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Antibodies - Essay Example

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Discovery and Development of 1,2-Propanediol (Propylene Glycol) Introduction Innumerable products of industrial microbiology have been developed and produced through various biotechnological approaches such as metabolic pathway engineering and metabolic control engineering of microorganisms…
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Download file to see previous pages It is used in the manufacture of unsaturated polyester resins and is used as solvent for surfactants, paints and cleaning agents1. It is also used as antifreeze for de-icing aircrafts at airports as it lowers the freezing point of water. Recent applications include use as a humectant and a preservative. It is used in cosmetics, pharmaceutical preparations and also in electronic cigars2. The structure of 1,2-Propanediol comprises of three carbons with a stereogenic center at the central carbon3. It exists in two racemic forms: S and R as shown in figure 1 below. Figure 1: Chemical structure (S and R form) of 1,2-propanediol 3 1,2-Propanediol as well as 1,3-Propanediol can be manufactured though bioengineered microorganisms. By turning on and off some genes, it is possible to make the microorganism overproduce a specific metabolic product. As shown in figure 2, the intermediate metabolism of Escherichia coli can be shifted to produce 1,2 and 1,3-propanediol using enzymes such as aldose reductase from rat lens, E. coli glycerol dehydrogenase and Klebsiella pneumoniae glycerol dehydrogenase and 1,3-propanedioloxidoreductase4. There are various biochemical pathways through which 1,2-propanediol can be produced. Figure 2: Metabolic pathway engineering of E. ...
It was shown that E. coli can grow on the L-fucose and L-rhamnose deoxy sugars as sole carbon and energy sources resulting in the production of propanediol. The first instance of metabolic engineering to produce 1,2-propanediol was when Altaras and Cameron metabolically bioengineered the 1,2-propanediol pathway in E. coli7. In 1999, they reported that E. coli that overexpressed methylglyoxal synthase gene produced 1,2-propanediol. Expression of methylglyoxal synthase or glycerol dehydrogenase led to anaerobic production of around 0.25 g of 1,2-propanediol per liter. The yield was found to be higher both the enzymes were coexpressed7. The development After their first report of the production of 1,2-propanediol from metabolically engineering E. coli, Altaras and Cameron again in 2000 published another report. This time they reported enhanced production of 1,2-propanediol8. They investigated three methods. First method involved the elimination of lactate byproduct. The second method involved the construction of a complete pathway from dihydroxyacetone phosphate – an intermediate of the glycolytic cycle. The third method involved bioprocessing improvements through fed-batch fermentation using the best bioengineered strains. They were able to successfully produced a final yield of 0.19 g of 1,2-propanediol per gram of consumed glucose. Many later studies have investigated the production of 1,2-propanediol by metabolically engineered bacteria. Berrios-Rivera, San and Bennett studied the effects of various cofactor manipulations on the production of 1,2-propanediol9. They used sugars that were similar to glucose and that can be fed into glycolysis for pyruvate production. The sugars used had different oxidation ...Download file to see next pagesRead More
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