Nobody downloaded yet

Nucleic acid hybridisation and Nucleic acid probes - Essay Example

Comments (0) Cite this document
The unique property of nucleic acids to pair with each other through complementary base pairing is the lifeblood of genetic engineering. A single strand of DNA can pair up with another strand of DNA or RNA if its base pairs are…
Download full paperFile format: .doc, available for editing
GRAB THE BEST PAPER98.7% of users find it useful
Nucleic acid hybridisation and Nucleic acid probes
Read TextPreview

Extract of sample
"Nucleic acid hybridisation and Nucleic acid probes"

Download file to see previous pages For instance, if a DNA strand with a desired nucleotide sequence is to be detected from a mixture of many other strands, an oligonucleotide containing a few complementary bases to the desired sequence can be prepared and attached to an anchor such as a membrane or a paper. When soaked in a solution having a mixture of many strands, the one, which is complementary to the oligonucleotide, will bind to it through complementary base pairing, also known as “zippering” (Lodish et al, 2004, p. 11).
When double stranded DNA is heated in a dilute salt solution, its two strands separate because of the breakdown of complementary base pairing (melting). This strand separation is called denaturation. The temperature at which the two complementary strands separate is called the melting temperature ‘Tm’, and is affected by the percentage of G.C base pairs, ion concentration of the solution, presence of destabilising compounds like urea, and the pH of the solution (Lodish et al, 2004, p. 105).
A particular fragment of DNA or RNA whose nucleotide sequence is complementary to a gene or nucleotide of interest is called a nucleic acid probe. A nucleic acid probe has to be designed in such a way that it hybridises through complementary base pairing to the target DNA or RNA that has to be detected. It should be long enough (about 20 nucleotide long) to pair exclusively to the target nucleotide sequence. Probes are labeled with radioactive tracers, histochemical compounds or fluorescent dyes to enable their detection from a heterogeneous mixture of nucleic acids (Nussbaum et al, 2004, p.41). For instance, 32P labeled probes are developed using polynucleotide kinase that transfers a ...Download file to see next pagesRead More
Cite this document
  • APA
  • MLA
(“Nucleic acid hybridisation and Nucleic acid probes Essay”, n.d.)
Retrieved from https://studentshare.org/biology/1578618-nucleic-acid-hybridisation-and-nucleic-acid-probes
(Nucleic Acid Hybridisation and Nucleic Acid Probes Essay)
“Nucleic Acid Hybridisation and Nucleic Acid Probes Essay”, n.d. https://studentshare.org/biology/1578618-nucleic-acid-hybridisation-and-nucleic-acid-probes.
  • Cited: 0 times
Comments (0)
Click to create a comment or rate a document
Acid mine drainage
Table of contents Abstract: This paper sets out to examine the causes of acid mine drainage of a site under investigation-a long-abandoned coal mine. The analyses of the samples collected were done to ascertain the alleged source of the river contamination.
4 Pages(1000 words)Essay
Tranexamic Acid
This synthetic antifibrinolytic agent is derived from amino acid lysine. This paper examines various print and online literatures that provide information on the use of tranexamic acid. Literature on the use of tranexamic acid in orthopedic surgery and other Hemorrhagic conditions Guatam, Katyal, Yamin, and Singh argue that tranexamic acid greatly decreases bleeding after operation and reduces the need for blood transfusion among patients after undergoing a total knee replacement (TKR) (590).
8 Pages(2000 words)Essay
The Acid Base Disturbance
The Acid Base Disturbance in this patient is caused by a condition known as metabolic alkalosis. Consequently, this state occurs when the PH of the blood in arteries rise above 7.45 which is the maximum PH. The pH above 7.5 is termed as alkalemic. A general decline in hydrogen ion concentration or a surplus of base leads to alkalosis.
2 Pages(500 words)Essay
Acid rain
Acid rain can be prevented through a number of ways in future. According to Casper, acid rain can be prevented in future is by switching to sources of energy that emit low levels of carbon.3 Combustion of
1 Pages(250 words)Assignment
Aristolochic Acid
Urinary tract damage and other cancers have been linked to the intake of aristolochic acid as well as kidney damage and kidney failure which requires dialysis or worse, kidney transplantation [2]. Therefore, this makes aristolochic acid both nephrotoxic and carcinogenic [11].
11 Pages(2750 words)Essay
Composition of acid rain
"The pH scale is used to measure the acidity or alkalinity of an aqueous solution and is determined by the hydrogen ion (H+) content" ("Measuring Acid Rain"). This scale ranges from 0 to 14, moving from strongly acid to strongly alkaline, respectively, with the point 7 representing neutral.
10 Pages(2500 words)Essay
Acid mine drainage
A comparison between the analysed levels of the pH of the site to determine the source of contamination based on the Acid generating Potential and the Acid Neutralizing Capacity (ANC) which is calculated directly by adding acid to a slurry of the spoil
4 Pages(1000 words)Essay
Acid Ratios
There are many ratios that are used to determine a ratio analysis. These are short-term solvency ratios, debt management ratios, asset management ratios, profitability ratios, and market value ratios. The two ratios that make
4 Pages(1000 words)Essay
Acetic acid from methanol
Cativa system has been used largely to produce acetic acid from methanol through a number of processes that we shall discuss for the production of 250,000 t/year of acetic acid. Methanol also
4 Pages(1000 words)Essay
Acid Rain Part I
In response to the causative agent of the acidic rain, it is lobbied that Pennsylvania has many industries which emit poisonous pollutants into the atmosphere (Park, 2013). These pollutants
1 Pages(250 words)Essay
sponsored ads
We use cookies to create the best experience for you. Keep on browsing if you are OK with that, or find out how to manage cookies.
Let us find you another Essay on topic Nucleic acid hybridisation and Nucleic acid probes for FREE!
logo footer
Contact us:
Contact Us Now
FREE Mobile Apps:
  • StudentShare App Store
  • StudentShare Google play
  • About StudentShare
  • Testimonials
  • FAQ
  • Blog
  • Free Essays
  • New Essays
  • Essays
  • Miscellaneous
  • The Newest Essay Topics
  • Index samples by all dates
Join us:
Contact Us