Nobody downloaded yet

Cellular pathology critical review assignment - Essay Example

Comments (0) Cite this document
Summary
While it has been shown that bovine E5 protein impairs the synthesis, stability and transport of the major histocompatibility (MHC) type I complex, this study shows that the E5 protein expressed…
Download full paperFile format: .doc, available for editing
GRAB THE BEST PAPER98.9% of users find it useful
Cellular pathology critical review assignment
Read TextPreview

Extract of sample
"Cellular pathology critical review assignment"

Download file to see previous pages ules A and B, which present viral peptides to the cytotoxic T-lymphocytes, thereby avoiding their clearance from the body which leads to establishment of infection and later malignancy. For the in vitro study immortalized human keratinocyte cell line HaCaT, primary bovine PalF cells and NIH 3T3 cell lines which expressed the HPV-16 E5 protein was used and some NIH 3T3 expressed HPV-6 E5. After subsequent culture, suitable immunological assays were carried out to detect the presence and localization of the MHC class I molecules in these cells. While in the control cell lines MHC molecules were localized both in the cell surface and within the GA, in the HPV-16 E5 expressing cells the molecules were found exclusively in the GA. The study also showed that HPV-16 E5 did not affect the synthesis of MHC molecules unlike BPV E5 and that the expression of the E5 protein did no affect the transporter protein responsible for the transport of MHC molecules. To determine the effect of interferon, all the cell lines were treated with interferon β, which resulted in all the cell lines having similar levels of MHC molecules and also the molecules were found on the cell surface in the E5 expressing cell lines thus proving that the inhibition on transport of MHC molecules is reversed upon treatment with interferon. The study also noted that E5 did not down regulate the expression of HLA-C and E, involved in inhibiting NK cell-mediated lysis.
The two most striking limitations observed is: the study was carried out in vitro and in vivo reproducibility needs to be ascertained and secondly, the detection of the E5 protein was carried out by RT-PCR rather simpler techniques. The two major strengths include: this is the first paper to show the immune evasion mechanism by the HPV E5 protein and the cell lines used are a good model for understanding the mechanism and finally, the study explores the gene level expressions involved in the down regulation process of the MHC molecules.
In ...Download file to see next pagesRead More
Cite this document
  • APA
  • MLA
  • CHICAGO
(“Cellular pathology critical review assignment Essay”, n.d.)
Cellular pathology critical review assignment Essay. Retrieved from https://studentshare.org/miscellaneous/1563369-cellular-pathology-critical-review-assignment
(Cellular Pathology Critical Review Assignment Essay)
Cellular Pathology Critical Review Assignment Essay. https://studentshare.org/miscellaneous/1563369-cellular-pathology-critical-review-assignment.
“Cellular Pathology Critical Review Assignment Essay”, n.d. https://studentshare.org/miscellaneous/1563369-cellular-pathology-critical-review-assignment.
  • Cited: 0 times
Comments (0)
Click to create a comment or rate a document
CHECK THESE SAMPLES - THEY ALSO FIT YOUR TOPIC
Cellular and Molecular ( Pathology Case Study Assignment)
...?Cellular and Molecular Pathology (Case Study Assignment) Tissue histopathological studies have become the gold standard for the diagnosis of a variety of liver diseases. According to the generally accepted criteria, following are the indications for liver biopsy: chronic hepatitis, Wilson`s disease, alcoholic hepatitis, non-alcoholic steatohepatitis, primary biliary cirrhosis, cholestatic liver disease, fever of unknown origin, liver mass, status of liver post-transplantation and of donor pre-transplantation (Bravo et al, 2001) Though it is generally accepted that liver biopsy specimen is no more than 1/150,000 part of the whole liver mass, but this is considered to be adequately...
6 Pages(1500 words)Essay
CELLULAR PATHOLOGY- Staining protocols
...? CELLULAR PATHOLOGY- Staining protocols Glycogen Glycogen is present in all the animals it the liver, with liver storing the glucose in the form of glycogen. Thus, during starvation or whenever glucose is required by the body, glycogen is converted back into glucose. Glycogen is preserved in the slides using the alcoholic preservation methods. The use of formalin solution will help to reduce the shrinkage and polarization of the tissue. Periodic Acid Schiff (PAS) stain is the most commonly used stain to identify the structures containing large amounts of glycogen, glycoprotein, mucin, proteoglycan and other basement membranes. (McGavin and Zachary 2006). Fig1: Periodic acid Schiff stain (PAS) of kidney....
3 Pages(750 words)Essay
CELLULAR PATHOLOGY- Staining protocols
...?Cellular Pathology experiment The aim of the practical was to look at sections of small intestine (ileum), kidney and liver. These are sections that were already processed to paraffin wax, sectioned, fixed as well as mounted on slides. Liver slides for glycogen and ileum slides for mucin staining (using Alcian Blue) were prepared as well with use of diastase digestion. Introduction The Periodic Acid Schiff (PAS) stain is a stain routinely employed in several cellular pathology laboratories. Several tissue components can be demonstrated by this stain. It is an important diagnostic tool in most clinical settings. Substances that consist of glycol groups or alkylamino and...
