The principal aim of this paper is to introduce a research project that will investigate the molecular identity and expression patterns of the potassium-coupled chloride cotransporter KCC2 in cultured murine (caspase-activated deoxyribonuclease) CAD cell lines…
Download full paperFile format: .doc, available for editing
Download file to see previous pages
The paper, instead, expounds upon the seven cation-coupled chloride cotransporters that have been described to date with specific emphasis placed on KCC2. The cotransporter KCC2 is evident in neuronal processes with specific preference for inhibitory ones where ergic action is relevant. KCC2 action precedes the ergic one as the KCC2 action brings down the intracellular chloride concentration to optimum levels to make it possible for ergic action. The phylogenetic, molecular and structural-functional details of the cotransporters have also been provided with special emphasis on KCC2. The CAD cell line, new in studies like this, has been introduced. The cell line exhibits catecholaminergic voltage dependent currents across membranes. Specific experimentation details have not been provided. A 5708 nucleotide region of the SLC12A5 gene that encodes the KCC2 cotransporter protein was probed with two 20 bp bits, left and right primer details in Appendix, with RT-PCR analysis. The resulting 206-nucleotide insert was perfectly amplified with one complement in the RT-PCR at temperatures varying from C - C. mRNA isolated from undifferentiated and differentiated murine CAD cells were used for the analysis. Electrophoresis of the RT-PCR products through 1.5% agarose gel revealed that most of the inserts had been perfectly complemented once during the RT-PCR producing evidence that the SLC12A5 gene is present in both murine differentiated and undifferentiated CAD cells and that these cells can, thus, later be used as culture media for further study of the KCC2 cotransporter expression and molecular identity. This is of great importance to such study as a suitable cell line has been hard to find so far and the easy manner in which the gene revealed itself in this study assures that this cell line can be a very convenient medium of further such studies
1. Introduction 8-9
1.1 CAD Cells 9-10
1.2 The Cotransporters (KCC1, 2, 3 & 4) 10-11
1.3 The Electroneutral Cotransporters 11-12
1.4 Cotransporters KCC1 11-15
1.4.1 Genetic Details
1.4.3 Function and Location
1.5 Cotransporter KCC3 15-16
1.5.1 Genetic Details
1.6 Cotransporter KCC4 16-17
1.6.1 Molecular Genetics with Structural Identity
1.6.2 Function and Location
1.7 Cotransporters: General Features 17
1.8 Cotransporter KCC2 17-24
1.8.2 Structural and Locational Implications
1.8.3 Structural and Locational Implications
1.8.4 Specific Neuronal Location
1.8.5 Regulatory Action
1.8.7 Post- and PreNatal Concentration
1.8.8 Immunohistochemical Analysis
2.1 RNA Isolation Technique 24
2.2 RNA Isolation Protocol 24-27
2.3 Estimation of RNA Yield 27-29
2.3.1 Vol. Isolated RNA in Differentiated Cells
2.3.2 Vol. Isolated RNA in Undifferentiated Cells
2.4 Primer Design 29
2.5 The PCR Analysis 29-30
2.6 Gel Electrophoresis Technique 30-31
3. Results 31-41
4. Discussion 42-43
5. Conclusion 43-44
List of Abbreviations:
- Chloride anion
- GABA A type
- GABA S type
- Potassium cation
...Download file to see next pagesRead More
Cite this document
(“Molecular Identity Essay Example | Topics and Well Written Essays - 2500 words”, n.d.)
Molecular Identity Essay Example | Topics and Well Written Essays - 2500 words. Retrieved from https://studentshare.org/science/1528602-molecular-identity-essay
(Molecular Identity Essay Example | Topics and Well Written Essays - 2500 Words)
Molecular Identity Essay Example | Topics and Well Written Essays - 2500 Words. https://studentshare.org/science/1528602-molecular-identity-essay.
“Molecular Identity Essay Example | Topics and Well Written Essays - 2500 Words”, n.d. https://studentshare.org/science/1528602-molecular-identity-essay.
Other factors that determine gender identity include environmental situations, genetic factors, and psychological factors and sexual hormones. Gender identification is mostly consistent with sex in respect of chromosomes. Anyone destined to become a girl or a boy has their fate sealed the moment an ovum is fertilized by a sperm.
Initially, they had failed to attain the scores required of them to proceed to the next stage of learning that is why they had to join the vocational track for their studies (Rodriguez 45). Indeed, the learning environment was different as Ken found it. It changed their attitude and perception towards persons of different cultures.
Since it is used in a variety of different ways, it is not entirely clear what it means. Therefore, a number of psychological theories of identity have been used to define identity and explain the processes that produce it. This paper will therefore look at one approach to identity and how it can help to explain the identities of people with disabilities.
Identity theft has greatly increased with people using their personal information over the internet. When people buy something online with a credit card, or use any personal information online, no matte how secure the site says it is, there is a chance of identity theft.
These are all sodium/potassium-coupled chloride cotransporters described succinctly below. They can be functionally classified broadly into four groups according to the cation coupling to the chloride ion, the stoichiometry of the transport process and the relative sensitivities to inhibitors.
Social psychology weighs identity more on the behavioral side perhaps because it has a lot to do with the concept of behavior functions and identity co-operation. which primarily means, that a person has to co operate with a society as a whole concerning the actual meaning of his/her own identity along with the identification of societal needs according to his/her personal traits which can eventually help the society in identifying a person's singular identity.
The author states that synapsins are considered essential for intense vesicle recruitment during sustained transmission across relevant synapses. They are believed to have multiple functions within presynaptic terminals. Besides recruiting synaptic vesicles (SVs) they also regulate their fusion.
However, the development of civilization influences even this category of gender identity. In the XXI century when society does not expect much from men and women this identity passes through obvious transformation. The film “Romper Stomper” is a perfect example
6 Pages(1500 words)Essay
GOT A TRICKY QUESTION? RECEIVE AN ANSWER FROM STUDENTS LIKE YOU!
Save Your Time for More Important Things
Let us write or edit the essay on your topic
"Molecular Identity Essay"
with a personal 20% discount.