Molecular Biology - Lab Report Example

Comments (0) Cite this document
The promoter regions are shown in blue. The RNA polymerase III promoter structure refers to the 5S rRNA genes. It is evident that other genes transcribed by RNA polymerase III have different promoter structures, including some with a TATA box and upstream elements similar to an RNA polymerase II promoter…
Download full paperFile format: .doc, available for editing
GRAB THE BEST PAPER97.9% of users find it useful
Molecular Biology
Read TextPreview

Extract of sample
"Molecular Biology"

Download file to see previous pages Dpn I and Fse I together: fragments of 0.5 kb, 1.1 kb, 1.6 kb and 2.3 kb
Dpn I, Eag I and Fse I together: fragments of 0.3 kb, 0.5 kb, 0.6 kb, 1.0 kb, 1.1 kb and 2.0
a) How many restriction sites are there for each enzyme What, if any, are the unique restriction sites on this plasmid
Ans. Dpn I = 3, Eag I = 2, Fse I = No RS. There are unique restriction sites for Fse I, this restriction enzyme works in conjunction with the Dpn I and Eag I.
b) Construct a restriction map of the plasmid and draw it below.

Cloning Strategies
Question 4 (28%)
Describe outline cloning strategies, including vector types (individual vectors need not be specified) and methods used at each stage, for the following scenarios:
Worked example
You wish to isolate the coding sequence of a human liver enzyme. You have purified the corresponding bovine enzyme and have raised a polyclonal antibody against it.
- Make a cDNA library from human liver tissue - this will be enriched for the
genes for liver enzymes.
- Create the library in an expression vector with a strong promoter so the genes
are expressed in the host.
- Screen the induced expression library for the presence of the desired liver
enzyme using the bovine polyclonal antibody. The antibody will bind to the
colonies which produce the protein they recognise. Although the match may
not be exact there should be enough conserved homology to ensure
- Positive colonies will be identified by visualising the label on the bound
antibody/secondary antibody in the colony hybridisation.
a) You have a cDNA clone containing the 900 bp coding sequence of a cell surface
protein from pygmy goat monocytes. How can you use this to find the
homologous cDNA from the merino sheep
b) Having...
The results are as follows:
step. f1 IG SEQUENCE: to make single stranded DNA for sequencing UNIVERSAL PRIMER SEQUENCE: for primer to anneal to, to initiate sequencing SELECTABLE MARKER (eg lacZ'): to allow selection of clones containing the insert MCS POLYLINKER: insert fragment of DNA here 3.0 kb
You must describe the function of the essential features of each plasmid and give some indication of the plasmid size. For expression vectors you must bear in mind the host cells in which the coding sequence will be expressed.
a) Nonsense: The nonsense-mediated mRNA decay pathway degrades mRNAs transcribed from genes in which an amino-acid codon has changed to a nonsense codon; this prevents the translation of such mRNAs into truncated, and potentially harmful, proteins.
c) Splicing: A stage in the processing of mRNA, occurring only in eukaryotic cells, in which intervening sequences (introns) are removed from the primary RNA transcript (hnRNA) and the codig exons are joined together to form the mature mRNA molecule. url:www.geneontology.org .
d) Promoter: A nucleotide sequence of DNA to which RNA polymerase binds and initiates transcription. It usually lies upstream of (5' to) a coding sequence. A promoter sequence aligns the RNA polymerase so that transcription will initiate at a specific site.
e) Reading Frame: A series of triple ...Download file to see next pagesRead More
Cite this document
  • APA
  • MLA
(“Molecular Biology Lab Report Example | Topics and Well Written Essays - 1750 words”, n.d.)
Retrieved from https://studentshare.org/biology/1531386-molecular-biology
(Molecular Biology Lab Report Example | Topics and Well Written Essays - 1750 Words)
“Molecular Biology Lab Report Example | Topics and Well Written Essays - 1750 Words”, n.d. https://studentshare.org/biology/1531386-molecular-biology.
  • Cited: 0 times
Comments (0)
Click to create a comment or rate a document
Molecular Basis of Disease
Cancer are abnormal human cells characterized by “unregulated growth, impaired cellular differentiation, invasiveness and metastatic potential” (Coleman, The Molecular Basis of Human Cancer, 2002, p.3). These characteristics of cancer cells have been used to clinically distinguish cancer cells from normal cells during diagnosis and treatment.
8 Pages(2000 words)Lab Report
Protein Quantitation
Bradford assays are among the methods used to determine the concentration of protein, relative to a standard. This technique is based on the formation of a complex between the dye, Brilliant Blue G and proteins in solution. The complex being formed causes a shift in the absorption peak from 465nm to 595nm.
5 Pages(1250 words)Lab Report
Molecular Basis of Pernicious Anaemia
Others include infiltration of inflammatory cells into the stomach and production of auto- antibodies to various proteins. It also involves the intrinsic factor and proton pump of parietal cells whereby intrinsic factor can be identified as the factor that is involved in the absorption of vitamin B12.
6 Pages(1500 words)Lab Report
Biochemistry Lab Report
To confirm the success of transformation, the recombinant DNA was isolated and purified from the transformants followed by digestion using HIND III /EcoRV. The GFP protein was then purified from the transformants using hydrophobic interaction chromatography (HIC) and its purity confirmed using immunoblotting and SDS-Page.
3 Pages(750 words)Lab Report
DNA Practical
The DNA from mitochondria and chloroplast is responsible for cytoplasmic inheritance or maternal inheritance. DNA is a double stranded nucleic acid and both the strands run antiparallel. It is made up of pentose sugar (de-oxy ribose), phosphate group and nitrogen base pairs (Double ringed Purines: Adenine and Guanine and Single ringed Pyrimidine: Cytosine and Thymine).
4 Pages(1000 words)Lab Report
Lab report on NITROGEN
itions caused the creation of organic molecules containing the principle elements: carbon, oxygen, sulfur, phosphorus, fluorine, bromine, chlorine, and nitrogen. In particular, nitrogen is important to biological functioning and may have something to do with the fact that
2 Pages(500 words)Lab Report
Cell biology
A microscope has very many parts that play major roles in the magnification of specimen. These parts are build together to ensure that they work together to achieve magnification. The microscope has two systems of lenses which are known as the ocular and the objective lens
10 Pages(2500 words)Lab Report
Determination of Protein using Biuret Reagent
In case the bond is detected, copper (II) ions turn violet in an alkaline solution. In performing this test, several variants can be considered. Despite the name, the chemical does not contain burette ((H2N-CO-)2NH). This reaction is named using that name because it
3 Pages(750 words)Lab Report
Molecular Genetics Practical Write Up
Salmonella typhimurium is a leading cause of gastroenteritis in humans This experiment tested the histidine requirements of the auxotrophic mutant strains and their spontaneous reversion rate. The experiments were carried using either wild
7 Pages(1750 words)Lab Report
Molecular Biology
Monoclonal antibodies can be obtained by intraperitoneal injection into mice and collection of ascetic fluid produced. Since it is difficult to to remove antigenicity from the mouse, in vitro
8 Pages(2000 words)Essay
sponsored ads
We use cookies to create the best experience for you. Keep on browsing if you are OK with that, or find out how to manage cookies.
Let us find you another Lab Report on topic Molecular Biology for FREE!
logo footer
Contact us:
Contact Us Now
FREE Mobile Apps:
  • StudentShare App Store
  • StudentShare Google play
  • About StudentShare
  • Testimonials
  • FAQ
  • Blog
  • Free Essays
  • New Essays
  • Essays
  • Miscellaneous
  • The Newest Essay Topics
  • Index samples by all dates
Join us:
Contact Us