Nobody downloaded yet

Monitoring Synaptic Function at the Neuromuscular Junction of a MouseExpressing SynaptopHluorin - Essay Example

Comments (0) Cite this document
Monitoring synaptic function at the neuromuscular junction of a Mouse expressing SynaptopHluorin Overview of Research Paper Presynaptic functions have greatly been influenced by optical measurements of exocytosis and endocytosis in nerve terminals (Lucia et al., 2007, p…
Download full paperFile format: .doc, available for editing
GRAB THE BEST PAPER93.5% of users find it useful
Monitoring Synaptic Function at the Neuromuscular Junction of a MouseExpressing SynaptopHluorin
Read TextPreview

Extract of sample
"Monitoring Synaptic Function at the Neuromuscular Junction of a MouseExpressing SynaptopHluorin"

Download file to see previous pages 5422). Selection of the neuromuscular junction of a mouse however came with a number of challenges for the researchers and this generally affected the tone of the research as the researchers were forced to “created a transgenic mouse (Bozza et al., 2004; Li et al., 2005; Wyatt and Balice-Gordon, 2005, 2006) that expresses spH at the NMJ” (Lucia et al., 2007, p. 5422). The researchers therefore based their work on the characterization of a number of hidden and unexpected properties of the phenotype of the synapse. Techniques used Four major techniques for data collection into the proof of the hypothesis set were used. These were vector construction, acute experimental conditions, generation transgenic of mouse and image analysis. Each of these techniques was used at different levels and for different purposes. This means that the techniques were not used simultaneously. With the vector constructions for instance, the transgenic vector known to be pThy1-SynaptopHluorin was obtained. The researchers used a method that had earlier been used by Ng et al., (2002), which subcloned a 1 kb EcoRI-SmaI fragment from pCiNeo-VAMP-superecliptic. This technique and the generation transgenic of mouse that followed it were important because it set the pace for the researchers to have an ideal clone that would perfectly fit the place of the transgenic mouse they created in terms of characteristics. Under the acute experimental conditions, the researchers made an attempt towards the actual goal by getting to the nerve-muscle of the mouse whereby they dissected the levator auris nerve–muscle (Lucas et al., 2007, p. 5424). The dissection was done based on the work of Katz et al., (1996). This particular technique was highly necessary in setting the pace for stimulus trains, which were delivered at 100 Hz. This was the work that had been previously done in conjunction with Bernard Katz. Through the work, they argued that ions became neutrally charged when combined with a lipid soluble carrier. This paradigm also predicted that any current that is incoming, has to be preceded by a flow of lipid carriers, which have to be outgoing. They therefore concluded that the justification of this is that the ions were being attracted through a charge-biased selective pathway, which, to overcome the membrane potential, had to perform work (Lucia et al. 5422). Data-base review of key findings The data collected from the experiment gave very new but equally important information on the neuromuscular junction of the mouse. This new information had to do with the fact that there is large size of the nerve terminals of the mouse that generally makes it possible to undertake spatial properties of exocytosis. A number of existing researches and findings were also confirmed in this research by Lucas et al (2007). For instance studies by Hodgkin-Huxley, which had earlier been conducted on the neurology of membrane current is largely confirms in the current research. Scientifically, the Hodgkin-Huxley studies have been instrumental for the prediction of biological mechanisms, which would otherwise not have been detectable by the use of the contemporary techniques. Their original studies have triggered further research, as well as the development of simplified models of reaction diffusion. The studies by Hodgkin and Huxley awarded them a Nobel Prize in physiology in 1963. The study had both dependent and ...Download file to see next pagesRead More
Cite this document
  • APA
  • MLA
(“Monitoring Synaptic Function at the Neuromuscular Junction of a Essay”, n.d.)
Monitoring Synaptic Function at the Neuromuscular Junction of a Essay. Retrieved from https://studentshare.org/biology/1451857-commentary-or-journal-club-paper-about-hodgkin-and
(Monitoring Synaptic Function at the Neuromuscular Junction of a Essay)
Monitoring Synaptic Function at the Neuromuscular Junction of a Essay. https://studentshare.org/biology/1451857-commentary-or-journal-club-paper-about-hodgkin-and.
“Monitoring Synaptic Function at the Neuromuscular Junction of a Essay”, n.d. https://studentshare.org/biology/1451857-commentary-or-journal-club-paper-about-hodgkin-and.
  • Cited: 0 times
Comments (0)
Click to create a comment or rate a document
Rapid Sequence Induction Neuromuscular Blocking Agents
A. Discuss the aims, indications for, and complications of Rapid Sequence Induction (RSI). Select appropriate drugs and dosage and include required treatment post B. Describe the action of depolarizing and non – depolarizing agents. Introduction Airway management is a core lifesaving skill for any health care worker, especially for those working in emergency situations.
5 Pages(1250 words)Essay
Case Study on Synaptic Corporation
In dealing with delay and dependency risks, I urge the IM management to apply the tool of avoidance. On the other hand, risk mitigation will be the best tool to be applied for the estimate risk.
8 Pages(2000 words)Essay
5. The likelihood of a particular syndrome correlates with viral load and CD4 lymphocyte counts. Acute inflammatory demyelinating polyradiculoneuropathy (Guillain-Barré syndrome) and isolated acute cranial
10 Pages(2500 words)Essay
Network Monitoring
Networking involves several devices which enable the relaying of required messages which include: routers/routing protocols. Essentially, computer networks are not a single entity which just describes a single type of networks but they involve several types of networks these being: extranet, intranet and internet.
7 Pages(1750 words)Essay
Synaptic pruning
114). The number of synapses decrease as a baby grows. This is because of a phenomenon termed as “synaptic pruning”. As a baby starts
2 Pages(500 words)Essay
As a point of departure, the roundabout junction provides a simple pedestrian visual since the traffic comes from one direction unlike the traffic and T-junctions, which involves
1 Pages(250 words)Essay
If the employer does not communicate such policies to the employee, then they have no right to monitor the internet usage of the employees. However, in most states, employers do not have an
1 Pages(250 words)Essay
The role of the neuromuscular system on work and exercise
Ehen one wants to move a particular part of the body, the message is sent to the spinal cord which is a part of the central nervous system and the response is sent via the motor neuron to the effectors organ to cause movement. Each muscle is served by around 50 t0 200
6 Pages(1500 words)Essay
Budjeting monitoring
In this case, Shultz and Open Society Institute (2005) note that overspending may be caused by misallocation of funds. Funds may have been overlooked by failing to budget for savings or reserves, failing to
1 Pages(250 words)Essay
The axon of the sensory neuron also extends from both directions: to receptors known as peripheral axon and to spinal cord known as the central axon (Swanson, 2012 p.124). Figure 2 shows the
4 Pages(1000 words)Coursework
sponsored ads
We use cookies to create the best experience for you. Keep on browsing if you are OK with that, or find out how to manage cookies.
Let us find you another Essay on topic Monitoring Synaptic Function at the Neuromuscular Junction of a MouseExpressing SynaptopHluorin for FREE!
logo footer
Contact us:
Contact Us Now
FREE Mobile Apps:
  • StudentShare App Store
  • StudentShare Google play
  • About StudentShare
  • Testimonials
  • FAQ
  • Blog
  • Free Essays
  • New Essays
  • Essays
  • Miscellaneous
  • The Newest Essay Topics
  • Index samples by all dates
Join us:
Contact Us