The Microbial Organisms - Lab Report Example

Download file to see previous pages The basic kinds of agar display that were used included, Mannitol Salt Agar for Staphylococcus aureus, MacConkey Agar for Enterobacteriaceae spa, Slants & Bartley agar for Enterococcus sap, and MRS Agar for Lactobacillus sp. Subsequently, we observed the commensal microbial growth in 4 weaken diabetic rats along with that in normal rats for obtaining comparative figures through several confirmation tests including Catalase test, Coagulase Test, RAPIDash Test, Oxidase test and, API 20E Test. Significant findings showed that the diabetic rats weighed more than the normal rats. The stool of the Ileum and colon of normal and diabetic rats were sent to investigate the microbial aberrancies, after the induction of streptozotocin. Consequently, there was an incidental increase in GI tract microbes. The results showed that diabetes in rats was caused 3 days after streptozotocin.  Type 1 Diabetes (T1D) is a condition where the level of blood glucose rises due to the absence insulin. Here either insulin is not formed or if formed then the amount is very little. This condition usually occurs under the age of 30 and the patient requires insulin injections for life. Type 1 Diabetes is known insulin-dependent juvenile-onset diabetes. Type 1 diabetes is associated with several autoimmune conditions. The strongest association is with Celiac disease, Hashimoto's disease, or hypothyroidism; Graves' disease, or hyperthyroidism; Addison's disease, or adrenal failure, and Pernicious anemia The link between the GI tract immune system and T1D has been suggested by studies that have demonstrated that dietary factors modify the disease in animal models of autoimmune diabetes (Vaarala, 2004). These microbial organisms play an essential role in the anaerobic down of carbohydrates and protein molecules, thereby affecting the energy storage mechanism in the hosts. 

Indigestible dietary fibers are broken into Short Chain Fatty Acids (SCFA) by these commensal microbes and polysaccharides are efficiently broken down into no saccharide units prior to be absorbed from the lumen of the gastrointestinal tract for subsequent hepatic lipogenesis (Backed et al., 2004).  These metabolites that are end products of bacterial degradation are important for colonel activity causing motility and secretion during digestive processes (Scheppach, 1994).

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