterial species found in human mouth” 2008, p.26).
Bacteria from the genus streptococcus forms the largest number of all the organisms found in the mouth. Some of the species here include, but not restricted to, S. mutans, S. mitis, S. salivarius, S. pneumoniae and S. Pyogenes. They are also oval in shape like the staphylococcus. S.mutans is also responsible for cavity formation in teeth by converting sucrose sugar into lactic acid which ...Download file to see next pagesRead More
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