We use cookies to create the best experience for you. Keep on browsing if you are OK with that, or find out how to manage cookies.
Nobody downloaded yet

Cellular pathology critical review assignment - Essay Example

Comments (0)
While it has been shown that bovine E5 protein impairs the synthesis, stability and transport of the major histocompatibility (MHC) type I complex, this study shows that the E5 protein expressed…
Download full paper
Cellular pathology critical review assignment
Read TextPreview

Extract of sample
Cellular pathology critical review assignment

Download file to see previous pages... ules A and B, which present viral peptides to the cytotoxic T-lymphocytes, thereby avoiding their clearance from the body which leads to establishment of infection and later malignancy. For the in vitro study immortalized human keratinocyte cell line HaCaT, primary bovine PalF cells and NIH 3T3 cell lines which expressed the HPV-16 E5 protein was used and some NIH 3T3 expressed HPV-6 E5. After subsequent culture, suitable immunological assays were carried out to detect the presence and localization of the MHC class I molecules in these cells. While in the control cell lines MHC molecules were localized both in the cell surface and within the GA, in the HPV-16 E5 expressing cells the molecules were found exclusively in the GA. The study also showed that HPV-16 E5 did not affect the synthesis of MHC molecules unlike BPV E5 and that the expression of the E5 protein did no affect the transporter protein responsible for the transport of MHC molecules. To determine the effect of interferon, all the cell lines were treated with interferon β, which resulted in all the cell lines having similar levels of MHC molecules and also the molecules were found on the cell surface in the E5 expressing cell lines thus proving that the inhibition on transport of MHC molecules is reversed upon treatment with interferon. The study also noted that E5 did not down regulate the expression of HLA-C and E, involved in inhibiting NK cell-mediated lysis.
The two most striking limitations observed is: the study was carried out in vitro and in vivo reproducibility needs to be ascertained and secondly, the detection of the E5 protein was carried out by RT-PCR rather simpler techniques. The two major strengths include: this is the first paper to show the immune evasion mechanism by the HPV E5 protein and the cell lines used are a good model for understanding the mechanism and finally, the study explores the gene level expressions involved in the down regulation process of the MHC molecules.
In ...Download file to see next pagesRead More
Comments (0)
Click to create a comment or rate a document
Cellular and Molecular ( Pathology Case Study Assignment)
ve of the diseased organ. Recently guidelines suggest an adequate biopsy specimen to be 20- 25 mm long and consisting of 11 complete portal tracts (Cholongitas et al, 2006); this might be less when it comes to focal lesions, such as in this particular patient.
6 Pages(1500 words)Essay
CELLULAR PATHOLOGY- Staining protocols
Glycogen is preserved in the slides using the alcoholic preservation methods. The use of formalin solution will help to reduce the shrinkage and polarization of the tissue. Periodic Acid Schiff (PAS) stain is the most commonly used stain to identify the structures containing large amounts of glycogen, glycoprotein, mucin, proteoglycan and other basement membranes.
3 Pages(750 words)Essay
CELLULAR PATHOLOGY- Staining protocols
Liver slides for glycogen and ileum slides for mucin staining (using Alcian Blue) were prepared as well with use of diastase digestion. Introduction The Periodic Acid Schiff (PAS) stain is a stain routinely employed in several cellular pathology laboratories.
4 Pages(1000 words)Essay
Cellular and Molecular Pathology
Since this case involves a human being and thus human materials, which is a scarce situation. Surgical materials or rather tissues are the best source of human tissue due to the ability to remove the normal tissue together with the affected/diseased tissues.
6 Pages(1500 words)Essay
2 Pages(500 words)Essay
Cellular pathology(critical review of topical paper)
le aspiration samples from histologically confirmed metaplastic carcinomas of breast, obtained from 6 different institutions and comparing them with the already present histopathological results. Considering the fact that the malignancy is a rare occurrence, the number of cases
2 Pages(500 words)Essay
Cellular pathology
In the first section of the tissue examination, Haematoxylin and Eosin stain is used to give a good visibility of the cells and nucleus of the tissue and their activity state. Haematoxylin is a dye that is
3 Pages(750 words)Essay
Cellular pathology 2
The cytoplasmic vacuoles of these cells appear relatively uniform, and intra-nuclear vacuoles are present. The cytoplasmic amount is small owing to the accumulation of messenger RNA and ribosomal RNA.
3 Pages(750 words)Essay
Cellular pathology 1
Neutrophil infiltration can also be appreciated in submucosa and muscularis externa. Diagram also shows aggregates of lymphoid follicles which can be appreciated by the purple colour they have taken up after staining. H&E staining have stained an organism in the second
3 Pages(750 words)Essay
Cellular pathology
Upon graduating from college of sciences, I had the opportunity to work at King Abdul Aziz hospital in 2008. I aspire to continue with my studies in medical diagnostics at the University. Based on my laboratory science background and
2 Pages(500 words)Personal Statement
Let us find you another Essay on topic Cellular pathology critical review assignment for FREE!
Contact us:
Contact Us Now
FREE Mobile Apps:
  • About StudentShare
  • Testimonials
  • FAQ
  • Blog
  • Free Essays
  • New Essays
  • Essays
  • The Newest Essay Topics
  • Index samples by all dates
Join us:
Contact Us