quora

DETECTION OF ANTIVIRAL ANTIBODIES IN SERUM USING AN ELISA TECHNIQUE - Essay Example

Comments (1) Cite this document
Summary
Detection of antiviral Antibodies in Serum using an ELISA technique Introduction Enzyme linked Immuno Sorbant Assay (ELISA) is the most commonly used immunological method for the identification of many diseases. It is also called as enzyme assay. ELISA is used to detect the antigens present in the given sample by using the specific antibodies…
Download full paperFile format: .doc, available for editing
GRAB THE BEST PAPER94.3% of users find it useful
DETECTION OF ANTIVIRAL ANTIBODIES IN SERUM USING AN ELISA TECHNIQUE
Read TextPreview

Extract of sample
"DETECTION OF ANTIVIRAL ANTIBODIES IN SERUM USING AN ELISA TECHNIQUE"

Download file to see previous pages These immunoglobulins are of five types and they are different based on size, charge, amino acid composition and carbohydrate content. the class and the type of immunoglobulin molecule is determined by the heavy chain type. There are two light chains and two heavy chains. There are C and N- terminal in the heavy chains. (Crowther 1995). The N-terminals of the IgG constitute the antigen -binding site. Individuals can become resistant to many infectious diseases by active or passive immunization. The beneficial effects of the immunization are mediated by the antibodies and the effect of immunization is mediated by the immunoassays. (Crowther 2001). The measurement of the antigen and antibody by using sensitive assays are the useful indicators of the immune status. When an infectious agent enters the human body, the component is identified as the foreign particle and antibodies are produced against them. These antibodies interact with the antigens and reduce the load. (Edwards 1999). The immunoassay method identifies the antigens, antigen - particles present in the blood and provide the results. Each antibody is specific for an antigen. ELISA is basically of two types: competitive and non-competitive ELISA. ELISA involves two site recognition with two different antibodies (i.e.) an indirect estimation. Competitive ELISA is different from the sandwich ELISA and indirect ELISA. In the first step the unlabelled antibody is incubated on the presence of the antigen. This antigen-antibody bounded is added to the antigen- coated well. The plate is washed and the second antibody specific to the primary antibody is added and the second antibody is coupled to the enzyme. When the substrate is added, the signal is emitted by the remaining enzyme. In this ELISA, if the antigen concentration is very high, then the signal will be very weak. This method is mainly used to test the impure and crude samples. This method is very useful for the detection of HIV. For HIV diagnosis, the patient's sample and the enzyme-labeled antibody (conjugate) is added simultaneously to the solid phase. HIV antibodies, if present in the sample will bind to the conjugate antibody and reduce the signal. Thus if the color reaction is produced, then it indicates that there are no HIV antibodies in the given sample. Applications of ELISA include screening of the donated blood for viral contaminations( HIV types, Hepatitis B and C, HTLV-1 and 2), measuring the hormone levels( pregnancy(HCG), ovulation( LH), thyroid function ( TSH, T3 and T4), anabolic steroids, hormones), detecting infections( HIV, syphilis, chlamydia, gondii), detecting allergens in foods and toxins in foods, measuring the concentration of the antibodies and detecting illicit drugs ( cocaine, opiates, marijuana, etc.), measuring auto antibodies in autoimmune diseases and measuring rheumatoid factors . (Sheehan 1997). (Wreghitt and Morgan-Capner 1990). Principle When an antibody is linked to the enzyme and allowed to react with the immobilized antigen, antigen-antibody reaction occurs. This is identified by the production of colour by the substrate enzyme reaction. The antigen is immobilised in either a microtiter plate or on nitrocellulose membrane. An enzyme labelled antibody is added in excess to the system and these binds to the immobilised antigen. Excess antibody is removed by washing and the substrate specific for the ...Download file to see next pagesRead More
Cite this document
  • APA
  • MLA
  • CHICAGO
(“DETECTION OF ANTIVIRAL ANTIBODIES IN SERUM USING AN ELISA TECHNIQUE Essay”, n.d.)
DETECTION OF ANTIVIRAL ANTIBODIES IN SERUM USING AN ELISA TECHNIQUE Essay. Retrieved from https://studentshare.org/biology/1454101-detection-of-antiviral-antibodies-in-serum-using
(DETECTION OF ANTIVIRAL ANTIBODIES IN SERUM USING AN ELISA TECHNIQUE Essay)
DETECTION OF ANTIVIRAL ANTIBODIES IN SERUM USING AN ELISA TECHNIQUE Essay. https://studentshare.org/biology/1454101-detection-of-antiviral-antibodies-in-serum-using.
“DETECTION OF ANTIVIRAL ANTIBODIES IN SERUM USING AN ELISA TECHNIQUE Essay”, n.d. https://studentshare.org/biology/1454101-detection-of-antiviral-antibodies-in-serum-using.