4 Pages(1000 words)Essay
Cellular and Molecular Pathology
...? Cellular and molecular pathology The most suitable method of removing adequate tissue for histopathological examinationand the most reliable way of making sure you get the correct tissue needed Materials to be used in histological preparations are removed from anaesthetized animals. Since this case involves a human being and thus human materials, which is a scarce situation. Surgical materials or rather tissues are the best source of human tissue due to the ability to remove the normal tissue together with the affected/diseased tissues. In obtaining the right tissue for the examination, there are two distinct methods that can be employed. That is the physical examination technique and the liver biopsy technique. During physical... -heme...
6 Pages(1500 words)Essay
CELLULAR PATHOLOGY CERVICAL CYTOLOGY
...CELLULAR PATHOLOGY CERVICAL CYTOLOGY OBSERVING THE COLOUR TRANSPARENCIES AND READING PRACTICAL SCHEDULE where is squamous epithelium located in the vagina and cervix [2 MARKS] Vagina - vaginal canal Cervix - exterior surface [2] where is the columnar epithelium located in the cervix [1 MARK] Endocervical canal [3] list some cell types found in a normal cervical smear [6 MARKS] Squamous cells - basal, parabasal, intermediate and superficial cells, columnar cells, blood cells [4] what colours do these cell types stain with the papanicolaou stain [6 MARKS] Parabasal - stain green [denser] Intermediate - stain green [ pale] Superficial - stain pink Basal cells - dense green Blood cells- orange [5] name some contaminants found... in...
2 Pages(500 words)Essay
Cellular pathology(critical review of topical paper)
...Critical Review The purpose of the study by Lui et al (2006) was to assess the diagnostic cytological features of metaplastic carcinoma of the breast and throw light on alternative and less invasive diagnostic options. The study was conducted by reviewing 17 cytological preparations of fine needle aspiration samples from histologically confirmed metaplastic carcinomas of breast, obtained from 6 different institutions and comparing them with the already present histopathological results. Considering the fact that the malignancy is a rare occurrence, the number of cases studied can be ascertained as ‘sufficient’. Based on the cellularity and atypia, 3 categories of...
2 Pages(500 words)Essay
Malignancy (Cellular Pathology)
...al Affiliation) MALIGNANCY (CELLULAR MORPHOLOGY) Normal Tissue Present Firstly, normal adipose tissue in the image are the ones on the right; the ones that appear lighter. Usually, staining (as performed in the image) helps to distinguish normal from cancerous tissue. From the image one can see that compared to the adipose tissue that appears darker, the normal adipose tissue is smaller (Koch 2010, pg. 18). The normal squamous cells are also consistent in their size and shape, unlike the cancerous squamous cells which appear to have very different shapes and sizes. Using the image only, those are the clear observations that can be made when describing the normal adipose tissue. In addition, unlike the cancerous squamous...
3 Pages(750 words)Case Study
Cellular pathology
...Cellular pathology Cellular pathology Gastritis is a condition with the lining of the stomach called mucosa that becomes inflamed. There are special cells contained on the stomach lining that produce enzymes and acid helping in the digestion and breakdown of food. The mucus from the acid protects the lining of the stomach and gastritis leads to less production of enzymes, acid and mucus. In the first section of the tissue examination, Haematoxylin and Eosin stain is used to give a good visibility of the cells and nucleus of the tissue and their activity state. Haematoxylin is a dye that is dark purple that will stain the nuclear material of the tissue making it a deep...
3 Pages(750 words)Essay
Cellular pathology 1
...CELLULAR PATHOLOGY CELLULAR PATHOLOGY PATHOLOGICAL FEATURES The specimens have been stained with hematoxylin and eosin (H&E) which is the commonest stain used in light microscopy because it stains various structures quite simply(Ovalle et al, 2013, p.g.479). The diagrams show signs of inflammation. Mucosa is damaged and crypts can be seen. Neutrophil infiltration can also be appreciated in submucosa and muscularis externa. Diagram also shows aggregates of lymphoid follicles which can be appreciated by the purple colour they have taken up after staining. H&E staining have stained an organism in the second picture, which is enterrobius vermicularis. The...
3 Pages(750 words)Essay
Cellular pathology
...Cellular Pathology As a young aspiring scientist, I wish to obtain my Master’s Degree in Cellular Pathology from West of England University. Currently, I am a finalist in Biomedical Science level in the UK. Prior to my studies in UK, I attained an intermediate University Degree in Laboratory Sciences from college of health sciences. Upon graduating from college of sciences, I had the opportunity to work at King Abdul Aziz hospital in 2008. I aspire to continue with my studies in medical diagnostics at the University. Based on my laboratory science background and experience I obtained from King Abdul Aziz Hospital, I have confidence that I possess the necessary skills...
2 Pages(500 words)Personal Statement
sponsored ads
We use cookies to create the best experience for you. Keep on browsing if you are OK with that, or find out how to manage cookies.
Let us find you another Essay on topic Cellular pathology critical review assignment for FREE!
Contact Us