  • Cited: 0 times
Comments (1)
Click to create a comment or rate a document
la
ladarius97 added comment 1 month ago
Student rated this paper as
Wonderful paper! Used it to finish an assignment for a biology course. It was easy as ABC, for the first time in my life.
CHECK THESE SAMPLES - THEY ALSO FIT YOUR TOPIC
Stock Markets
The efficient capital market hypothesis as applied to stock markets has affected the thinking of investment managers, corporate finance officers, and investors in the last three decades. This paper explains what the hypothesis is about based on a chapter in the textbook written by Elton, Gruber, Brown, and Goetzmann, and provides a critical assessment of its implications for the investor.
6 Pages(1500 words)Essay
Antibodies
One such product is 1,2-propanediol (propylene glycol), a chemical feedstock produced using the metabolic pathway engineering approach. 1,2-Propanediol is a colorless and clear liquid that has a high boiling point1. It is very hygroscopic in pure form. Industrially, it is a very useful product.
6 Pages(1500 words)Essay
INFECTION & IMMUNITY PRACTICAL : DETECTION OF ANTIVIRAL ANTIBODIES IN SERUM USING AN ELISA TECHNIQUE
Innate immunity is the immunity that occurs right from birth. Acquired or adaptive immunity is the type which is obtained based on the induction with response to foreign substances (Edwards 1999). The foreign substance called the antigen enters into the living body and the body recognizes it and evokes an immune response.
5 Pages(1250 words)Essay
The principles of ELISA and its application
It is often used as a diagnostic test to detect autoimmune disorders, cancer related diseases, detection of animal and plant pathogens, detection of pregnancy, human viral infections like hepatitis B Virus, Hepatitis C virus, etc (Business
4 Pages(1000 words)Essay
Conventional PCR using agarose gel electrophoresis detection
The reason to add gel in this method is to either quantify the DNA or separate the particular band of DNA. With the addition of edhidium bromide, DNA becomes visible. This binds strongly to DNA by intercalating between the bases and is fluorescent meaning that it
2 Pages(500 words)Essay
Antiviral Drugs
While not minimizing its achievements, the IOM report notes that PEPFAR imposes limitations upon its own potential through faulty budget and resource allocation framework, on the one hand, and its failure to allow local agencies and partners, comparative and constructive autonomy, on the other.At present, PREPFAR allocates a mere 20% to prevention and, in further constraint upon the ability and capacity of local agencies to combat the spread of AIDS, precisely articulates the prevention strategies which have to be implemented. Accordingly, it allocates a third of the prevention budget to abstinence programs, even though the latter may not be relevant within the cultural and societal environments of some of the PEPFAR target countries.
1 Pages(250 words)Essay
Clinical immunology (ELISA TYPES)
This is a fast method since there are no secondary antibodies added. However, other signal amplifications can be missed and these are often obtained through using secondary antibodies. The
3 Pages(750 words)Essay
Techniques for the Biomedical Sciences
ELISA was developed in 1970 as a replacement for radioimmunoassay. ELISA is a biochemistry assay that is wet in nature used in the detection of substance usually an antigen. The plates are washed in the first place to sterilize any microorganism that may be present that may alter the results of the experiment.
4 Pages(1000 words)Essay
Immunosorbent assay (ELISA)
Consequently, ELISA distinguishes itself from other forms of antibody-based assays, as there is a separation of specific and non-specific relations through sequential bidding to a solid phase; thus, making it possible for the achievement of the
7 Pages(1750 words)Essay
C-reactive protein as a novel biomarker
In this article, the authors review the role of CRP in the diagnosis, monitoring, and treatment of various forms of ischemic and inflammatory cardiovascular disease. Atherosclerosis is an inflammatory vascular disease characterized by endothelial activation, cellular influx, and production of mediators and cytokines.
8 Pages(2000 words)Essay
sponsored ads
We use cookies to create the best experience for you. Keep on browsing if you are OK with that, or find out how to manage cookies.
Let us find you another Essay on topic DETECTION OF ANTIVIRAL ANTIBODIES IN SERUM USING AN ELISA TECHNIQUE for FREE!
logo footer
Contact us:
+16312120006
Contact Us Now
FREE Mobile Apps:
  • StudentShare App Store
  • StudentShare Google play
  • About StudentShare
  • Testimonials
  • FAQ
  • Blog
  • Free Essays
  • New Essays
  • Essays
  • Miscellaneous
  • The Newest Essay Topics
  • Index samples by all dates
Join us:
Contact Us